Gentamicin

Oden, Edwin M.; Wagman, G. H.; Weinstein, Marvin

doi:10.1016/b978-0-12-403502-7.50027-2

Cited by 5 publications

(3 citation statements)

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“…Antibiotic potencies were determined by means of a disk plate assay with Staphylococcus aureus ATCC 6538P as the test organism. The physical conditions of the assay are similar to those described for gentamicin (1). After separation of components as will be described, the verdamicin standard was established.…”

Section: Ementioning

confidence: 99%

Verdamicin, a New Broad Spectrum Aminoglycoside Antibiotic

Weinstein¹,

Wagman²,

Marquez³

et al. 1975

Antimicrob Agents Chemother

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Section: Ementioning

confidence: 99%

Verdamicin, a New Broad Spectrum Aminoglycoside Antibiotic

Weinstein¹,

Wagman²,

Marquez³

et al. 1975

Antimicrob Agents Chemother

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“…Gentamicin, the first isolated antibiotic from the Micromonospora genera with therapeutic importance, has its potency microbiologically determined through the agar diffusion method (Oden, 1972;Campos, 1982), employing 2 x 2 or 5 x 1 designs, according to Brazilian (Farmacopéia Brasileira, 1988) and American pharmacopoeias (United States Pharmacopeia, 2006), respectively.…”

Section: Introductionmentioning

confidence: 99%

Comparison of three experimental designs employed in gentamicin microbiological assay through agar diffusion

Lourenço

Pinto

2009

Braz. J. Pharm. Sci.

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Gentamicin is a broad-spectrum antibiotic complex produced by actinomycetes belonging to Micromonospora genus and classified among aminoglycoside antibiotics, used in the treatment of serious infections derived from Gram-negative microorganisms. Alterations of their antimicrobial activity not shown in chemical assays can be evaluated through microbiological assays. The aim of this work was to compare 5 x 1, 2 x 2 and 3 x 1 experimental designs, evaluating validation parameters of specificity, linearity, range, precision, and accuracy for each experimental design in different levels of concentration, presentation, and lots. It consisted of 81 assays (in 3 replicas) of gentamicin microbiological dosage. The concentrations of the solutions used were employed in a range from 1.0 μg/mL to 5.0 μg/mL, diluted in phosphate buffer 0.1 M pH 8.0. Antibiotic medium number 11 was used, with Staphyloccocus epidermis (ATCC 12228). 21ml of medium were used as base layer and 4 ml of medium inoculated at 1% were used as surface layer. The dishes were incubated for 18 hours at 37 ± 1 ºC. The three designs employed showed adequate specificity for analysis of dermatological cream and injectable solution containing gentamicin sulphate. They also showed accuracy and linearity in the range evaluated, but not a significant difference concerning precision. The results were compared by means of the determination of the rates of measurement system capacity. The statistical analysis demonstrated that there is no significant difference among the results obtained through 5 x 1, 2 x 2, and 3 x 1, being these equivalent and interchangeable.
A gentamicina é um complexo antibiótico de largo espectro, produzido por actinomicetos do gênero Micromonospora e classificado entre os antibióticos aminoglicosídeos, utilizado no tratamento de infecções graves, devidas a microrganismos Gram-negativos. Alterações da sua atividade antimicrobiana, não demonstradas pelos ensaios químicos, podem ser avaliadas pelos ensaios microbiológicos. O objetivo deste trabalho foi comparar os delineamentos experimentais 5 x 1, 2 x 2 e 3 x 1, avaliando-se os parâmetros de validação de especificidade, linearidade, faixa ou intervalo, precisão e exatidão para cada delineamento experimental em diferentes níveis de concentração, apresentações e lotes. O plano de trabalho constituiu-se na realização de 81 ensaios (em 3 réplicas) de doseamento microbiológico de gentamicina. As concentrações das soluções empregadas foram preparadas numa faixa de 1,0 μg/mL a 5,0 μg/mL, diluídos em tampão fosfato 0,1 M pH 8,0. O meio utilizado foi o meio antibiótico no. 11, com Staphyloccocus epidermidis (ATCC 12228). Empregou-se 21 mL de meio como camada base e 4 mL de meio inoculado à 1% como camada superfície. As placas foram incubadas por 18 horas à 37 ± 1 °C. Os três delineamentos empregados apresentaram especificidade adequada para análise de creme dermatológico e solução injetável contendo sulfato de gentamicina. Também apresentaram exatidão e linearidade no intervalo ava...

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“…13) Antibacterial spectra Minimal inhibitory concentrations of XK-62-2 were determined by agar dilution assay. Fermentation The technique was described in the succeeding paper.1) Isolation and purification To the whole broth harvested from a tank fermentor, oxalic acid was added to precipitate calcium ions and the pH of the whole broth was adjusted to 2.0 with 6 N sulfuric acid.…”

Section: Methodsmentioning

confidence: 99%