Geobacter sulfurreducens

Tabares, Marcela; Dulay, Hunter; Reguera, Gemma

doi:10.1016/j.tim.2019.11.004

Cited by 18 publications

(11 citation statements)

References 10 publications

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“…The rod-shaped bacteria could be Geobacter or Enterobacter because they grow in an anaerobic environment, have exoelectrogenic nature, and a rod-shaped structure. 60,61 These microorganisms were reported for hydrogen production in previous studies. 25,62,63 The round-shaped microbial community observed on the anode surface could be Methanococcus, responsible for the conversion of produced CO 2 and H 2 into CH 4..…”

Section: Biofilm Assimilation At Electrodesmentioning

confidence: 94%

Evaluating the use of unassimilated bio‐anode with different exposed surface areas for bioenergy production using solar‐powered microbial electrolysis cell

et al. 2021

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The microbial electrolysis cell (MEC) is an emerging technology for bioenergy production using organic wastewater. Normally, a preassimilated bio-anode is utilized by the MEC to break down the organic content, but the formation and assimilation of microbial community at the anode surface is a time-consuming process. This study utilized a novel unassimilated Ni-foam anode for the first time in solar-powered MEC for bioenergy production. Synthetic dairy manure wastewater (SDMW) was used both as substrate and an inoculum in the solarpowered tubular MEC. The impacts of the exposed surface area of the bio-anode on bioenergy production were evaluated by utilizing two different separation techniques (rate-limited bio-anode -MEC and fully exposed bio-anode -MEC). The former technique achieves a maximum methane production rate of 30.35 ± 0.03 mL/L, 14.2% more than that achieved by the later mentioned technique (26.4 ± 0.05 mL/L). Hydrogen production was approximately 800 ± 5 mm 3 in both experimentations. The maximum generated current in the rate limited bioanode -MEC was 35.5 mA. Scanning electron microscope images confirmed the formation of rod-shaped along with round-shaped microbial communities on the anode surface, and, interestingly, round-shaped bacteria were also grown on the cathode surface. The bioenergy (H 2 and CH 4 ) produced using SDMW within first 13 days of operation, along with the formation of a microbial community, was a significant success in this area and has opened up many research opportunities for producing instant bioenergy from organic waste.

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Section: Biofilm Assimilation At Electrodesmentioning

confidence: 94%

Evaluating the use of unassimilated bio‐anode with different exposed surface areas for bioenergy production using solar‐powered microbial electrolysis cell

et al. 2021

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“…Yalcin et al have also presented a second structure of the conductive nanowires composed of the octaheme cytochrome OmcZ. The reader is directed to recent extensive reviews of the G. sulfurreducens type IV pili, − for an in depth description of their biology and biotechnological potential. Unlike the protein based structure in G. sulfurreducens , a series of discoveries − confirmed that nanowires in S. oneidensis are extensions of the outer membrane and periplasmic inclusions which contain the Mtr outer membrane protein conduit (Figure C).…”

Section: Basic Components Of Extracellular Electron Transfer Pathwaysmentioning

confidence: 99%

Engineering Wired Life: Synthetic Biology for Electroactive Bacteria

et al. 2021

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Electroactive bacteria produce or consume electrical current by moving electrons to and from extracellular acceptors and donors. This specialized process, known as extracellular electron transfer, relies on pathways composed of redox active proteins and biomolecules and has enabled technologies ranging from harvesting energy on the sea floor, to chemical sensing, to carbon capture. Harnessing and controlling extracellular electron transfer pathways using bioengineering and synthetic biology promises to heighten the limits of established technologies and open doors to new possibilities. In this review, we provide an overview of recent advancements in genetic tools for manipulating native electroactive bacteria to control extracellular electron transfer. After reviewing electron transfer pathways in natively electroactive organisms, we examine lessons learned from the introduction of extracellular electron transfer pathways into Escherichia coli. We conclude by presenting challenges to future efforts and give examples of opportunities to bioengineer microbes for electrochemical applications.

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“…Geobacter sulfurreducens has served as a model organism as it is amenable to genetic manipulation and was the first Geobacter species to have its genome fully sequenced (Methé, Nelson et al 2003, Tabares, Dulay et al 2020. G. sulfurreducens can grow on acetate, H 2 , lactate, formate, and CO as electron donors (Lovley, Ueki et al 2011, Speers Allison and Reguera 2012, Geelhoed, Henstra et al 2016, typical electron acceptors are Fe(III)-citrate, Fe(III)phosphate, Co(III), U(VI), S 0 , fumarate, malate (Lovley, Ueki et al 2011) and to some extent also O 2 (microaerobic growth reported in (Lin, Coppi et al 2004, Engel, Vorländer et al 2020).…”

Section: Introductionmentioning

confidence: 99%

Geobacter sulfurreducens metabolism at different donor/acceptor ratios

Frühauf-Wyllie

Holtmann

2022

Preprint

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Geobacter species have great application potential in remediation processes and electrobiotechnology. In all applications, understanding the metabolism will enable target-oriented optimization of the processes. The typical electron donor and carbon source of the Geobacter species is acetate, while fumarate is the usual electron acceptor. Here, we could show that depending on the donor/acceptor ratio in batch cultivation of G. sulfurreducens different product patterns occur. With a donor/acceptor ratio of 1:2.5 malate accumulated as an intermediate product but was metabolized to succinate subsequently. At the end of the cultivation, the ratio of fumarate consumed and succinate produced was approximately 1:1. When fumarate was added in excess, malate accumulated in the fermentation broth without further metabolization. After the addition of acetate to stationary cells, malate concentration decreased immediately and additional succinate was synthesized. Finally, it was shown that also resting cells of G. sulfurreducens could efficiently convert fumarate to malate without an additional electron donor. Overall, it was demonstrated that by altering the donor/acceptor ratio, targeted optimization of the metabolite conversion by G. sulfurreducens can be realized.

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Geobacter sulfurreducens

Cited by 18 publications

References 10 publications

Evaluating the use of unassimilated bio‐anode with different exposed surface areas for bioenergy production using solar‐powered microbial electrolysis cell

Evaluating the use of unassimilated bio‐anode with different exposed surface areas for bioenergy production using solar‐powered microbial electrolysis cell

Engineering Wired Life: Synthetic Biology for Electroactive Bacteria

Geobacter sulfurreducens metabolism at different donor/acceptor ratios

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