Genotyping of tick-borne encephalitis and Kemerovo viruses in taiga ticks collected in the Komi Republic

Карташов, М. Ю.; Микрюкова, Т. П.; Кривошеина, Е. И.; Kuznetsov, Artur; Глушкова, Л И; Korabel'nikov, I V; Егорова, И. Ю.; Терновой, В. А.; Локтев, В Б

doi:10.15789/2220-7619-got-1147

“…Although there has been a certain decrease in TBE incidence in the AR, tick-borne infections are still a vital medical issue in the region. First, a significant share of TBE virus (35 %) in ticks caught in the Komi Republic, a neighboring region to the AR, belongs to the Far East genotype which has high lethality [18]. Secondly, I. persulcatus that prevail in the AR are contaminated not only with TBE virus but also with other pathogens with no specific prevention means against them available at the moment 8 .…”

Section: Results Territorial and Spatial Distribution Of People Bitte...mentioning

confidence: 99%

Aanalyzing risks of incidence of tick-borne encephalitis in areas with different climatic and geographical conditions

Токаревич¹,

Тронин²,

Бузинов³

et al. 2021

Health Risk Analysis

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Ticks are natural reservoirs and vectors of a virus that is an infectious agent of tick-borne encephalitis, a communicable disease with great medical and social significance. Tick-borne encephalitis (TBE) is widely spread in Arkhangelsk region (AR) located in the Arctic zone in Russia where substantial climatic changes are taking place at the moment. Our research involved examining spatial and temporal distribution of numbers of people bitten by ticks, a number of people bitten by ticks per 100 thousand, a number of TBE cases and TBE incidence in districts and settlements in AR. We calculated relative risks of TBE incidence among people bitten by ticks in AR from 1980 to 2019. We analyzed dynamics of indicators showing numbers of people bitten by ticks per 100 thousand and TBE incidence among people living in Arkhangelsk region. The analysis revealed that a number of bitten people grew slowly in 1980–1990, then there was an exponential growth in 1990–2010, and then the trend stabilized in 2010–2019. Dynamics of TBE incidence was completely in line with changes in number of bitten people up to 2014 but there was a substantial drop in TBE incidence after that. Spatial distribution of numbers of bitten people and TBE incidence revealed that average number of bitten people amounted to 25.1 per 100 thousand in the northern districts in 1980–2019 and was statistically significantly lower than in the central and southern districts (р < 0.001). Average long-term incidence was the highest (7.9 per 100 thousand) in the southern districts in comparison with the central (3.0 per 100 thousand; р<0.001) and northern ones (0.7 per 100 thousand; р<0.001). Maximum relative risks of TBE incidence was detected in the southern districts in 1990–1999 (38.8) in comparison with the northern ones. We made an assumption about probable reasons for declining TBE incidence in Arkhangelsk region detected over the last years given the growing numbers of bitten people.

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“…последовательности фрагмента (660 п.н.) гена, кодирующего субъединицу I цитохромоксидазы в митохондриальном геноме клеща [14]. по 5 особей одного пола объединяли в пул.…”

unclassified

Detection of the Genetic Material of the Viruses Tacheng uukuvirus and Sara tick phlebovirus in Taiga Ticks Collected in the Sverdlovsk, Tomsk Regions and Primorsky Territory of Russia and Their Phylogeny

