Geographic Variance in the Frequency of the t(14;18) Translocation in Follicular Lymphoma: An Israeli Series Compared to the World Literature

Segel, Michael J.; Paltiel, Ora; Zimran, Ari; Gottschalk-Sabag, S; Schibi, Ginette; Krichevski, Svetlana; Ludkovski, Olga; Yehuda, Dina Ben

doi:10.1006/bcmd.1998.0171

Cited by 17 publications

(13 citation statements)

References 41 publications

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“…Overall, the positivity of the three sets of primers is suggestive of an unmutated status of the V H gene of the lymphoma cells (i.e., pre-GC B-cell origin, such as mantle cell lymphoma), whereas the positivity of FR1c/J H and/or FR2a/J H together with the negativity of FR3a/J H is mainly found in lymphomas of GC (such as FL) and post-GC B-cell origin. In FL, the detection of bcl-2/J H rearrangement is a diagnostic adjunct, but only 50% were found to be positive with sets of primers detecting MBR and mcr, which is comparable to the results reported in other European series (37,38).…”

Section: Discussionsupporting

confidence: 86%

PCR Analysis of Immunoglobulin Heavy Chain (IgH) and TcR-γ Chain Gene Rearrangements in the Diagnosis of Lymphoproliferative Disorders: Results of a Study of 525 Cases

et al. 2000

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This report summarizes a cumulative 4-year experience in polymerase chain reaction (PCR) analysis of immunoglobin heavy chain (IgH) and TcR-␥ chain gene rearrangements in 525 cases of lymphoproliferative disorders. Because the sensitivity of the PCR methodology was found to be tissue dependent, in the study of the presence of clonal cell population in tissues containing a small number of polyclonal lymphocytes, such as skin and gastrointestinal biopsy specimens, we used the multiple-PCR run approach. In this latter methodology, we repeat the PCR reaction from the same sample at least three times to confirm the reproducibility of the results. In the study of 273 cases of B-or T-cell lymphomas with characteristic immunomorphological and clinical features, a clonal IgH or TcR-␥ chain gene rearrangement was detected in approximately 80% of cases. A clonal rearrangement involving both IgH and TcR-␥ chain genes was found in 10% of cases of both B-cell and T-cell lymphomas. The study of 167 cases of nonneoplastic lymphoid tissue samples showed the presence of clonally rearranged cell populations for IgH or TcR-␥ genes in 3 and 9% of cases, respectively. We also applied PCR for the study of 85 cases of lymphoproliferations with no definite diagnosis (i.e., benign versus malignant) after immunomorphological analysis. In 65 cases (76%), the correlation of immunomorphological features with the presence (48 cases) or the absence (17 cases) of clonal lymphoid cell populations led to a definite diagnosis. In almost all these cases, the final diagnosis was found to be in agreement with the clinical course. In the 20 remaining cases (24%), no definite diagnosis could be made. We also assessed the value of PCR in detecting bcl-2/J H gene rearrangement as an additional clonal marker in the diagnosis of follicular lymphoma. Bcl-2/J H rearrangement and/or IgH gene rearrangement was found in approximately 85% (71/85) of follicular lymphoma cases studied.

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Section: Discussionsupporting

confidence: 86%

PCR Analysis of Immunoglobulin Heavy Chain (IgH) and TcR-γ Chain Gene Rearrangements in the Diagnosis of Lymphoproliferative Disorders: Results of a Study of 525 Cases

et al. 2000

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“…Hence, it contradicts previous claims of a low frequency of t(14;18) translocation in Asian FL, which was believed to be related to the rarity of this lymphoma in Asian countries, attributed to geographical, racial and environmental factors 3,38 . Our results support the notion that the t(14;18) translocation is a common molecular anomaly in all FLs irrespective of geographical location, 1,45 and the difference observed between the East and West in the past may be due in part to technical factors 46 …”

Section: Discussionsupporting

confidence: 59%

The pattern and frequency of t(14;18) translocation and immunophenotype in Asian follicular lymphoma

Peh¹,

Shaminie²,

Tai³

et al. 2004

Histopathology

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The frequency of t(14;18) translocation in this series of follicular lymphomas was higher when compared with previous Asian reports, but in accordance with European and North American findings. CD10 expression is strongly associated with a t(14;18) translocation event, but the overall CD10 expression was relatively low, possibly due to the high proportion of high-grade tumours in the series. t(14;18) translocation was not associated with Bcl-2 or Bcl-6 expression.

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“…Geographic differences in the incidence of IGH translocations have been reported (Segel et al, 1998), with a lower frequency in Japan (Maseki et al, 1987;Konishi et al, 1990;Takechi et al, 1991;Hashimoto et al, 1995). Using interphase FISH with an IGH probe, Ueda et al (1996), Tsujimoto et al (1984b) and Taniwaki et al (1995) found IGH rearrangements in 41% of the Japanese B-cell NHL patients studied.…”

Section: Discussionmentioning

confidence: 99%

Molecular cytogenetics of IGH rearrangements in non-Hodgkin B-cell lymphoma

Bernicot

Douet‐Guilbert²,

Bris³

et al. 2007

Cytogenet Genome Res

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Rearrangements involving the IGH gene have been identified in about 50% of non-Hodgkin B-cell lymphomas (NHLs) and correlated to clinically relevant subgroups. However, the detection rate largely varied with the technique used. We analyzed the incidence of IGH rearrangements using several fluorescence in situ hybridization (FISH) techniques on metaphases obtained from 96 patients with nodal NHL. An IGH rearrangement was identified in 71 cases (74%). A t(14;18)(q32;q21) was found in 37 of the 42 follicular lymphomas (88.1%) studied and a t(11;14)(q13;q32) in 12 of the 14 mantle cell lymphomas (85.7%). IGH rearrangements were identified in 21 of the 40 diffuse large B-cell lymphomas (52.5%), including seven t(14;18)(q32;q21) and four t(3;14)(q27;q32). Conventional cytogenetics was uninformative in several cases. However, the complemented analysis using 24-color FISH, chromosomal whole paints, telomeric probes and locus specific identifiers enabled us to characterize complex and/or masked IGH translocations in follicular lymphomas and mantle cell lymphomas and to identify all the chromosomal partners involved in IGH rearrangements in diffuse large B-cell lymphomas. This study shows the interest of using metaphase FISH in addition to conventional cytogenetics. Following banding techniques, FISH with the IGH dual color probe can be the first approach in NHL, after which chromosome painting and 24-color FISH can be used to identify the chromosomal partners involved in IGH rearrangements. The identification of these genes is of utmost importance for a better understanding of the molecular mechanisms involved in the genesis of lymphoma.

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Geographic Variance in the Frequency of the t(14;18) Translocation in Follicular Lymphoma: An Israeli Series Compared to the World Literature

Cited by 17 publications

References 41 publications

PCR Analysis of Immunoglobulin Heavy Chain (IgH) and TcR-γ Chain Gene Rearrangements in the Diagnosis of Lymphoproliferative Disorders: Results of a Study of 525 Cases

PCR Analysis of Immunoglobulin Heavy Chain (IgH) and TcR-γ Chain Gene Rearrangements in the Diagnosis of Lymphoproliferative Disorders: Results of a Study of 525 Cases

The pattern and frequency of t(14;18) translocation and immunophenotype in Asian follicular lymphoma

Molecular cytogenetics of IGH rearrangements in non-Hodgkin B-cell lymphoma

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