Genotyping of Rickettsial Isolates Circulating in the Crimean Peninsula

Kartashov, M. Yu.; Тихонов, С. Н.; Mikryukova, Tamara P.; Коваленко, И. С.; Терновой, В. А.; Barinova, O. Yu.; Netesov, Sergey V.

doi:10.3103/s0891416818020088

Cited by 4 publications

(6 citation statements)

References 21 publications

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“…Two isolates were identified as R. helvetica with a 100% of similarity level with R. helvetica from I. persulcatus in Novosibirsk (KU310588) and from I. ricinus in our previous study (MH018977; 21 ). One nucleotide sequence was identical with R. aeschlimannii (KU961540; 22 ) originally isolated from Hyalomma marginatum in the Crimean Peninsula (Supplementary File S1 ). To confirm the Rickettsia species identification, the outer membrane protein B ( ompB ) gene fragment (765-bp product) of randomly selected isolates of each species ( R. raoultii , R. helvetica and R. aeschlimannii ) was amplified and sequenced.…”

Section: Resultsmentioning

confidence: 69%

“…To confirm the Rickettsia species identification, the outer membrane protein B ( ompB ) gene fragment (765-bp product) of randomly selected isolates of each species ( R. raoultii , R. helvetica and R. aeschlimannii ) was amplified and sequenced. The nucleotide sequences of the ompB gene fragment were, respectively: (i) identical with R. raoultii from D. reticulatus in Kaliningrad (ON191725) and in Germany (HQ232278; 23 ); (ii) highly similar (99.7%; 763/765) to R. helvetica from I. ricinus in Germany (HQ232251; 23 ) and from I. persulcatus in Russia (KU310591); (iii) R. aeschlimannii from Hyaloma marginatum in the Crimean Peninsula (KU961544; 22 ).…”

Section: Resultsmentioning

confidence: 99%

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Rickettsia species in Dermacentor reticulatus ticks feeding on human skin and clinical manifestations of tick-borne infections after tick bite

Koczwarska,

Pawełczyk,

Dunaj-Małyszko

et al. 2023

Sci Rep

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Dermacentor reticulatus ticks are sporadically removed from human skin and therefore the medical consequences of their feeding are neglected compared to Ixodes ricinus. We investigated the prevalence of pathogens in D. reticulatus removed from human skin and possible clinical manifestations suggestive of tick-borne diseases after a tick bite. A total of 2153 ticks were studied and of these only 34 were D. reticulatus. The mean prevalence of Rickettsia in D. reticulatus was 50.0% and R. raoultii was identified in 82.4% of infected D. reticulatus ticks. We confirmed the first case of R. aeschlimannii infection in D. reticulatus ticks. Among participants bitten by D. reticulatus, 13.3% reported reddening around the tick bite site and flu-like symptoms, including lymphadenopathy and 3.3% reported eschar on the tick site bite. All of the participants with flu-like symptoms after tick removal were bitten by ticks infected with R. raoultii. The results of this study indicate that even though D. reticulatus ticks bite humans sporadically, pathogenic Rickettsia have a remarkably high prevalence in this tick species. We can expect that the incidence of tick-borne lymphadenopathy might increase with the reported expansion of the D. reticulatus into new areas and its growing abundance in Central Europe.

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Section: Resultsmentioning

confidence: 69%

Section: Resultsmentioning

confidence: 99%

Rickettsia species in Dermacentor reticulatus ticks feeding on human skin and clinical manifestations of tick-borne infections after tick bite

Koczwarska,

Pawełczyk,

Dunaj-Małyszko

et al. 2023

Sci Rep

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“…Several tick-borne spotted fever group rickettsiae have been identified in Crimea; however, MSF is the only rickettsiosis that has been diagnosed in the peninsula [11 , 12 , 14] . Previously, we tested R. sanguineus collected from different parts of Crimea and identified DNA of R. conorii subspecies conorii in ticks collected in different locales including Saki district [11 , 12] .…”

Section: Discussionmentioning

confidence: 99%

Use of eschar swab DNA to diagnose Rickettsia conorii subspecies conorii infection in Crimea: A case report

Alieva,

Gafarova,

Bondarenko

et al. 2023

Infectious Medicine

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“…Contemporary acarological studies conducted in the Crimea Peninsula during the last decade indicated the occurrence of diverse tick species in different ecological settings [ 92 , 93 , 94 ]. Nidicolous R. sanguineus is mostly present in the peridomestic environment [ 75 , 76 ].…”

Section: Contemporary Tick Surveillance Efforts In Countries Adjacent...mentioning

confidence: 99%

“…Nidicolous R. sanguineus is mostly present in the peridomestic environment [ 75 , 76 ]. In addition to R. sanguineus infesting dogs and sometimes cats and foxes, Dermacentor marginatus , D. reticulatus , Ixodes ricinus , Hyalomma marginatum , Haemaphysalis punctana and R. bursa were collected in various ecological settings in Crimea [ 92 , 93 , 94 ]. In one location, 28% (n = 57) of R. sanguineus tested positive for R. conorii or R. massiliae [ 92 ].…”

Section: Contemporary Tick Surveillance Efforts In Countries Adjacent...mentioning

confidence: 99%

History and Current Status of Mediterranean Spotted Fever (MSF) in the Crimean Peninsula and Neighboring Regions along the Black Sea Coast

Gafarova,

Eremeeva

2023

Pathogens

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Mediterranean spotted fever (MSF) is a tick-borne rickettsiosis caused by Rickettsia conorii subspecies conorii and transmitted to humans by Rhipicephalus sanguineus ticks. The disease was first discovered in Tunisia in 1910 and was subsequently reported from other Mediterranean countries. The first cases of MSF in the former Soviet Union were detected in 1936 on the Crimean Peninsula. This review summarizes the historic information and main features of MSF in that region and contemporary surveillance and control efforts for this rickettsiosis. Current data pertinent to the epidemiology of the disease, circulation of the ticks and distribution of animal hosts are discussed and compared for each of the countries in the Black Sea basin where MSF occurs.

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Genotyping of Rickettsial Isolates Circulating in the Crimean Peninsula

Cited by 4 publications

References 21 publications

Rickettsia species in Dermacentor reticulatus ticks feeding on human skin and clinical manifestations of tick-borne infections after tick bite

Rickettsia species in Dermacentor reticulatus ticks feeding on human skin and clinical manifestations of tick-borne infections after tick bite

Use of eschar swab DNA to diagnose Rickettsia conorii subspecies conorii infection in Crimea: A case report

History and Current Status of Mediterranean Spotted Fever (MSF) in the Crimean Peninsula and Neighboring Regions along the Black Sea Coast

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