Genotyping of Russian isolates of fungal pathogen Trichophyton rubrum, based on simple sequence repeat and single nucleotide polymorphism

Pchelin, Ivan M.; Mochalov, Yuri V.; Azarov, Daniil; Romanyuk, Sofya A.; Чилина, Г А; Выборнова, И В; Богданова, Т В; Zlatogursky, Vasily V.; Apalko, Svetlana V.; Vasilyeva, N. V.; Тараскина, А. Е.

doi:10.1111/myc.13162

Cited by 4 publications

(1 citation statement)

References 62 publications

(121 reference statements)

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“…Two responsible genotypes, however, were detected in each of two episodes, a community of outdoor cats 14) , and in animals and workers at an animal shelter. Although MLMT should be very useful in tracing M. canis infections 15) , it is likely unsuitable for tracking individual cases of T. rubrum infection but can be used to determine its population structure 23,27) .…”

Section: Group K16mentioning

confidence: 99%

Multilocus Microsatellite Analysis of the Molecular Epidemiology of Microsporum canis Isolated in Japan

Mochizuki,

Futatsuya,

Anzawa

et al. 2023

Medical Mycology Journal

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Microsporum canis, a major causative agent of zoonotic dermatophytosis, has become prevalent in Japan. Molecular epidemiological surveys using multilocus microsatellite typing (MLMT), a sensitive genotyping tool for fungi, have been conducted to reveal intraspecies polymorphisms of M. canis. The present study utilized MLMT optimized for Japanese strains of M. canis to analyze epidemic trends of fungal infection. Six individual loci were targeted; namely, MS1, 2, 4, 5, 6, and 7. Analysis of data from 1974 through 2022 identified 416 strains, which were sorted into 60 genotypes by MLMT. The major genotypes showed changes in dominance during this period-changes that may reflect historical increases and decreases in the numbers of patients infected with M. canis patients. The main origins of infection included animal breeders and pet stores, as well as stray cat communities. Forty-nine episodes of familial outbreaks and cohabitant animal infections were recorded, and genotypes responsible for each episode were determined. MLMT analysis is not only a robust tool to understand population structures, but likely the most suitable method for tracking M. canis infections.

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Section: Group K16mentioning

confidence: 99%

Multilocus Microsatellite Analysis of the Molecular Epidemiology of Microsporum canis Isolated in Japan

Mochizuki,

Futatsuya,

Anzawa

et al. 2023

Medical Mycology Journal

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Time and cost‐efficient identification of Trichophyton indotineae

Batvandi

Pchelin

Kiasat

et al. 2022

Mycoses

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Background During the past 5 years, an outbreak of recalcitrant dermatophytosis due to a novel Trichophyton species generally resistant to terbinafine, T. indotineae, has spread out from South Asia to many countries around the World. These isolates cannot be reliably differentiated from other Trichophyton spp. on the basis of morphological traits and the current laboratory diagnostics relies on sequencing of ribosomal DNA ITS region. Objectives In this study, we aimed to introduce two inexpensive and rapid PCR‐based assays for differentiation between T. indotineae and other dermatophytes. Methods The first introduced assay is based on PCR‐restriction fragment length polymorphism (PCR‐RFLP) analysis, involving the amplification of TOP2 sequences and the digestion of PCR products by Cfr13I restriction enzyme. The second assay is proposed as conventional endpoint species‐specific PCR amplification of the C120‐287 intergenic locus. To validate the assays, a total of 191 Trichophyton spp. and 2 Microsporum canis strains with known ITS region sequences were used. From the T. mentagrophytes / T. interdigitale species complex (TMTISC), strains with 18 different ITS genotypes were tested. The sample of TMTISC isolates included 41 T. indotineae strains. Results TOP2 PCR‐RFLP and T. indotineae‐specific PCR were positive with testing on DNA of all 41 T. indotineae isolates and two strains of T. mentagrophytes belonging to ITS Types XIII and XVI, but negative with other species and other TMTISC ITS genotypes (n = 152). Therefore, the specificity of both new assays was 99%. Conclusion The two developed diagnostic assays provide accurate and cost‐effective means of identifying cultured T. indotineae isolates.

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Drug resistance of dermatophytes: do we face the emergence of Indian variants of trichophytosis?

Pchelin¹,

Sergeev²

2023

Klin. dermatol. venerol.

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Genotyping of Russian isolates of fungal pathogen Trichophyton rubrum, based on simple sequence repeat and single nucleotide polymorphism

Cited by 4 publications

References 62 publications

Multilocus Microsatellite Analysis of the Molecular Epidemiology of <i>Microsporum canis</i> Isolated in Japan

Multilocus Microsatellite Analysis of the Molecular Epidemiology of <i>Microsporum canis</i> Isolated in Japan

Time and cost‐efficient identification of Trichophyton indotineae

Drug resistance of dermatophytes: do we face the emergence of Indian variants of trichophytosis?

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