Gentamicin encapsulated within a biopolymer for the treatment of <i>Staphylococcus aureus</i> and <i>Escherichia coli</i> infected skin ulcers

Gemeinder, José Lúcio Pádua; Barros, Natan Roberto de; Pegorin, Giovana Sant’Ana; Singulani, Júnya de Lacorte; Borges, Felipe Azevedo; Arco, Marina Constante Gabriel Del; Mendes-Giannini, María José Soares; Almeida, Ana Marisa Fusco; Salvador, Sérgio Luiz de Souza; Herculano, Rondinelli Donizetti

doi:10.1080/09205063.2020.1817667

Cited by 34 publications

(17 citation statements)

References 50 publications

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“…These results suggest that 10 cm (diameter) GF2 and GF3 films will release up to 21 mg GS in 24 h. Previous studies on GS-based wound dressing materials suggest that 0.1% GS is effective for wound infections [ 14 ]. Moreover, topical delivery of 0.1% GS is considered safe for avoiding the toxic effects (ototoxicity and nephrotoxicity) associated with systematic GS administration [ 41 , 51 ].…”

Section: Resultsmentioning

confidence: 99%

Development and Characterization of Gentamicin-Loaded Arabinoxylan-Sodium Alginate Films as Antibacterial Wound Dressing

Alzarea

Alruwaili

Ahmad

et al. 2022

IJMS

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Biopolymer-based antibacterial films are attractive materials for wound dressing application because they possess chemical, mechanical, exudate absorption, drug delivery, antibacterial, and biocompatible properties required to support wound healing. Herein, we fabricated and characterized films composed of arabinoxylan (AX) and sodium alginate (SA) loaded with gentamicin sulfate (GS) for application as a wound dressing. The FTIR, XRD, and thermal analyses show that AX, SA, and GS interacted through hydrogen bonding and were thermally stable. The AXSA film displays desirable wound dressing characteristics: transparency, uniform thickness, smooth surface morphology, tensile strength similar to human skin, mild water/exudate uptake capacity, water transmission rate suitable for wound dressing, and excellent cytocompatibility. In Franz diffusion release studies, >80% GS was released from AXSA films in two phases in 24 h following the Fickian diffusion mechanism. In disk diffusion assay, the AXSA films demonstrated excellent antibacterial effect against E.coli, S. aureus, and P. aeruginosa. Overall, the findings suggest that GS-loaded AXSA films hold potential for further development as antibacterial wound dressing material.

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Section: Resultsmentioning

confidence: 99%

Development and Characterization of Gentamicin-Loaded Arabinoxylan-Sodium Alginate Films as Antibacterial Wound Dressing

Alzarea

Alruwaili

Ahmad

et al. 2022

IJMS

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“…A promising alternative that has been studied is the use of natural rubber latex (NRL) as a carrier for NPs and bioactive compounds [22][23][24][25][26][27][28][29]. NRL is a colloidal system containing 50% water, 4-5% non-rubber (proteins, lipids and carbohydrates) and 30-45% rubber particles (cis-1,4-polyisoprene) [29].…”

Section: Introductionmentioning

confidence: 99%

Silver nanoparticles effect on drug release of metronidazole in natural rubber latex dressing

et al. 2021

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Natural rubber latex (NRL) from Hevea brasiliensis has shown great potential for dermal applications due to its angiogenesis capacity and biocompatibility. Metronidazole (MET) is a synthetic antibiotic used to treat various infections. However, this drug reports dangerous effects when high concentrations are administered. In this study, we used silver nanoparticles (AgNPs) in order to minimize these toxicological effects. For this, the membranes were characterized by physicochemical, molecular modeling, in vitro release and hemocompatibility assays. In the release assays, 14.25% of the MET and 27.28% of the AgNP + MET complex were released simultaneously, indicating that these nanoparticles work as drug carriers. Molecular modeling simulation helped to explain the in vitro release results, showing that AgNPs can interact more efficiently with MET molecules. Moreover, similarities in reactivity between NRL and MET suggested that some drug molecules may remain in the matrix during the release process. The membranes did not present significant hemolytic activity after 24 h of incubation. These results demonstrated that the NRL + AgNP + MET membrane can be used as a dressing in the treatment of infectious processes.

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“…75,76 In addition, the 3D IBF particles were dispersed in the form of crystals on the membranes, as reported in other works, which indicates that significant amount of the pharmaceutical assets can be positioned along the membrane surface. 62,77,78 The presence of IBF on the membrane surface suggests that the drug release can occur very quickly in the solutions, giving rise to the burst release effect, which consists of the almost spontaneous release of some fraction of the compound to the external system. Previous studies have also reported that the drugs, which have crystalline structure on the NRL membranes surfaces, have influenced the burst release.…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Ibuprofen-loaded biocompatible latex membrane for drug release: Characterization and molecular modeling

Lima

Pegorin

Miranda

et al. 2021

Journal of Applied Biomaterials & Functional Materials

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The incorporation of drugs and bioactive compounds in the natural rubber latex (NRL) matrix has been an alternative for the development of transdermal release membranes. Ibuprofen (IBF) is known to be used to treat inflammatory diseases, but when administered orally, high concentrations can cause some adverse problems. In this work, the incorporation of IBF in the NRL membranes was evaluated by physical-chemical, in vitro permeation, hemocompatibility and molecular modeling assays. In addition, the in vitro release profile of IBF in acid and basic media was analyzed during 96 h. The IBF-NRL membrane exhibited the absence of intermolecular bonding that could hinder drug release and presented compatible mechanical properties for applications as a cutaneous adhesive (0.58 and 1.12 MPa to Young’s modulus and rupture tension, respectively). The IBF-NRL system did not present a significant hemolysis degree (1.67%) within 24 h. The release test indicated that in the first hours of the study, 48.5% IBF was released at basic pH and 22.5% at acidic pH, which is characteristic of a burst effect. Then, a stable release profile was observed until the end of the assay, with total IBF release of 60% in alkaline medium and 50% in acidic medium. The drug permeation results indicated that the IBF-NRL membranes can be used for the local skin treatment with permeation of 3.11% of IBF. Dynamic Molecular simulations indicated a pronounced electric dipole in the ionized form of IBF, which suggests a more effective interaction with water, explaining the efficient drug release in alkaline solutions. In general, the results demonstrate that the IBF-NRL membrane has great potential for a new adhesive that can be used for the treatment of inflammatory processes and injuries.

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Gentamicin encapsulated within a biopolymer for the treatment of Staphylococcus aureus and Escherichia coli infected skin ulcers

Cited by 34 publications

References 50 publications

Development and Characterization of Gentamicin-Loaded Arabinoxylan-Sodium Alginate Films as Antibacterial Wound Dressing

Development and Characterization of Gentamicin-Loaded Arabinoxylan-Sodium Alginate Films as Antibacterial Wound Dressing

Silver nanoparticles effect on drug release of metronidazole in natural rubber latex dressing

Ibuprofen-loaded biocompatible latex membrane for drug release: Characterization and molecular modeling

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