Geographic Cline and Genetic Introgression Effects on Seed Morphology Variation and Germination Fitness in Two Closely Related Pine Species in Southeast Asia

Abstract

There is still limited information on how genetic introgression impacts morphological variation and population fitness in long-lived conifer species. Two closely related pine species, Pinus kesiya Royle ex Gordon and Pinus yunnanensis Franch. are widely distributed over Southeast Asia and Yunnan province of China, with a large spatial scale of asymmetric genetic introgression and hybridization, and form a hybrid lineage, P. kesiya var. langbianensis, where their ranges overlap in southeast Yunnan. We compared seed trait variation and germination performance between hybrids and parental species and characterized environmental gradients to investigate the genetic and ecological evolutionary consequences of genetic introgression. We found that seed width (SW) differed significantly among the three pines, and all the seed traits were significantly negatively correlated with latitude and associated with the mean temperatures of the driest and wettest quarters. A higher germination fitness of hybrids was detected at a low temperature, indicating that they had better adaptability to temperature stress than their parental species during the germination process. Our results suggest that environmental factors shape seed phenotypic variation in the pine species and that genetic introgression significantly affects seed germination fitness. Therefore, assisting gene flow in natural forest populations might facilitate their adaptation to climate change.