Gentamicin loaded PLA microspheres susceptibility against Staphylococcus aureus and Escherichia coli by Kirby-Bauer and micro-dilution methods

Sabee, M. M. S. Mohd; Awang, Mohd Syafiq; Bustami, Yazmin; Hamid, Zuratul Ain Abdul

doi:10.1063/5.0017438

Cited by 13 publications

(8 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moreover, GMCSNPs and GM–AA–CSNPs showed prolonged and enhanced antimicrobial activity, confirming the higher stability and sustainability of both nano-formulas. The present study is in close agreement with previous studies on the antibacterial activity of gentamicin-loaded polymeric nanoparticles against P. aeruginosa and S. aureus [ 8 , 40 , 41 , 42 , 43 ].…”

Section: Discussionsupporting

confidence: 93%

Gentamicin–Ascorbic Acid Encapsulated in Chitosan Nanoparticles Improved In Vitro Antimicrobial Activity and Minimized Cytotoxicity

et al. 2022

View full text Add to dashboard Cite

Nano-drug delivery is a promising tactic to enhance the activity and minimize the cytotoxicity of antimicrobial drugs. In the current study, chitosan nanoparticles (CSNPs) were used as a carrier for the delivery of gentamicin sulfate (GM) and ascorbic acid (AA). The particles were synthesized by ionotropic gelation method and characterized by FT-IR, Zeta potential, and transmission electron microscope imaging. The obtained particles were evaluated for their in vitro antimicrobial activity and cytotoxicity. The prepared particles (GM–AA–CSNPs) under the optimal condition of 4:1:1 of chitosan to drug ratio showed encapsulation efficiency and loading capacities of 89% and 22%, respectively. Regarding biological activities, GM–AA–CSNPs showed a lower minimum inhibitory concentration (MIC) than free gentamicin sulfate and GMCSNPs mixture without presenting cytotoxicity against normal cells (HSF). Moreover, the GM–AA–CSNPs did not exhibit hemolytic activity. These results highlight that the GM–AA–CSNPs are confirmed as a hopeful formula for future investigations on the development of antimicrobial preparations.

show abstract

Section: Discussionsupporting

confidence: 93%

Gentamicin–Ascorbic Acid Encapsulated in Chitosan Nanoparticles Improved In Vitro Antimicrobial Activity and Minimized Cytotoxicity

et al. 2022

View full text Add to dashboard Cite

show abstract

“…A modified Kirby–Bauer agar diffusion assay [ 24 ] was performed to determine the zone of inhibition (ZOI) of the 5% ( w/w ) CPX-loaded TPU and non-loaded TPU extruded tubes against S. aureus ATCC 25923. S. aureus was cultured to mid-logarithmic growth phase, in TSB at 37 °C and 120 rpm and diluted to 1 × 10 6 CFU/mL.…”

Section: Methodsmentioning

confidence: 99%

Coupling Additive Manufacturing with Hot Melt Extrusion Technologies to Validate a Ventilator-Associated Pneumonia Mouse Model

et al. 2021

View full text Add to dashboard Cite

Additive manufacturing is widely used to produce highly complex structures. Moreover, this technology has proven its superiority in producing tools which can be used in different applications. We designed and produced an extrusion nozzle that allowed us to hot melt extrude drug-loaded tubes. The tubes were an essential part of a new mouse ventilator-associated pneumonia (VAP) model. Ciprofloxacin (CPX) was selected for its expected activity against the pathogen Staphylococcus aureus and ease of incorporation into thermoplastic polyurethane (TPU). TPU was selected as the carrier polymer for its biocompatibility and use in a variety of medical devices such as tubing and catheters. The effect of loading CPX within the TPU polymeric matrix and the physicochemical properties of the produced tubes were investigated. CPX showed good thermal stability and in vitro activity in preventing S. aureus biofilm formation after loading within the tube’s polymeric matrix. Moreover, the produced tubes showed anti-infective efficacy in vivo. The produced tubes, which were extruded via our novel nozzle, were vital for the validation of our mouse VAP model. This model can be adopted to investigate other antibacterial and antibiofilm compounds incorporated in polymeric tubes using hot melt extrusion.

show abstract

“…The test protocols were mainly derived from a method previously reported with some adjustments. 14 A concentration of 1 × 10 8 CFU mL −1 for both bacterial strain cultures were prepared and spread on the dried surface of a MHA plate by using a sterile cotton swab. The diluted bacterial solution was swabbed uniformly on the agar surface by repeating twice and rotating approximately 60° each time.…”

Section: Methodsmentioning

confidence: 99%

“…This method was used to determine the value of minimum inhibitory concentration (MIC). 14,15 Serial dilutions were performed from a starting concentration of 1-2 mg mL À1 of uncapped AgNPs and AuNPs as well as CS-stabilized AgNPs and AuNPs by using MHB as a diluent. Each dilution reduced the concentration by two folds.…”

Section: Antibacterial Assaysmentioning

confidence: 99%

Roles of chitosan in synthesis, antibacterial and anti-biofilm properties of bionano silver and gold

et al. 2022

View full text Add to dashboard Cite

show abstract

Gentamicin loaded PLA microspheres susceptibility against Staphylococcus aureus and Escherichia coli by Kirby-Bauer and micro-dilution methods

Cited by 13 publications

References 12 publications

Gentamicin–Ascorbic Acid Encapsulated in Chitosan Nanoparticles Improved In Vitro Antimicrobial Activity and Minimized Cytotoxicity

Gentamicin–Ascorbic Acid Encapsulated in Chitosan Nanoparticles Improved In Vitro Antimicrobial Activity and Minimized Cytotoxicity

Coupling Additive Manufacturing with Hot Melt Extrusion Technologies to Validate a Ventilator-Associated Pneumonia Mouse Model

Roles of chitosan in synthesis, antibacterial and anti-biofilm properties of bionano silver and gold

Contact Info

Product

Resources

About