Genotyping of Streptococcus mutans by using arbitrarily primed polymerase chain reaction in children with Down Syndrome

Çoğulu, Dilşah; Sabah, E; Uzel, Ataç; Özkınay, Ferda

doi:10.1016/j.archoralbio.2005.07.008

Cited by 24 publications

(49 citation statements)

References 14 publications

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“…Characterization of oral bacteria by AP-PCR or DNA fingerprinting has become well-accepted (Saarela et al, 1996;Li and Caufield, 1998;Gronroos and Alaluusua, 2000;Li et al, 2001;Toi et al, 2003;Ersin et al, 2004;Klein et al, 2004;Liu et al, 2004;Napimoga et al, 2004;Nascimento et al, 2004;Kamiya et al, 2005;Li et al, 2005;Cogulu et al, 2006;Liu et al, 2007;Hames-Kocabas et al, 2008). Moreover, AP-PCR has been shown to have good discriminating ability for mutans streptococci differentiation (Saarela et al, 1996).…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, AP-PCR has been shown to have good discriminating ability for mutans streptococci differentiation (Saarela et al, 1996). The choice of the primers (OPA 05 and OPA 13) used in the present study was based on previous studies where they were used either independently ( S. mutans 1.33 ± 0.52 S. sobrinus 1.33 ± 0.52 Ersin et al, 2004;Klein et al, 2004;Napimoga et al, 2004;Nascimento et al, 2004;Kamiya et al, 2005;Cogulu et al, 2006;Hames-Kocabas et al, 2008) or in combination (Saarela et al, 1996). The use of two primers for AP-PCR increases the sensitivity and specificity to identify different mutans streptococci amplitypes.…”

Section: Discussionmentioning

confidence: 99%

“…The use of two primers for AP-PCR increases the sensitivity and specificity to identify different mutans streptococci amplitypes. The analysis of AP-PCR fingerprints by sideby-side visual comparison has been used in most studies (Gronroos and Alaluusua, 2000;Li et al, 2001;Toi et al, 2003;Ersin et al, 2004;Cogulu et al, 2006;Liu et al, 2007;HamesKocabas et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

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Horizontal Transmission of Mutans Streptococci in Children

et al. 2009

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It has not been established whether transmission of mutans streptococci occurs between unrelated children older than 4 years of age. The aim of the study was to investigate the possible transmission of mutans streptococci genotypes from child to child in kindergarten. We studied 96 children (ages 5-6 yrs) in three San Francisco Bay Area public schools. Mutans streptococci colonies from each child were isolated from selective culture on Mitis Salivarius Sucrose Bacitracin agar. We used arbitrary primed polymerase chain reactions to determine the mutans streptococci genotypes. Two children (not siblings) in each of the three schools (6%) shared an identical amplitype of S. mutans, unique to each pair. The 19 S. sobrinus amplitypes were found in 12 children, and all were unique to each child. The presence of matching genotypes of S. mutans demonstrates horizontal transmission of this species between unrelated children aged 5-6 years.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Horizontal Transmission of Mutans Streptococci in Children

et al. 2009

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“…Various investigations have pointed to the existence of variations in the ecosystem of the oral cavity among individuals with Down syndrome, which can lead to physiological alterations in the flow and composition of saliva that are important in the microbial colonization of tooth surfaces by such species as S. mutans and S. sobrinus, interfering directly with the initiation of pathological processes such as dental caries [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15].…”

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confidence: 99%

Dental caries experience in relation to salivary findings and molecular identification of S. mutans and S. sobrinus in subjects with Down syndrome

2011

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This study investigated the association between clinical and salivary or molecular parameters in Down syndrome subjects. Sixty individuals (1- to 48-year old) were clinically examined using DMFT/DMFS. Stimulated saliva was collected; salivary flow was calculated (mL/min), buffering capacity was measured using a standard pH tape. In addition, 25 μL of saliva was diluted using 10-fold-dilution method and then placed on Mitis-Salivarius-Bacitracin agar to count colony forming units (CFU/mL) of mutans streptococci. Polymerase chain reaction analysis identified species. Caries indexes were 0.65-13.5 (DMFT) and 0.65-26.0 (DMFS) according to groups. Ninety-four percent of subjects had low flow rate (0.7-1.0 mL/min) and 44% had low buffering capacity (pH < 4). Besides, 60% had more than 1 × 10(6) CFU/mL, 60% had S. mutans, and 41.4% had S. sobrinus. Caries indexes did not significantly correlate with flow rate, buffering capacity, CFU/mL by Pearson's correlation (p > 0.05), and showed no significant association with prevalence of species by Chi-square (p > 0.05). There is no association between clinical picture and salivary or molecular parameters in Down syndrome subjects.

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“…A explicação mais provável para o efeito da idade materna na maior predisposição a gerar um filho com SD é o envelhecimento do gameta feminino 13 . A freqüência da SD é de 1 para 600 a 1000 nascidos vivos 25,30 .…”

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Análise dos parâmetros clínicos periodontais e expressão genética de interferons alfa, gama e genes relacionados em indivíduos portadores de Síndrome de Down com doença periodontal

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Genotyping of Streptococcus mutans by using arbitrarily primed polymerase chain reaction in children with Down Syndrome

Cited by 24 publications

References 14 publications

Horizontal Transmission of Mutans Streptococci in Children

Horizontal Transmission of Mutans Streptococci in Children

Dental caries experience in relation to salivary findings and molecular identification of S. mutans and S. sobrinus in subjects with Down syndrome

Análise dos parâmetros clínicos periodontais e expressão genética de interferons alfa, gama e genes relacionados em indivíduos portadores de Síndrome de Down com doença periodontal

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