Gentle method for removing metal and restoring function after scanning electron microscopy

Sharma, Rashi; Digaum, Jennefir L.; WEST, H H; Schwarz, Casey M.; Kuebler, Stephen M.

doi:10.1117/1.jmm.20.2.023601

Cited by 3 publications

(3 citation statements)

References 23 publications

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“…painting micro-samples. Moreover, the removal of such layers is done mechanically or using chemicals which can strongly interact and destroy the surface of the sample 26,27,[29][30][31][32][33][34] for further studies.…”

mentioning

confidence: 99%

Conductive cross-section preparation of non-conductive painting micro-samples for SEM analysis

Jaques

Zikmundová

Holas

et al. 2022

Sci Rep

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Scanning electron microscopy (SEM) is a common method for the analysis of painting micro-samples. The high resolution of this technique offers precise surface analysis and can be coupled with an energy-dispersive spectrometer for the acquisition of the elemental composition. For light microscopy and SEM analysis, the painting micro-samples are commonly prepared as cross-sections, where the micro-sample positioned on the side is embedded in a resin. Therefore, the sequence of its layers is exposed after the cross-section is polished. In common cases outside of cultural heritage, a conductive layer is applied on the polished side, but in this field, the measurements are mostly done in low-vacuum SEM (LV-SEM). Although the charging effect is reduced in LV-SEM, it can still occur, and can hardly be prevented even with carbon tape or paint. This work presents two conductive cross-section preparation methods for non-conductive samples, which reduce charging effects without impairing the sample integrity.

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mentioning

confidence: 99%

Conductive cross-section preparation of non-conductive painting micro-samples for SEM analysis

Jaques

Zikmundová

Holas

et al. 2022

Sci Rep

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“…Samples finally transferred to absolute ethanol can then be carefully completely dried using the critical point drying method (CPD) and coated with a thin metal layer 13 , 35 , 36 . Gold, palladium, or platinum are most commonly used 37 , 38 . The surface of such prepared sample is observed in an SEM and images are taken for further analysis.…”

Section: Introductionmentioning

confidence: 99%

The use of scanning electron microscopy and fixation methods to evaluate the interaction of blood with the surfaces of medical devices

Nalezinková,

Loskot,

Myslivcová Fučíková

2024

Sci Rep

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Testing the hemocompatibility of medical devices after their interaction with blood entails the need to evaluate the activation of blood elements and the degree of their coagulation and adhesion to the device surface. One possible way to achieve this is to use scanning electron microscopy (SEM). The aim was to develop a novel SEM-based method to assess the thrombogenic potential of medical devices and their adhesiveness to blood cells. As a part of this task, also find a convenient procedure of efficient and non-destructive sample fixation for SEM while reducing the use of highly toxic substances and shortening the fixation time. A polymeric surgical mesh was exposed to blood so that blood elements adhered to its surface. Such prepared samples were then chemically fixed for a subsequent SEM measurement; a number of fixation procedures were tested to find the optimal one. The fixation results were evaluated from SEM images, and the degree of blood elements’ adhesion was determined from the images using ImageJ software. The best fixation was achieved with the May–Grünwald solution, which is less toxic than chemicals traditionally used. Moreover, manipulation with highly toxic osmium tetroxide can be avoided in the proposed procedure. A convenient methodology for SEM image analysis has been developed too, enabling to quantitatively evaluate the interaction of blood with the surfaces of various medical devices. Our method replaces the subjective assessment of surface coverage with a better-defined procedure, thus offering more precise and reliable results.

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“…Samples nally transferred to absolute ethanol can then be carefully completely dried using the critical point drying method (CPD) and coated with a thin metal layer [13,35,36]. Gold, palladium, or platinum are most commonly used [37,38]. The surface of such prepared sample is observed in an SEM and images are taken for further analysis.…”

Section: Introductionmentioning

confidence: 99%

The use of scanning electron microscopy and fixation methods to evaluate the interaction of blood with the surfaces of medical devices

Nalezinková,

Loskot,

Fučíková

2023

Preprint

View full text Add to dashboard Cite

Testing the hemocompatibility of medical devices after their interaction with blood entails the need to evaluate the activation of blood elements and the degree of their coagulation and adhesion to the device surface. One possible way to achieve this is to use scanning electron microscopy (SEM). The aim was to develop a novel SEM-based method to assess the thrombogenic potential of medical devices and their adhesiveness to blood cells. As a part of this task, also find a convenient procedure of efficient and non-destructive sample fixation for SEM while reducing the use of highly toxic substances and shortening the fixation time. A polymeric surgical mesh was exposed to blood so that blood elements adhered to its surface. Such prepared samples were then chemically fixed for a subsequent SEM measurement; a number of fixation procedures were tested to find the optimal one. The fixation results were evaluated from SEM images, and the degree of blood elements’ adhesion was determined from the images using ImageJ software. The best fixation was achieved with the May-Grünwald solution, which is less toxic than chemicals traditionally used. Moreover, manipulation with highly toxic osmium tetroxide can be avoided in the proposed procedure. A convenient methodology for SEM image analysis has been developed too, enabling to quantitatively evaluate the interaction of blood with the surfaces of various medical devices. Our method replaces the subjective assessment of surface coverage with a better-defined procedure, thus offering more precise and reliable results.

show abstract

Gentle method for removing metal and restoring function after scanning electron microscopy

Cited by 3 publications

References 23 publications

Conductive cross-section preparation of non-conductive painting micro-samples for SEM analysis

Conductive cross-section preparation of non-conductive painting micro-samples for SEM analysis

The use of scanning electron microscopy and fixation methods to evaluate the interaction of blood with the surfaces of medical devices

The use of scanning electron microscopy and fixation methods to evaluate the interaction of blood with the surfaces of medical devices

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