Lake Albano (Alban Hills volcanic complex, Central Italy) is located in a densely populated area near Rome. The deep lake waters have significant dissolved CO 2 concentrations, probably related to sub-lacustrine fluid discharges fed by a pressurized CO 2 -rich reservoir. The analytical results of geochemical surveys carried out in 1989-2010 highlight the episodes of CO 2 removal from the lake. The total mass of dissolved CO 2 decreased from ∼5.8× 10 7 kg in 1989 to ∼0.5×10 7 kg in 2010, following an exponential decreasing trend. Calculated values of both dissolved inorganic carbon and CO 2 concentrations along the vertical profile of the lake indicate that this decrease is caused by CO 2 release from the epilimnion, at depth <9 m, combined with (1) water circulation at depth <95 m and (2) CO 2 diffusion from the deeper lake layers. According to this model, Lake Albano was affected by a large CO 2 input that coincided with the last important seismic swarm at Alban Hills in 1989, suggesting an intimate relationship between the addition of deep-originated CO 2 to the lake and seismic activity. In the case of a CO 2 degassing event of an order of magnitude larger than the one that occurred in 1989, the deepest part of Lake Albano would become CO 2 -saturated, resulting in conditions compatible with the occurrence of a gas outburst. These results reinforce the idea that a sudden CO 2 input into the lake may cause the release of a dense gas cloud, presently representing the major volcanic threat for this densely populated area.