Genotypische Variabilität in Ertrags‐ und Qualitätsmerkmalen bei Mohn und Leindotter

Seehuber, Reinhard

doi:10.1002/lipi.19840860501

Cited by 20 publications

(3 citation statements)

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“…Previously, Crowley and Frohlich (1998) found 2% less linolenic acid in C. sativa grown during a dry warm year than in the same variety grown during a normal year. More recently, Vollmann and Eynck (2015) when reviewing the current knowledge about C. sativa, comment the dependence of linolenic acid content on genotype, agronomic treatment or environmental conditions, pointing the fact that, as described in other oilseeds, linolenic acid content was lower in warmer climates than in cooler ones (Seehuber 1984;Rodríguez-Rodríguez et al 2013). These changes should be related to the temperature effect on desaturases activities, indicating that FAD2 and FAD3 proteins from C. sativa are more stable than the ones from other crops.…”

Section: Introductionmentioning

confidence: 99%

Molecular cloning and characterization of the genes encoding a microsomal oleate Δ12 desaturase (CsFAD2) and linoleate Δ15 desaturase (CsFAD3) from Camelina sativa

Rodríguez-Rodríguez

Salas

Venegas-Calerón

et al. 2016

Industrial Crops and Products

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Camelina sativa produces oil that is rich in polyunsaturated linoleic (18:2 Δ9,12) and linolenic (18:3 Δ9,12,15) acids. These fatty acids are obtained by the successive desaturation of oleic (18:1 Δ9) acid, which is catalyzed in the endoplasmic reticulum by two different microsomal desaturases: oleate Δ 12 desaturase (CsFAD2) and linoleate Δ 15 desaturase (CsFAD3). The objective of the present study is to investigate the contribution of these two desaturases to the composition and properties of C. sativa seed oil, and look over the effect that temperature exerts on their activity. Three different copies of both these genes were identified, which when analysed contained three histidine rich motifs (HXCGHX, HRXHH and HVXHH) and six highly conserved transmembrane domains. Comparing their sequences, the CsFAD2 copies accommodated four conservative changes (Glu-36-Asp, Arg-48-His, Val-97-Ala and Ala-177-Pro) and two semi-conservative ones (Val-63-Ile and Leu-249-Met), whereas only one semi-conservative change (Ala-327-Ser) was detected in CsFAD3 but with two extra amino acids (His-147 and Gly-148). The CsFAD2 and CsFAD3 cDNAs were heterologously expressed in Saccharomyces cerevisiae to confirm that they were active enzymes and their dependence on temperature was investigated by growing the recombinant yeast cells at low (22 °C) and optimal (30 °C) temperatures. The conversion of 18:1 Δ9 to 18:2 Δ9,12 by CsFAD2 was slightly better at 30 °C than at 22 °C, and it was also able to desaturate palmitoleic acid (16:1 Δ9) to hexadecadienoic acid (16:2 Δ9,12). By contrast, the conversion driven by CsFAD3 was enhanced 5-fold at 22 °C compared to 30 °C, although it was not able to desaturate 16:2 Δ9,12. The distribution of these microsomal desaturases was also studied in C. sativa, which were expressed most strongly in expanding leaves and developing seeds.

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Section: Introductionmentioning

confidence: 99%

Molecular cloning and characterization of the genes encoding a microsomal oleate Δ12 desaturase (CsFAD2) and linoleate Δ15 desaturase (CsFAD3) from Camelina sativa

Rodríguez-Rodríguez

Salas

Venegas-Calerón

et al. 2016

Industrial Crops and Products

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“…Breeding of camelina, which is a strictly self‐pollinating species, has not been carried out on an intensive level in the past. While its agronomic features have been considered to be quite acceptable, the oil content has been described as being in the low range of 320–440 g/kg seed dry matter (Seehuber 1984, Marquard and Kuhlmann 1986, Vollmann et al. 1996, Zubr 2003).…”

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confidence: 99%

Genetic diversity in camelina germplasm as revealed by seed quality characteristics and RAPD polymorphism

