Geobacillus toebii subsp. decanicus subsp. nov., a hydrocarbon-degrading, heavy metal resistant bacterium from hot compost

Poli, Annarita; Romano, Ida; Caliendo, Gaetano; Nicolaus, Giancarlo; Orlando, Pierangelo; Falcó, Antonio; Lama, Licia; Gambacorta, Agata; Nicolaus, Barbara

doi:10.2323/jgam.52.223

Cited by 35 publications

(26 citation statements)

References 56 publications

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“…toebii subsp. decanicus' (Poli et al, 2006) await valid publication. The transfer of Bacillus thermantarcticus to the genus Geobacillus, recommended by Zeigler (2005), awaits formal proposal.…”

Section: Introductionmentioning

confidence: 99%

Taxonomic revision of the genus Geobacillus : emendation of Geobacillus , G. stearothermophilus , G. jurassicus , G. toebii , G. thermodenitrificans

Coorevits

Dinsdale

Halket

et al. 2012

International Journal of Systematic and Evolutionary Microbiology

159

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Sixty-two strains of thermophilic aerobic endospore-forming bacteria were subjected to polyphasic taxonomic study including 16S rRNA gene sequence analysis, polar lipid and fatty acid analysis, phenotypic characterization, and DNA-DNA hybridization experiments. Distinct clusters of the species Geobacillus stearothermophilus, Geobacillus thermodenitrificans, Geobacillus toebii and Geobacillus thermoglucosidasius were formed, allowing their descriptions to be emended, and the distinctnesses of the poorly represented species Geobacillus jurassicus, Geobacillus subterraneus and Geobacillus caldoxylosilyticus were confirmed. It is proposed that the name Geobacillus thermoglucosidasius be corrected to G. thermoglucosidans. Bacillus thermantarcticus clustered between Geobacillus species on the basis of 16S rRNA gene sequence analysis, and its transfer to Geobacillus is proposed. The above-mentioned species, together with Geobacillus thermoleovorans and Geobacillus thermocatenulatus, form a monophyletic cluster representing the genus Geobacillus. The distinctness of 'Geobacillus caldoxylosilyticus' was confirmed and it is proposed that it be accommodated, along with Geobacillus tepidamans, in Anoxybacillus. The type strain of Geobacillus debilis was not closely related to any members of Anoxybacillus and Geobacillus, and it is proposed that this species be placed in the new genus Caldibacillus

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Section: Introductionmentioning

confidence: 99%

Taxonomic revision of the genus Geobacillus : emendation of Geobacillus , G. stearothermophilus , G. jurassicus , G. toebii , G. thermodenitrificans

Coorevits

Dinsdale

Halket

et al. 2012

International Journal of Systematic and Evolutionary Microbiology

159

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“…Metal-resistant microorganisms are often presented in contaminated soils [50][51][52][53][54][55]. These bacteria are already well adapted to the local environment and they will likely have a competitive advantage over allochthonous strains if introduced for bioremediation.…”

Section: Discussionmentioning

confidence: 99%

“…Both ChrR and NfsA are cytoplasmic proteins and strain LZ-01 may have a similar mechanism for the reduction of these metals. Desulfovibrio desulfuricans G20 is also known to tolerate Cr(VI), U(VI) and As(V) with a common mechanism for both Cr(VI) and U(VI) reduction [36,49].Metal-resistant microorganisms are often presented in contaminated soils [50][51][52][53][54][55]. These bacteria are already well adapted to the local environment and they will likely have a competitive advantage over allochthonous strains if introduced for bioremediation.…”

mentioning

confidence: 99%

A Novel Subspecies of Staphylococcus aureus from Sediments of Lanzhou Reach of the Yellow River Aerobically Reduces Hexavalent Chromium

