Genotyping of Red Cell Blood Groups by the Liquid Bead Array System (Luminex)

佳奈, 佐々木; 由美, 鈴木; 和美, 伊佐; 隆広, 長部; 直子, 大河内; 史啓, 東; 誠, 内川; 健一, 小笠原; 正博, 佐竹

doi:10.3925/jjtc.63.112

Cited by 2 publications

(4 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Many blood group genotyping platforms have been introduced in blood centres and blood banks, including commercialized kits and in-house panels [8,[11][12][13][14]31]. We enrolled 850 donors in R 1 R 1 / R 2 R 2 group and tested them with Hemo Panel version I assay.…”

Section: Discussionmentioning

confidence: 99%

“…Three S+s+ donors from Reagent red cell donors group were of SS genotype. Sequencing of this region revealed a rare SNV (rs145033200) in the original PCR primer, which caused ADO [11].…”

Section: Ss Phenotypesmentioning

confidence: 99%

“…Because of the emergence of molecular technologies and research into human genomics, the rapid and accurate testing of known single nucleotide variation (SNV) loci can predict blood groups and screen large populations [8]. Different methods have been reported, such as DNA array [9], bead array [10,11], kompetitive allele-specific polymerase chain reaction [12], MassARRAY, [13,14] and massively parallel sequencing (MPS) technology (also called nextgeneration sequencing) [15,16].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The efficacy of ethnic specific blood groups genotyping for routine donor investigation and rare donor identification in Taiwan

et al. 2021

View full text Add to dashboard Cite

Background: Large-scale single nucleotide variation (SNV)-based blood group genotyping assays have been made available for over a decade. Due to differences in ethnic groups, there is much diversity in clinically important blood group antigens and genetic variants. Here, we developed a robust matrix-assisted laser desorption/ ionization time-of-flight (MALDI-TOF)-based blood group genotyping method on MassARRAY system. Study Design andMethods: A total of 1428 donors were enrolled into three groups: (a) reagent red cell donors; (b) rare donor or common antigen-negative donors; and (c) group O, R 1 R 1 /R 2 R 2 donors. Forty-two SNVs were designed for determining nine blood groups, with X/Y chromosome in two multiplex reactions, on MassARRAY 96-well format system. Further targeted sequence analyses were performed by Sanger sequencing.Results: WHO reference reagent (NIBSC code: 11/214) was tested for concordance with the provided genotype results. Among the donors, concordance rate was over 99%. Alleles of important phenotypes such as Mi(a+), Di(a+), and Asian-type DEL and alleles of rare blood groups such as Fy(aÀ), Jk(aÀbÀ) and sÀ were screened.Three types of discrepancies were found. Serologically, the 'N' antigen was expressed on genetically MM with GYP*Mur red blood cells and caused genuine discrepancies (9.5%). Genetically, allele dropout (ADO) was caused by rare SNV in the primer for Ss genotype (2.1%) and partial insertion of RHD genes (0.9%) led to difficulties in predicting phenotypes. Conclusion:Hemo panel module and MassARRAY System in 96-well format showed good performance in terms of large-scale blood group genotyping and phenotype predictions. Implementation of this method is effective for routine blood group genotype screening of donors.

show abstract

Section: Discussionmentioning

confidence: 99%

“…Three S+s+ donors from Reagent red cell donors group were of SS genotype. Sequencing of this region revealed a rare SNV (rs145033200) in the original PCR primer, which caused ADO [11].…”

Section: Ss Phenotypesmentioning

confidence: 99%