Vollmann¹,

Grausgruber²,

Stift

et al. 2005

Plant Breeding

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Camelina is an alternative oilseed crop species with limited information about the origin and diversity of available germplasm. Therefore, a set of 130 camelina accessions from a world collection was evaluated for oil content, protein content and 1000-seed weight in field experiments grown in three macro-environments in Austria. Based on phenotypic data, accessions were categorized into four groups with different seed characteristics using k-means cluster analysis or principal component extraction. Subsequently, a representative set of 41 accessions was subjected to random amplified polymorphic DNA (RAPD) analysis. Of 24 primers, 15 were polymorphic producing a total of 30 marker loci. Genetic distance estimates between the 41 accessions were calculated, based both on RAPD polymorphism and on seed quality characteristics, and dendrograms were generated for comparison. Similarities were found between the two different clustering approaches, and grouping was partly in agreement with pedigree information or geographic origin. However, as the two estimates of diversity sampled different segments of the genome, i.e. regions coding for seed characteristics or phenotypically neutral genomic regions highlighted by discrete markers, the correlation between the two distance matrices was low.

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“…Two important factors affecting oil yield in camelina plant are seed yield and oil content (Katar et al 2012a). It has been reported that the seed yield, oil rate and, accordingly, the oil yield are largely under the influence of environmental conditions, and the performances of genotypes differ according to changing environmental conditions (Seehuber, 1984;Koncius and Karcauskiene, 2010). Regarding the seed yield in the studies, Katar et al (2012) 2022), They reported that they detected it in the range of 188-3152 kg ha -1 .…”

Section: Resultsmentioning

confidence: 99%

Yield and yield components of some advanced Camelina (Camelina sativa L. CRANTZ) genotypes in Bolu ecological conditions

Arslan,

Yaşar,

İşler

et al. 2024

International Journal of Agriculture Environment and Food Sciences

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The negative effects of global warming are increasing worldwide and this increase is expected to continue. The camelina plant, which can be grown in marginal areas, stands out among other oilseed plants because it is drought-resistant and less costly. In the research, seeds belonging to 8 camelina genotypes, which were prominent in terms of oil rate, seed and oil yield, were used in the trial established in Bolu province, out of a total of 52 genotypes obtained from the seed bank of the United States Department of Agriculture, Agricultural Research Service and Germany. The study was conducted according to the Randomized Complete Block Design in Bolu Abant İzzet Baysal University, Faculty of Agriculture, Research and Application Field in the 2021-2022 production season. In the trait, each plot consisted of six rows with a row length of 3 m, a row spacing of 20 cm, and in row of 5 cm, with three replications. According to the research results; plant height 66.33-71.00 cm, number of branches 3.17-5.07 number plant-1, 1000 seed weight 1.10-1.24 g, seed yield 1095.4-1436.6 kg ha-1, oil content 36.63-40.37%, protein content 23.65-27.22%, oil yield 408.3-559.8 kg ha-1 and protein yield 279.0-391.3 kg ha-1. It was found that values between. According to the results obtained from the study, the K52 genotype in terms of seed yield, the K11 genotype in terms of oil rate and the K52 genotype in terms of protein rate came to the fore.

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Genotypische Variabilität in Ertrags‐ und Qualitätsmerkmalen bei Mohn und Leindotter

Cited by 20 publications

References 2 publications

Molecular cloning and characterization of the genes encoding a microsomal oleate Δ12 desaturase (CsFAD2) and linoleate Δ15 desaturase (CsFAD3) from Camelina sativa

Molecular cloning and characterization of the genes encoding a microsomal oleate Δ12 desaturase (CsFAD2) and linoleate Δ15 desaturase (CsFAD3) from Camelina sativa

Genetic diversity in camelina germplasm as revealed by seed quality characteristics and RAPD polymorphism

Yield and yield components of some advanced Camelina (Camelina sativa L. CRANTZ) genotypes in Bolu ecological conditions

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