Tupota,

Ternovoi,

Ponomareva

et al. 2023

Problemy Osobo Opasnykh Infektsii

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Extensive spread of tick-borne diseases poses a significant problem for public health and the health of the population living in endemic areas.The aim of the study was to search, analyze genetic material and identify new viral agents of the Phenuiviridae family in taiga ticks collected in Asian regions of Russia using the method of high throughput sequencing.Materials and methods. The study involved 1460 taiga ticks collected in suburban areas of the Tomsk, Yekaterinburg and Primorsky Territory. The genetic material isolated from ticks was sequenced using Illumina technology followed by phylogenetic analysis.Results and discussion. Analysis of the sequencing results made it possible to detect extended nucleotide sequences of the L-gene fragment characteristic of the Phenuiviridae family viruses. We were able to identify 20 nucleotide sequences the length of 250 bp on average in homogenates of Ixodes persulcatus ticks. Eighteen isolates have been identified as members of the genus Uukuvirus and two isolates have been assigned to the genus Phlebovirus, Phenuiviridae family. Phylogenetic analysis has shown that all isolates of the genus Uukuvirus fall under the cluster of Tacheng tick virus 2 belonging to the species Tacheng uukuvirus. They form a separate phylogenetic group which is closely related to two Romanian variants of 2019. Tacheng tick virus 2 was detected in all three surveyed regions of the Asian part of Russia. Two Tomsk isolates of phlebovirus were classified as Sara tick phlebovirus and they clustered with two isolates of phleboviruses from Karelia. Thus, the genetic material of Tacheng tick virus 2 and Sara tick phlebovirus belonging to two genera of the family Phenuiviridae was found in I. persulcatus ticks collected in three geographically different regions of the Asian part of Russia.

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“…Несмотря на некоторое снижение заболеваемости КВЭ в АО, инфекции, передающиеся иксодовыми клещами, не теряют своей актуальности. Вопервых, значительная доля вируса клещевого энцефалита (35 %) в клещах, собранных в соседней с АО Республикой Коми, относятся к дальневосточному генотипу, вызывающему высокую летальность [18]. Во-вторых, в АО I. persulcatus заражены не только вирусом клещевого энцефалита, но и другими патогенами, против которых в настоящее время не разработаны специфические средства профилактики 8 .…”

unclassified

Aanalyzing risks of incidence of tick-borne encephalitis in areas with different climatic and geographical conditions

Токаревич¹,

Тронин²,

Бузинов³

et al. 2021

Health Risk Analysis

0

View full text Add to dashboard Cite

Ticks are natural reservoirs and vectors of a virus that is an infectious agent of tick-borne encephalitis, a communicable disease with great medical and social significance. Tick-borne encephalitis (TBE) is widely spread in Arkhangelsk region (AR) located in the Arctic zone in Russia where substantial climatic changes are taking place at the moment. Our research involved examining spatial and temporal distribution of numbers of people bitten by ticks, a number of people bitten by ticks per 100 thousand, a number of TBE cases and TBE incidence in districts and settlements in AR. We calculated relative risks of TBE incidence among people bitten by ticks in AR from 1980 to 2019. We analyzed dynamics of indicators showing numbers of people bitten by ticks per 100 thousand and TBE incidence among people living in Arkhangelsk region. The analysis revealed that a number of bitten people grew slowly in 1980–1990, then there was an exponential growth in 1990–2010, and then the trend stabilized in 2010–2019. Dynamics of TBE incidence was completely in line with changes in number of bitten people up to 2014 but there was a substantial drop in TBE incidence after that. Spatial distribution of numbers of bitten people and TBE incidence revealed that average number of bitten people amounted to 25.1 per 100 thousand in the northern districts in 1980–2019 and was statistically significantly lower than in the central and southern districts (р < 0.001). Average long-term incidence was the highest (7.9 per 100 thousand) in the southern districts in comparison with the central (3.0 per 100 thousand; р<0.001) and northern ones (0.7 per 100 thousand; р<0.001). Maximum relative risks of TBE incidence was detected in the southern districts in 1990–1999 (38.8) in comparison with the northern ones. We made an assumption about probable reasons for declining TBE incidence in Arkhangelsk region detected over the last years given the growing numbers of bitten people.

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Genotyping of tick-borne encephalitis and Kemerovo viruses in taiga ticks collected in the Komi Republic

Cited by 4 publications

References 14 publications

Aanalyzing risks of incidence of tick-borne encephalitis in areas with different climatic and geographical conditions

Aanalyzing risks of incidence of tick-borne encephalitis in areas with different climatic and geographical conditions

Detection of the Genetic Material of the Viruses Tacheng uukuvirus and Sara tick phlebovirus in Taiga Ticks Collected in the Sverdlovsk, Tomsk Regions and Primorsky Territory of Russia and Their Phylogeny

Aanalyzing risks of incidence of tick-borne encephalitis in areas with different climatic and geographical conditions

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