Zhang¹,

Krumholz²,

Yu³

et al. 2013

J Bioremed Biodeg

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Background: Lanzhou reach of the Yellow River is contaminated by heavy metals including chromium perennially. The microbial community within the sediment is very active. Yet the study on the bacteria in this distinctive microbial community is still scarce.Results: LZ-01, a Gram-positive hexavalent chromium-reducing bacterium was isolated from the soil sample collected at a petrochemical corporation wastewater discharge site of Lanzhou reach. It was able to aerobically reduce 94.5% of 0.4 mM Cr (VI) to Cr(III) in 120 hours. Cd (II) and NaN 3 treatment both repressed Cr(VI) reduction in LZ-01 and Cr(III) precipitates were detected both on the cell membrane and in the cytoplasm by Transmission Electron Microscopy (TEM) imaging. LZ-01 also demonstrated resistance to 4 mM As (V) and 9 mM U (VI). LZ-01 was closely related to Staphylococcus aureus revealed by 16S rRNA sequence analysis. Comparison of cellular fatty acid components and Vitek phenotype identification provided further evidences that LZ-01 is a novel subspecies of S. aureus. Conclusions:A chromate-reducing bacterium LZ-01 identified as a novel subspecies of S. aureus was isolated from Lanzhou reach of the Yellow River. Cd (II) and NaN 3 treatment and TEM images suggested that Cr(VI) was reduced not only intracellularly but also on the cell membrane. All the results indicate that the isolate has a great potential for bioremediation of Cr (VI)-contaminated environment. Staphylococcus aureus is a facultative Gram-positive coccus-shaped bacterium [27] that is best known for its ability to cause a range of illnesses [27,28]. Several recent studies report that S. aureus is involved in environmental metal remediation [4,29] and S. epidermidis which is closely related to S. aureus is able to anaerobically reduce Cr(VI) to Cr (III) [11]. However, there is no direct evidence that S. aureus can reduce Cr(VI). In this study, we screened the Lanzhou reach river bank soil for Cr(VI) reducers and isolated a novel subspecies of S. aureus strain LZ-01, which reduces Cr(VI) to Cr(III) aerobically. 16S rRNA sequencing and fatty lipids profiling showed that strain LZ-01 is closely related to S. aureus. Comparison of Vitek phenotype identification showed that there were 7 different reactions out of 64 between LZ-01 and type strains of Staphylococcus aureus. LZ-01 reduces Cr(VI) in a highly efficient manner suggesting that it may be useful for Cr(VI) reduction studies in Lanzhou reach. Journal of Bior emediation & Biodegradation Materials and Methods Soil sample collectionThe sampling site was 60 kilometers west of central Lanzhou city and adjacent to the upper reach of the Yellow River. The actual location was close to a petrochemical corporation waste water discharge site. The latitude and longitude of the sampling site is 36°06'N 103°39'E, (Figure 1). The pH of soils at the sampling site ranged from 5.0 to 6.0 with temperature around 18°C at the time of collection. The soil samples were collected from 15 cm below surface horizon and stored in sterile aluminum boxes a...

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“…The pH tolerance of strains was tested at 60 C at different pH values by using buffered NB medium (50 mM MES, HEPES, TAPS and CAPSO) over these pH values: 5.0, 5.5, 6.0, 6.5, 7.5, 8.0, 8.5, 9.0, and 10.0. In order to study the utilization of single carbon sources, the isolates were grown statically using M162 minimal medium (Poli et al, 2006). The organic compounds tested (1%, w/v) were D-glucose, D-lactose, D-maltose, D-fructose, D-galactose, D-xylose, Dmannose, D-trehalose, glycerol, D-cellobiose, sodium acetate and sucrose.…”

Section: Methodsmentioning

confidence: 99%

Geobacillus subterraneus subsp. aromaticivorans subsp. nov., a novel thermophilic and alkaliphilic bacterium isolated from a hot spring in Sırnak, Turkey

Poli

Güven

Romano

et al. 2012

J. Gen. Appl. Microbiol.

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Geobacillus toebii subsp. decanicus subsp. nov., a hydrocarbon-degrading, heavy metal resistant bacterium from hot compost

Cited by 35 publications

References 56 publications

Taxonomic revision of the genus Geobacillus : emendation of Geobacillus , G. stearothermophilus , G. jurassicus , G. toebii , G. thermodenitrificans

Taxonomic revision of the genus Geobacillus : emendation of Geobacillus , G. stearothermophilus , G. jurassicus , G. toebii , G. thermodenitrificans

A Novel Subspecies of Staphylococcus aureus from Sediments of Lanzhou Reach of the Yellow River Aerobically Reduces Hexavalent Chromium

Geobacillus subterraneus subsp. aromaticivorans subsp. nov., a novel thermophilic and alkaliphilic bacterium isolated from a hot spring in Sırnak, Turkey

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