Gentamicin administration on the stapes footplate causes greater hearing loss and vestibulotoxicity than round window administration in guinea pigs

King, E. B.; Salt, Alec N.; Kel, Gordana; Eastwood, Hayden; O’Leary, Stephen

doi:10.1016/j.heares.2013.07.013

Cited by 52 publications

(31 citation statements)

References 40 publications

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“…Different drugs had different results [15,16] . It was reported that application of gentamicin to oval window caused more vestibulotoxicity and hearing loss in Gunea pigs [17] . This was supposed to be due to more absorption of gentamicin through the oval window.…”

Section: Discussionmentioning

confidence: 98%

Selective Window Application of Gentamicin Dexamethasone in Meniere's Disease

Ardıç

Tümkaya²,

Aykal³

et al. 2017

Int Adv Otol

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OBJECTIVE:The purpose of the study is to prevent hearing loss when using intratympanic (IT) gentamicin for intractable Meniere's disease. MATERIALS and METHODS:It is a retrospective case review study. Twenty five patients who had definite Meniere's disease and had either selective window application or weekly IT gentamicin were included into the study. First group (selective) had dexamethasone on the round window and gentamicin on oval window during exploratory tympanotomy procedure. The second group had IT gentamicin at weekly intervals. The degree of caloric weakness (CW), average hearing level in low pitch (HLP) (250, 500, 1000, 2000 Hz) and high pitch (HHP) (4000, 6000, 8000 Hz) were compared before and after treatment. The need for further treatment was noted. RESULTS:In the first group, the average HLP was increased from 51.6±7dB to 52.2±5.6 dB. The average HHP was increased 41.96±20.2 dB to 47.2±18.3 dB after treatment. The CW changed from 37.6±23.9 % to 54.6±30.6 %. In the second group, the average HLP was increased from 56.3±10.5 dB to 61.65±18.3 dB. The average HHP was increased 59.05±17.4 dB to 69.4±21.98 dB after treatment. The CW changed from 45.8±22.3% to 71.53±29.63 %. Both methods had statisticaly significant increase in caloric weakness. But only IT gentamicin led a significant hearing loss in HHP. CONCLUSION:The use of dexamethasone and gentamycin via different windows in the middle ear is safe and effective method for Meniere's disease in the short term. Application of dexamethasone protects not only the hearing cells but vestibular cells also.

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Section: Discussionmentioning

confidence: 98%

Selective Window Application of Gentamicin Dexamethasone in Meniere's Disease

Ardıç

Tümkaya²,

Aykal³

et al. 2017

Int Adv Otol

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“…Also, inhibition of steroidogenesis by gentamicin might be due to increasing tumor necrosis factor alpha (TNF-α) gene expression as agreed by Zager et al [35] who demonstrated that TNF-α gene expression was doubled after gentamicin administration. The increased (TNF-α) gene inhibits steroidogenesis in Leydig cells at the transcriptional level of steroidogenic enzymes [36,37]. In rodents, Leydig cells synthesize oestrogen in adults; moreover, it has been shown that germ cells (especially spermatid) from adult male rat account for half of the testicular source of oestrogens through aromatization [38].…”

Section: Discussionmentioning

confidence: 99%

Effect of Stem Cell Therapy on Gentamicin Induced Testicular Dysfunction in Rats

Sm¹,

Nm²,

Sf³

et al. 2017

J Health Med Inform

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Infertility is a worldwide problem affecting couples that have unprotected intercourse, so the current study investigated the possible ameliorative effect of intra testicular stem cell injection in rats subjected to gentamicin induced testicular degeneration. For this study 100 male rats were randomly allocated into 4 groups, namely, the control group (group I), gentamicin group (group II), stem cell group (group III) and gentamicin and stem cell group (group IV). Gentamicin was administered daily for 2 weeks (80 mg/kg) to groups (II and IV). Stem cells were injected intra testicular into rete testis once by the end of gentamicin administration in group (IV) or after I/P injection of saline in group III. Blood and testicular samples were collected by the end of weeks 2, 6 and 10 from group (I) and (II), and by the end of weeks 6 and 10 from group (III and IV). The obtained results showed a significant decreases in serum testosterone, testicular testosterone and estradiol, sperm counts, sperm viability and an increase in % of fragmented DNA (indicating degenerative and necrotic changes) in testicular tissue of group (II) compared to group I , III and IV. Mesenchymal stem cells administration significantly improved all altered testicular parameters, where values of group IV (except testicular estradiol) were not significantly different from those of control group (I). Conclusively, mesenchymal stem cells proved to be beneficial in rats subjected to gentamicin induced testicular dysfunctions.

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“…Within the middle ear, Lutrol F127 will not be in direct contact with the perilymph (with diffusion through round window membrane, and also the stapes as has recently been demonstrated by O'leary and co-workers). 29,30 Therefore, the concentration gradient will be lower and the dissolution of the polymer will be lower too. In addition, as the perilymph effectively does not flow, which is a very different situation to the rotating fluid on an orbital shaker, it is believed that the current system when injected in vivo could be more sustained.…”

Section: Discussionmentioning

confidence: 99%

Inkjet‐Printed Alginate Microspheres as Additional Drug Carriers for Injectable Hydrogels

et al. 2016

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Local delivery of bioactive molecules to the inner ear via diffusion through the round window membrane is becoming an attractive approach to treat sensorineural hearing loss compared to systemic drug administration. Pluronics R (Lutrol F127) are a class of thermosensitive hydrogels that remain liquid prior to injection and rapidly gel under physiological conditions. They are, however, limited to short-term drug release due to rapid hydrolysis in aqueous solution. Therefore, the aim of this study was to investigate an approach, using an ink-jet printing system, to sustain the drug release by incorporating hydrogel microspheres within Lutrol F127. Various concentrations of Lutrol F127 and calcium chloride (CaCl 2 ) were examined by rheology to determine the optimum combinations to use for injection and then blended with inkjet-printed alginate microspheres. Drug release (FITC-Dextran) from Lutrol F127 alone reached completion in less than 24 h. Release from alginate spheres alone showed a burst release and reached 100% in 6 h. Interestingly, the incorporation of microspheres within Lutrol F127 allowed a more sustained release profile and a slower burst release. By varying the quantity of microspheres, concentration of alginate, or ionic cross-linking ratio, the release profile can be adjusted to suit the desired application. C

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Gentamicin administration on the stapes footplate causes greater hearing loss and vestibulotoxicity than round window administration in guinea pigs

Cited by 52 publications

References 40 publications

Selective Window Application of Gentamicin Dexamethasone in Meniere's Disease

Selective Window Application of Gentamicin Dexamethasone in Meniere's Disease

Effect of Stem Cell Therapy on Gentamicin Induced Testicular Dysfunction in Rats

Inkjet‐Printed Alginate Microspheres as Additional Drug Carriers for Injectable Hydrogels

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