Genotyping of Mycobacterium tuberculosis isolates from alow-endemic setting in northwestern state of Paraná in Southern Brazil

Noguti, Érika Noda; Leite, Clarice Queico Fujimura; Malaspina, Ana Carolina; Santos, Adolfo Carlos Barreto; Hirata, Rosário Dominguez Crespo; Hirata, Mário Hiroyuki; Mamizuka, Elsa M.; Cardoso, Rosilene Fressatti

doi:10.1590/s0074-02762010000600008

Cited by 27 publications

(27 citation statements)

References 25 publications

(21 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…According to Hunter and Gaston [22] the most allelic diversity observed in the M. tuberculosis isolates studied was in MIRU loci 40 and 26 and moderated polymorphism were found in MIRU loci 2, 10, 16, 20, 23, 27 and 31. These allelic diversities also differs from the obtained in northwestern Paraná by Noguti et al [12] that observed most allelic diversity in MIRU loci 40, 23, 10 and 16 and moderate polymorphism in MIRU loci 26, 20 and 31. Sharma et al [14] working with only 6 loci-MIRU (MIRU 4, 10, 16, 26, 39 and 40) observed the most discriminatory loci, in order of diversity, 26, 10, 16 and 40 in isolates from Kanpur, India.…”

Section: Discussioncontrasting

confidence: 99%

“…The present results are similar to a previous one, carried out in our laboratory, by Noguti et al [12], which had greater variability in SITs, but LAM, H and T were the largest clades in the northwestern Paraná. In Araraquara, São Paulo State, which borders northern Paraná, Mendes et al [13] identified 73% of spoligopatterns in SpolDB4, and LAM, T and H clades were the most prevalent too.…”

Section: Discussionsupporting

confidence: 92%

See 1 more Smart Citation

First Baseline of Circulating Genotypic Lineages of Mycobacterium tuberculosis in Patients from the Brazilian Borders with Argentina and Paraguay

Machado

Marcondes

Leite³

et al. 2014

PLoS ONE

Self Cite

View full text Add to dashboard Cite

BackgroundAt the triple border Brazil/Paraguay/Argentina there is easy mobility from one city to another for economic and tourism activities. This constant and fast population mobility is mainly to visit Iguazu Falls, in the Iguazu River, on the border of the Brazilian state of Paraná and the Argentina. As the incidence of tuberculosis is high in this setting, our study aimed to establish a first baseline of circulating genotypic lineages of Mycobacterium tuberculosis.Methodology/Principal FindingsThis study included 120 patients from 10 cities in southwestern Paraná, Brazil with pulmonary symptoms, from July 2009 to July 2011. Information about sex, age, clinical features and address was collected by reviewing the national tuberculosis notification database. Of these, 96 (80%) isolates were identified as M. tuberculosis and 22 (22.9%) were drug resistant (20, 20.8% INH mono-resistant and 2, 2.1% multidrug-resistant). All isolates were subjected to genotyping by Spoligotyping and MIRU-VNTR typing. The distribution of the isolates analyzed by spoligotyping revealed 30 distinct patterns. The four mainly detected clades were Latin American and Mediterranean (LAM), ill-defined T, Haarlem (H) and S. The MIRU-VNTR showed 85 distinct patterns. Spoligotyping combined to MIRU-VNTR allowed 90 distinct patterns.Conclusions/SignificanceOur study demonstrated that there is significant molecular diversity in circulating M. tuberculosis, with predominance of the LAM and T clades in cities of southwestern Paraná, Brazil, bordering Argentina and Paraguay.

show abstract

Section: Discussioncontrasting

confidence: 99%

Section: Discussionsupporting

confidence: 92%

First Baseline of Circulating Genotypic Lineages of Mycobacterium tuberculosis in Patients from the Brazilian Borders with Argentina and Paraguay

Machado

Marcondes

Leite³

et al. 2014

PLoS ONE

Self Cite

View full text Add to dashboard Cite

show abstract

“…Previous studies analysing Mtb isolates from the state of Rio Grande do Sul in Southern Brazil reported the prevalence of LAM, unknown, H e T, as described in our study; however, the authors analysed a lower number of isolates within a region of low TB prevalence (Noguti et al 2010, Von Groll et al 2010. Interestingly, no Beijing and CAS strains were found in the investigation herein, an observation that is corroborated by a recent report from Ritacco et al 2008.…”

Section: Resultssupporting

confidence: 90%

Spoligotyping of clinical Mycobacterium tuberculosis isolates from the state of Minas Gerais, Brazil

