Genotyping of Thiopurine Methyltransferase Using Pyrosequencing

Okada, Yuko; Nakamura, Katsunori; Wada, M.; Nakamura, Toshio; Tsukamoto, Norifumi; Nojima, Yoshihisa; Horiuchi, Ryuya; Yamamoto, Keiji

doi:10.1248/bpb.28.677

Cited by 14 publications

(7 citation statements)

References 26 publications

(27 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Myelosuppression was defined as occurring when leukocyte counts were reduced to <2.0 × 10 9 /l or when platelet counts were reduced to <1.0 × 10 11 /l. 23 statistical analysis. Genomic DNA was extracted from whole blood using the QIAamp Blood Mini Kit (QIAGEN, Valencia, CA).…”

Section: Discussionmentioning

confidence: 99%

“…9 Genotyping for TPMT*2, *3A, *3B, and *3C alleles was performed using the pyrosequencing method, as previously described. 23 statistical analysis. The correlations among the change in SLEDAI score, age, AZA dose, duration of SLE, SLEDAI score before treatment, and the polymorphisms considered in this study (ITPA, GSTM1, GSTT1, and TPMT) were evaluated using a hierarchical multiple regression analysis.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Pro32Thr Polymorphism of Inosine Triphosphate Pyrophosphatase Gene Predicts Efficacy of Low-Dose Azathioprine for Patients With Systemic Lupus Erythematosus

Okada

Nakamura

Hiromura

et al. 2009

Clin Pharmacol Ther

View full text Add to dashboard Cite

We evaluated the relationship between the efficacy of low-dose azathioprine (AZA) therapy and the inosine triphosphate pyrophosphatase (ITPA) 94C>A (Pro32Thr) polymorphism in patients with systemic lupus erythematosus (SLE). We performed a multiple regression analysis to assess the influence of various factors on the reduction in SLE disease activity index (SLEDAI) scores. The ITPA 94C>A polymorphism had the highest correlation with the change in SLEDAI score (r = 0.354, P = 0.006).

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Pro32Thr Polymorphism of Inosine Triphosphate Pyrophosphatase Gene Predicts Efficacy of Low-Dose Azathioprine for Patients With Systemic Lupus Erythematosus

Okada

Nakamura

Hiromura

et al. 2009

Clin Pharmacol Ther

View full text Add to dashboard Cite

show abstract

“…The Clinical Pharmacogenetics Implementation Consortium offers recommendations regarding drug dosing based on the TPMT genotype (Relling et al, 2011). Until now, various methods have been used for that purpose, including restriction digest analysis (Yates et al, 1997), single-strand conformational polymorphism (Spire-Vayron de la Moureyre et al, 1998), amplification refractory mutation system (Roberts et al, 2004), DHPLC (Schaeffeler et al, 2001), pyrosequencing Okada et al, 2005), and fluorescence hybridization probe assays (Schü tz et al, 2000). Many of these methods are very time consuming, and except for the real time PCR analyses, they require further processing of the amplification product.…”

Section: Discussionmentioning

confidence: 99%

High-Resolution Melting Analysis of theTPMTGene: A Study in the Polish Population

Skrzypczak-Zielińska

Borun

Milanowska

et al. 2013

Genetic Testing and Molecular Biomarkers

View full text Add to dashboard Cite

“…Based upon the relationship between TPMT genotype and phenotype [Okada et al, 2005] the TPMT pharmacogenetics highlights the potential clinical importance of the translation of pharmacogenetics data from bench to bedside . TPMT catalyses the S-methylation of azathioprine, 6-thioguanine and 6-mercaptopurine (6-MP), being these drugs used for the treatment of patients with several disorders such as acute lymphoblastic leukemia, rheumatoid arthritis, systemic lupus erythematosus or other autoimmune/inflammatory diseases.…”

Section: Pharmacogenomicsmentioning

confidence: 99%

Gold Nanoparticle Based Systems in Genetics

Gaspar¹,

Baptista²,

Rueff

2007

CPG

View full text Add to dashboard Cite

Advances in nanoscience are having a significant impact on many scientific fields, boosting the development of a variety of important technologies. The impact of these new technologies is particularly large in biodiagnostics, where a number of nanoparticle-based assays have been introduced for biomolecular detection. The physicochemical malleability and high surface areas of nanoparticle surfaces make them ideal candidates for developing biomarker platforms. Given the variety of strategies afforded through nanoparticle technologies, a significant goal is to tailor nanoparticle surfaces to selectively bind a subset of biomarkers, either for direct detection and characterization or to sequester the target molecules for later study using other available techniques. To date, applications of nanoparticles have largely focused on DNA-or protein-functionalized gold nanoparticles used as the target-specific probes. These unique biophysical properties displayed by gold nanoparticles have huge advantages over conventional detection methods (e.g., molecular fluorophores, microarray technologies). These gold-nanoparticle based systems can then be used for the detection of specific sequences of DNA (pathogen detection, characterization of mutation and/or SNPs) or RNA (without previous retro-transcription and amplification).

show abstract

Genotyping of Thiopurine Methyltransferase Using Pyrosequencing

Cited by 14 publications

References 26 publications

Pro32Thr Polymorphism of Inosine Triphosphate Pyrophosphatase Gene Predicts Efficacy of Low-Dose Azathioprine for Patients With Systemic Lupus Erythematosus

Pro32Thr Polymorphism of Inosine Triphosphate Pyrophosphatase Gene Predicts Efficacy of Low-Dose Azathioprine for Patients With Systemic Lupus Erythematosus

High-Resolution Melting Analysis of theTPMTGene: A Study in the Polish Population

Gold Nanoparticle Based Systems in Genetics

Contact Info

Product

Resources

About