Geodermatophilus arenarius sp. nov., a xerophilic actinomycete isolated from Saharan desert sand in Chad

Abstract

A novel Gram-positive, aerobic, actinobacterial strain, CF5/4(T), was isolated in 2007 during an environmental screening of arid desert soil in Ouré Cassoni, Chad. The isolate grew best in a temperature range of 28-40 °C and at pH 6.0-8.5, with 0-1 % (w/v) NaCl, forming brown-coloured and nearly circular colonies on GYM agar. Chemotaxonomic and molecular characteristics of the isolate matched those described for members of the genus Geodermatophilus. The DNA G + C content of the novel strain was 75.9 mol %. The peptidoglycan contained meso-diaminopimelic acid as diagnostic diaminoacid. The main phospholipids were phosphatidylethanolamine, phosphatidylcholine, phosphatidylinositol, diphosphatidylglycerol and a small amount of phosphatidylglycerol; MK-9(H(4)) was identified as the dominant menaquinone and galactose as diagnostic sugar. The major cellular fatty acids were branched-chain saturated acids: iso-C(15:0) and iso-C(16:0). The 16S rRNA gene showed 96.2-98.3 % sequence identity with the three members of the genus Geodermatophilus: G. obscurus (96.2 %), G. ruber (96.5 %), and G. nigrescens (98.3 %). Based on the chemotaxonomic results, 16S rRNA gene sequence analysis and DNA-DNA hybridization with the type strain of G. nigrescens, the isolate is proposed to represent a novel species, Geodermatophilus arenarius (type strain CF5/4(T) = DSM 45418(T) = MTCC 11413(T) = CCUG 62763(T)).

Blastococcus capsensis sp. nov., isolated from an archaeological Roman pool and emended description of the genus Blastococcus, B. aggregatus, B. saxobsidens, B. jejuensis and B. endophyticus

“…Growth of BMG 804 T in the presence of 0-10 % NaCl (w/v) was determined on yeast extract-malt extract medium (ISP 2). Additionally, production of indole, nitrate reduction and hydrolysis of tyrosine (Gordon & Smith, 1955), casein, starch, xanthine, hypoxanthine (Montero-Calasanz et al, 2012), gelatin (Clarke, 1953) and aesculin (Swan, 1954) were tested.…”

Stone-dwelling actinobacteria Blastococcus saxobsidens, Modestobacter marinus and Geodermatophilus obscurus proteogenomes

“…Surprisingly, Geodermatophilaceae are present in a variety of biotopes including prominently rocks (Eppard et al, 1996) and desert sandy soils (Montero-Calasanz et al, 2012, 2013a, 2013c, 2013dLiu et al, 2014). Although considered endemic to soils, evolution of Geodermatophilaceae has continued in specialized land biotopes.…”

Geodermatophilus poikilotrophisp. nov.: A Multitolerant Actinomycete Isolated from Dolomitic Marble

“…Fourteen named species have been classified in the genus Geodermatophilus (ordered by the dates of effective publication of the names): G. obscurus [3], G. ruber [9], G. nigrescens [17], G. arenarius [18], G. siccatus [19, 20], G. saharensis [20, 21], G. tzadiensis [22, 23], G. telluris [24], G. soli and G. terrae [10], G. africanus [5, 23], G. normandii [25], G. taihuensis [26], and G. amargosae [27, 28]. Until now, only the genome of the type strain of the type species, G. obscurus G-20 T , has been sequenced [29].…”