Genotyping of the human platelet antigen systems 1 through 5 by multiplex polymerase chain reaction and ligation‐based typing

Legler, Tobias J.; Köhler, Michael; Mayr, W. R.; Panzer, Simon; Ohto, H.; Fischer, Gottfried

doi:10.1046/j.1537-2995.1996.36596282586.x

Cited by 52 publications

(43 citation statements)

References 21 publications

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“…28,34 GPIIb/IIIa is the final common pathway of activation signals generated by various stimuli and is a key molecule for platelet aggregation, thus being a recent target of antiplatelet therapy. However, because the PlA2 allele (the less frequent allele) is very rare in Japan 35 (our unpublished results indicated that PlA2 type is Ͻ1% in the normal Japanese), we did not include this polymorphism in our analysis.…”

Section: Discussionmentioning

confidence: 99%

Association Between Platelet Glycoprotein Ibα Genotype and Ischemic Cerebrovascular Disease

Sonoda

Murata

Ito

et al. 2000

Stroke

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Background and Purpose-Platelets play pivotal roles in the development of ischemic cerebrovascular disease (CVD).The platelet glycoprotein (GP) Ib/IX/V complex is a receptor for von Willebrand factor, which plays a major role in the initial phase of platelet activation under high shear stress conditions. This study was designed to investigate the association between a genetic variation of this receptor and the prevalence of CVD. Methods-Two hundred patients with ischemic CVD, as confirmed by brain CT and/or MRI, and 317 age-and sex-matched control subjects without clinical evidence of CVD or cardiovascular disease were analyzed for their genotype frequencies of the 145 Thr/Met dimorphism of the ␣-chain of GPIb (GPIb␣). Results-Genotypes with145 Met (T/M and M/M) were more frequently found in the CVD patients (26.5%) than in control subjects (14.2%, Pϭ0.0005). The genotype effect was more obvious in those Ͻ60 years of age or without acquired cardiovascular risk factors. The odds ratio for nonsmoking women Ͻ60 years of age was 10.6 (95% confidence intervals, 2.2 to 51.7). Although the number of patients studied was small (nϭ24), transient ischemic attack showed the highest odds ratio (4.3, Pϭ0.0004), followed by lacunar infarction (ORϭ2.2, Pϭ0.0024) and atherothrombotic infarction (ORϭ1.5, Pϭ0.3143). Logistic regression analysis revealed that the presence of Met-allele was independently associated with CVD. Conclusions-Our study suggests that the platelet GPIb␣ genotype is a genetic risk factor for ischemic CVD.

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Section: Discussionmentioning

confidence: 99%

Association Between Platelet Glycoprotein Ibα Genotype and Ischemic Cerebrovascular Disease

Sonoda

Murata

Ito

et al. 2000

Stroke

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“…Moreover, complete agreement of the results was observed between this new approach including the allele-specific primers for both HPA-la and HPA-lb in a single PCR and the conventional HPA-1 genotyping performed in separate reactions and followed by gel electrophoresis. ternative strategies such as ligase chain reactions [9] of fered adequate specificity. Two groups [7,8] have recently demonstrated that a hot-start technique may enhance both the specificity and the efficiency of sequence-specific PCR for HPA typing.…”

Section: Detection Of the Pcr Products By Elisamentioning

confidence: 99%

“…Legier et al [9] have recently developed a method combin ing a multiplex PCR with ligation-based genotyping which offers genotyping of the platelet antigens HPA-1 to HPA-5 by detection of the reaction products by ELISA. In search of an efficient method for the screening of HPA-1 genotypes, we have evaluated the following approach.…”

Section: Introductionmentioning

confidence: 99%

Genotyping of the Human Platelet Antigen-1 by ELISA Detection of Allele - Specific Amplicons

Müller¹,

Döscher²,

Schunter³

1997

Vox Sanguinis

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Background and objectives: The purpose of the study was to establish a valid and efficient method for genotyping of the human platelet alloantigen-l (HPA-1) in large sample numbers. Materials and methods: Digoxigenin- and fluorescein- labelled allele-specific primers and a biotinylated common primer were included in the hot-start PCR. Amplicons were bound to avidin-coated plates to identify the products by ELISA. Results: This approach reduced the number of PCR analyses for HPA-1 typing by half. PCR products were detected with high sensitivity and good reproducibility (interassay - CV: <8%) in the ELISA. The typing of 100 blood donors with both PCR plus gel electrophoresis and ELISA - PCR showed identical results. Conclusion: This method offers efficient HPA-1 genotyping in large numbers of donors and patients.

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“…2. The allele frequency for 163M is relatively low; for instance, it is 8.6% in the German population (16). In all cases, the PROBE primers were not fully extended, contrary to most PROBE reactions in solution.…”

Section: Resultsmentioning

confidence: 80%

“…ies may be involved (14)(15)(16). DNA fragments containing the polymorphic sites were amplified by the PCR.…”

Section: Resultsmentioning

confidence: 99%

Chip-Based Genotyping by Mass Spectrometry

Tang¹

Integrated Biochips for DNA Analysis

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Silicon chips with immobilized target DNAs were used for accurate genotyping by mass spectrometry. Genomic DNAs were amplified with PCR, and the amplified products were covalently attached to chip wells via Nsuccinimidyl (4-iodoacetyl)aminobenzoate (SIAB) chemistry. Primer annealing, extension, and termination were performed on a 1-l scale directly in the chip wells in parallel. Diagnostic products thus generated were detected in situ by using matrixassisted laser desorption ionization mass spectrometry. This miniaturized method has the potential for accurate, highthroughput, low-cost identification of genetic variations.

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Genotyping of the human platelet antigen systems 1 through 5 by multiplex polymerase chain reaction and ligation‐based typing

Abstract: Multiplex polymerase chain reaction in combination with ligation-based typing allows fast typing of large numbers of platelet donors and screening for critical antigens in pregnant women.

Cited by 52 publications

References 21 publications

Association Between Platelet Glycoprotein Ibα Genotype and Ischemic Cerebrovascular Disease

Association Between Platelet Glycoprotein Ibα Genotype and Ischemic Cerebrovascular Disease

Genotyping of the Human Platelet Antigen-1 by ELISA Detection of Allele - Specific Amplicons

Chip-Based Genotyping by Mass Spectrometry

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