Genotyping of red and white fruited strawberry (Fragaria L.) accessions and hybrids based on microsatellite markers and on the genetic diversity in the allergen genes fra a 1 and fra a 3

Kaiser, René; Mageney, Vera; Schwefel, Konrad; Vollmers, Daniela; Kruger, A; Horn, Renate

doi:10.1007/s10722-015-0311-x

Cited by 10 publications

(5 citation statements)

References 67 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Food allergies especially to Rosacea fruits such as apple, peach and strawberry are often associated with birch pollen-related allergies due to cross-reacting IgE antibodies [ 24 ]. About 70% of birch-pollen allergic patients show IgE-mediated food allergies against various fruits and vegetables [ 25 ]. Strawberry allergy mainly affects 30% of population revealing adverse reactions after ingestion of the fruit [ 26 ].…”

Section: Discussionmentioning

confidence: 99%

Effect of the Strawberry Genotype, Cultivation and Processing on the Fra a 1 Allergen Content

et al. 2018

View full text Add to dashboard Cite

Birch pollen allergic patients show cross-reactivity to vegetables and fruits, including strawberries (Fragaria × ananassa). The objective of this study was to quantify the level of the Fra a 1 protein, a Bet v 1-homologous protein in strawberry fruits by a newly developed ELISA, and determine the effect of genotype, cultivation and food processing on the allergen amount. An indirect competitive ELISA using a specific polyclonal anti-Fra a 1.02 antibody was established and revealed high variability in Fra a 1 levels within 20 different genotypes ranging from 0.67 to 3.97 µg/g fresh weight. Mature fruits of red-, white- and yellow-fruited strawberry cultivars showed similar Fra a 1 concentrations. Compared to fresh strawberries, oven and solar-dried fruits contained slightly lower levels due to thermal treatment during processing. SDS-PAGE and Western blot analysis demonstrated degradation of recombinant Fra a 1.02 after prolonged (>10 min) thermal treatment at 99 °C. In conclusion, the genotype strongly determined the Fra a 1 quantity in strawberries and the color of the mature fruits does not relate to the amount of the PR10-protein. Cultivation conditions (organic and conventional farming) do not affect the Fra a 1 level, and seasonal effects were minor.

show abstract

Section: Discussionmentioning

confidence: 99%

Effect of the Strawberry Genotype, Cultivation and Processing on the Fra a 1 Allergen Content

et al. 2018

View full text Add to dashboard Cite

show abstract

“…Anthocyanins are flavonoid compounds present in the ripe colored fruits of Fragaria species. Initially, a role of Fra a 1.02 in the color development in strawberry fruit was proposed, based on the absence of this protein in white species of the Fragaria genus 10 , but this correlation was not further confirmed 30,31 . We observed that silencing Fra a 1.02 in ripe strawberry fruits by more than 92% did not cause a diminution on the content of anthocyanins, but a minor increase was observed when the mean values for control and silencing lines were compared.…”

Section: Fra a 102 Influences The Cell Transport Systemmentioning

confidence: 99%

Down-regulation of Fra a 1.02 in strawberry fruits causes transcriptomic and metabolic changes compatible with an altered defense response

et al. 2021

View full text Add to dashboard Cite

The strawberry Fra a 1 proteins belong to the class 10 Pathogenesis-Related (PR-10) superfamily. In strawberry, a large number of members have been identified, but only a limited number is expressed in the fruits. In this organ, Fra a 1.01 and Fra a 1.02 are the most abundant Fra proteins in the green and red fruits, respectively, however, their function remains unknown. To know the function of Fra a 1.02 we have generated transgenic lines that silence this gene, and performed metabolomics, RNA-Seq, and hormonal assays. Previous studies associated Fra a 1.02 to strawberry fruit color, but the analysis of anthocyanins in the ripe fruits showed no diminution in their content in the silenced lines. Gene ontology (GO) analysis of the genes differentially expressed indicated that oxidation/reduction was the most represented biological process. Redox state was not apparently altered since no changes were found in ascorbic acid and glutathione (GSH) reduced/oxidized ratio, but GSH content was reduced in the silenced fruits. In addition, a number of glutathione-S-transferases (GST) were down-regulated as result of Fra a 1.02-silencing. Another highly represented GO category was transport which included a number of ABC and MATE transporters. Among the regulatory genes differentially expressed WRKY33.1 and WRKY33.2 were down-regulated, which had previously been assigned a role in strawberry plant defense. A reduced expression of the VQ23 gene and a diminished content of the hormones JA, SA, and IAA were also found. These data might indicate that Fra a 1.02 participates in the defense against pathogens in the ripe strawberry fruits.

show abstract

“…On the other hand, in contrast to the usual red pigmentation of strawberry, 'White ananas' is characterized by a white coloration of ripe fruits. This has been attributed to the deficiency of Fra a 1-A proteins, which are critical in pigment biosynthesis [40,41]. The soil was described as loamy and had a pH of 7.55.…”

Section: Experimental Design and Samplingmentioning

confidence: 99%

Insights into the microbiome assembly during different growth stages and storage of strawberry plants

Olimi

Kusstatscher

Wicaksono

et al. 2022

Environmental Microbiome

View full text Add to dashboard Cite

Background Microbiome assembly was identified as an important factor for plant growth and health, but this process is largely unknown, especially for the fruit microbiome. Therefore, we analyzed strawberry plants of two cultivars by focusing on microbiome tracking during the different growth stages and storage using amplicon sequencing, qPCR, and microscopic approaches. Results Strawberry plants carried a highly diverse microbiome, therein the bacterial families Sphingomonadaceae (25%), Pseudomonadaceae (17%), and Burkholderiaceae (11%); and the fungal family Mycosphaerella (45%) were most abundant. All compartments were colonized by high number of bacteria and fungi (107–1010 marker gene copies per g fresh weight), and were characterized by high microbial diversity (6049 and 1501 ASVs); both were higher for the belowground samples than in the phyllosphere. Compartment type was the main driver of microbial diversity, structure, and abundance (bacterial: 45%; fungal: 61%) when compared to the cultivar (1.6%; 2.2%). Microbiome assembly was strongly divided for belowground habitats and the phyllosphere; only a low proportion of the microbiome was transferred from soil via the rhizosphere to the phyllosphere. During fruit development, we observed the highest rates of microbial transfer from leaves and flowers to ripe fruits, where most of the bacteria occured inside the pulp. In postharvest fruits, microbial diversity decreased while the overall abundance increased. Developing postharvest decay caused by Botrytis cinerea decreased the diversity as well, and induced a reduction of potentially beneficial taxa. Conclusion Our findings provide insights into microbiome assembly in strawberry plants and highlight the importance of microbe transfer during fruit development and storage with potential implications for food health and safety.

show abstract

Genotyping of red and white fruited strawberry (Fragaria L.) accessions and hybrids based on microsatellite markers and on the genetic diversity in the allergen genes fra a 1 and fra a 3

Cited by 10 publications

References 67 publications

Effect of the Strawberry Genotype, Cultivation and Processing on the Fra a 1 Allergen Content

Effect of the Strawberry Genotype, Cultivation and Processing on the Fra a 1 Allergen Content

Down-regulation of Fra a 1.02 in strawberry fruits causes transcriptomic and metabolic changes compatible with an altered defense response

Insights into the microbiome assembly during different growth stages and storage of strawberry plants

Contact Info

Product

Resources

About