Geochemical and microbiological characteristics of sediments near the Malenky mud volcano (Lake Baikal, Russia), with evidence of Archaea intermediate between the marine anaerobic methanotrophs ANME-2 and ANME-3

Zemskaya, Т. I.; Pogodaeva, T. V.; Shubenkova, O. V.; Сhernitsina, Svetlana M.; Дагурова, О. П.; Buryukhaev, S. P.; Намсараев, Б. Б.; Khlystov, Oleg; Егоров, А. А.; Krylov, Alexey; Kalmychkov, G. V.

doi:10.1007/s00367-010-0199-6

Cited by 37 publications

(17 citation statements)

References 56 publications

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“…It may also in part reflect the locally different geochemical conditions in areas affected by groundwater inflows entering the lake at different depths. A similar influence was proposed by Zemskaya and coworkers [68] to explain the high diversity of Archaea in sediments from a mud volcano in southern Lake Baikal.…”

Section: Environmental Euryarchaeotamentioning

confidence: 67%

Local Conditions Structure Unique Archaeal Communities in the Anoxic Sediments of Meromictic Lake Kivu

et al. 2012

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Section: Environmental Euryarchaeotamentioning

confidence: 67%

Local Conditions Structure Unique Archaeal Communities in the Anoxic Sediments of Meromictic Lake Kivu

et al. 2012

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“…Within 3 h of core retrieval, the porewater samples were extracted from the sediments by centrifugation and stored at 4 °C. Measurements of porewater anion and cation concentrations were carried out according to previously described techniques (Zemskaya et al ., ).…”

Section: Methodsmentioning

confidence: 97%

Microbial community structure in methane hydrate-bearing sediments of freshwater Lake Baikal

Kadnikov

Mardanov

Beletsky

et al. 2011

FEMS Microbiol Ecol

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Gas hydrates in marine sediments have been known for many years but recently hydrates were found in the sediments of Lake Baikal, the largest freshwater basin in the world. Marine gas hydrates are associated with complex microbial communities involved in methanogenesis, methane oxidation, sulfate reduction and other biotransformations. However, the contribution of microorganisms to the formation of gas hydrates remains poorly understood. We examined the microbial communities in the hydrate-bearing sediments and water column of Lake Baikal using pyrosequencing of 16S rRNA genes. Aerobic methanotrophic bacteria dominated the water sample collected at the lake floor in the hydrate-bearing site. The shallow sediments were dominated by Archaea. Methanogens of the orders Methanomicrobiales and Methanosarcinales were abundant, whereas representatives of archaeal lineages known to perform anaerobic oxidation of methane, as well as sulfate-reducing bacteria, were not found. Affiliation of archaea to methanogenic rather than methane-oxidizing lineages was supported by analysis of the sequences of the methyl coenzyme M reductase gene. The deeper sediments located at 85-90 cm depth close to the hydrate were dominated by Bacteria, mostly assigned to Chloroflexi, candidate division JS1 and Caldiserica. Overall, our results are consistent with the biological origin of methane hydrates in Lake Baikal.

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“…Pore water extractions were obtained from 9 samples (100 g each), which were collected throughout the core. Pore waters were obtained on board the ship by sequential centrifuging the sample for 20 min at 5000 g, then for 10 min at 12,000 g. The pore water anion and cation contents were determined the methods described previously and are only briefly repeated here [57,65,66]. Pore water anion (SO 4 2− , NO 3 − , HCO 3 − , and CH 3 COO − ) concentrations were measured onboard the ship by means of liquid chromatography immediately after preparation (5-10% relative error).…”

Section: Measurements Of Methane Concentrations and Analysis Of The Cmentioning

confidence: 99%

“…In deep sediments near GH, the rate of AOM ranged from 35 to 273.2 µL CH 4 /(dm 3 day) [53], increasing at depths of 20-60 cm, which may indicate the functioning of the anaerobic community of microorganisms [54][55][56]. The process of AOM in deep sediments of Lake Baikal was detected by radioisotope techniques, with a maximum that was usually close to the GH layers, as well as by the shape of methane profile with depth typical for AOM [56,57]. Despite high methane concentrations in deep sediments of some sites of Lake Baikal (up to 15000 µM) that are sufficient to carry out of AOM [58], members of methane-oxidizing ANME clusters were not detected [57,59,60].…”

Section: Introductionmentioning

confidence: 99%

“…The process of AOM in deep sediments of Lake Baikal was detected by radioisotope techniques, with a maximum that was usually close to the GH layers, as well as by the shape of methane profile with depth typical for AOM [56,57]. Despite high methane concentrations in deep sediments of some sites of Lake Baikal (up to 15000 µM) that are sufficient to carry out of AOM [58], members of methane-oxidizing ANME clusters were not detected [57,59,60]. In this regard, it was suggested that the process could be carried out in methane-enriched freshwater lake sediments by other microorganisms, including ANME-2d archaea and NC10 bacteria.…”

Section: Introductionmentioning

confidence: 99%

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Diversity of NC10 Bacteria and ANME-2d Archaea in Sediments of Fault Zones at Lake Baikal

et al. 2019

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Anaerobic oxidation of methane (AOM) reduces methane emissions from marine ecosystems, but we know little about AOM in freshwater reservoirs. Lake Baikal is the world’s only freshwater reservoir where gas hydrates (GH) are found. Despite that AOM has been demonstrated in deep sediments of Lake Baikal did not reveal methane-oxidizing archaea ANME1, 2, or 3, which are responsible for AOM in marine sediments. A search for representatives Candidatus Methylomirabilis oxyfera (M. oxyfera)-like bacteria (NC10) and Candidatus Methanoperedens nitroreducens (M. nitroreducens)-like archaea (ANME-2d) has been carried out in the different types of Lake Baikal methane enriched sediments. We used different molecular biology methods including high-throughput sequencing and PCR analysis, using 16S rRNA genes as well as different functional genes of AOM (mcrA and pmoA). We found diverse M. oxyfera-like bacteria and M. nitroreducens-like archaea in various geological structures in Lake Baikal (methane seep and mud volcano), which were different from the composition of the discharged fluid. We also considered possible electron acceptors for this process in the sediments of Lake Baikal.

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Geochemical and microbiological characteristics of sediments near the Malenky mud volcano (Lake Baikal, Russia), with evidence of Archaea intermediate between the marine anaerobic methanotrophs ANME-2 and ANME-3

Cited by 37 publications

References 56 publications

Local Conditions Structure Unique Archaeal Communities in the Anoxic Sediments of Meromictic Lake Kivu

Local Conditions Structure Unique Archaeal Communities in the Anoxic Sediments of Meromictic Lake Kivu

Microbial community structure in methane hydrate-bearing sediments of freshwater Lake Baikal

Diversity of NC10 Bacteria and ANME-2d Archaea in Sediments of Fault Zones at Lake Baikal

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