Miranda

Carvalho

Suffys

et al. 2011

Mem. Inst. Oswaldo Cruz

View full text Add to dashboard Cite

We performed spoligotyping on 114 strains of the Mycobacterium tuberculosis (Mtb) complex that had been isolated from patients in Minas Gerais Health Units during 2004. A total of 82/114 (72%) clinical isolates were clustered and 32/114 (28%) were unique. Seven shared types containing nine strains were newly created. A total of nine patterns corresponded to unreported orphan strains, as evaluated against all of the strains recorded in the SITVIT2 proprietary database in the Institut Pasteur de la Guadeloupe. The major clades were composed of isolates that belong to the following genotypes: Latin-America and Mediterranean (63/114, 55.3%) (the ill-defined T superfamily) (12/114, 10.5%), Haarlem (8/114, 7%), X clade (6/114, 5.3%), S clade (3/114, 2.6%) and the East-African Indian and Manu types, each with 1/114 (0.9%) isolates. A considerable number of strains (n = 20, 17.5%) showed patterns that did not fall within any of the previously described major clades. We conclude the bulk of tuberculosis (TB) (92/114, 80.7%) in our location is recent evolutionary strains that belong to the principal genetic groups 2/3. Further studies on epidemiology of TB are required to understand Mtb biodiversity and TB transmission in this region

show abstract

“…The preponderance of these M. tuberculosis families in São Paulo city and Lisbon is probably due to the close relationship between Brazil and Portugal, especially between the two cities. In Brazil, studies carried out in Rio Grande do Sul [20] and in Maringa (PR) [21] corroborate the prevalence of these families. The families LAM and T and also Haarlem are most frequently found in Africa, South America and Central Europe [12].…”

Section: Discussionmentioning

confidence: 87%

Characterization of the genetic diversity of Mycobacterium tuberculosis in São Paulo city, Brazil

Mendes

Melo²,

Santos

et al. 2011

BMC Res Notes

View full text Add to dashboard Cite

BackgroundTuberculosis is a major health problem in São Paulo, Brazil, which is the most populous and one of the most cosmopolitan cities in South America. To characterize the genetic diversity of Mycobacterium tuberculosis in the population of this city, the genotyping techniques of spoligotyping and MIRU were applied to 93 isolates collected in two consecutive years from 93 different tuberculosis patients residing in São Paulo city and attending the Clemente Ferreira Institute (the reference clinic for the treatment of tuberculosis).FindingsSpoligotyping generated 53 different spoligotype patterns. Fifty-one isolates (54.8%) were grouped into 13 spoligotyping clusters. Seventy- two strains (77.4%) showed spoligotypes described in the international databases (SpolDB4, SITVIT), and 21 (22.6%) showed unidentified patterns. The most frequent spoligotype families were Latin American Mediterranean (LAM) (26 isolates), followed by the T family (24 isolates) and Haarlem (H) (11 isolates), which together accounted for 65.4% of all the isolates. These three families represent the major genotypes found in Africa, Central America, South America and Europe. Six Spoligo-International-types (designated SITs by the database) comprised 51.8% (37/72) of all the identified spoligotypes (SIT53, SIT50, SIT42, SIT60, SIT17 and SIT1). Other SITs found in this study indicated the great genetic diversity of M. tuberculosis, reflecting the remarkable ethnic diversity of São Paulo city inhabitants. The MIRU technique was more discriminatory and did not identify any genetic clusters with 100% similarity among the 93 isolates. The allelic analysis showed that MIRU loci 26, 40, 23 and 10 were the most discriminatory. When MIRU and spoligotyping techniques were combined, all isolates grouped in the 13 spoligotyping clusters were separated.ConclusionsOur data indicated the genomic stability of over 50% of spoligotypes identified in São Paulo and the great genetic diversity of M. tuberculosis isolates in the remaining SITs, reflecting the large ethnic mix of the São Paulo city inhabitants. The results also indicated that in this city, M. tuberculosis isolates acquired drug resistance independently of genotype and that resistance was more dependent on the selective pressure of treatment failure and the environmental circumstances of patients.

show abstract

Genotyping of Mycobacterium tuberculosis isolates from alow-endemic setting in northwestern state of Paraná in Southern Brazil

Cited by 27 publications

References 25 publications

First Baseline of Circulating Genotypic Lineages of Mycobacterium tuberculosis in Patients from the Brazilian Borders with Argentina and Paraguay

First Baseline of Circulating Genotypic Lineages of Mycobacterium tuberculosis in Patients from the Brazilian Borders with Argentina and Paraguay

Spoligotyping of clinical Mycobacterium tuberculosis isolates from the state of Minas Gerais, Brazil

Characterization of the genetic diversity of Mycobacterium tuberculosis in São Paulo city, Brazil

Contact Info

Product

Resources

About