Genotyping of Mycobacterium leprae present on Ziehl-Neelsen-stained microscopic slides and in skin biopsy samples from leprosy patients in different geographic regions of Brazil

Fontes, Amanda Nogueira Brum; Gomes, Harrison Magdinier; Araujo, Marcelo Ivens de; Albuquerque, Edson Cláudio Araripe de; Baptista, Ida Maria Foschiani Dias; Moura, Maria Manuela da Fonseca; Rezende, Denise Silva; Pessolani, Maria Cristina Vidal; Lara, Flávio Alves; Pontes, Maria Araci de Andrade; Gonçalves, Heitor de Sá; Lucena‐Silva, Norma; Sarno, Euzenir Nunes; Vissa, Varalakshmi; Brennan, Patrick J.; Suffys, Philip Noël

doi:10.1590/s0074-02762012000900021

Cited by 20 publications

(25 citation statements)

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“…The detection of drug-resistant M. leprae strains has been reported in Brazil (14)(15)(16). Nevertheless, circulating genotypes and resistant strains are not routinely surveyed worldwide (17).…”

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confidence: 99%

Identification of Primary Drug Resistance to Rifampin in Mycobacterium leprae Strains from Leprosy Patients in Amazonas State, Brazil

et al. 2014

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Identification of Primary Drug Resistance to Rifampin in Mycobacterium leprae Strains from Leprosy Patients in Amazonas State, Brazil

et al. 2014

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“…Approximately four million slaves were brought to Brazil between the 17 th and 19 th centuries 26 and leprosy disease was widespread among the slaves. In the state of Ceará, slave labor from Western Africa was used by sugarcane plantations, which was the main activity during the colonial period, as already demonstrated in other genotyping studies 1,27 . Thus, the predominance of SNP type 4 in Ceará is directly related to the Western African slaves.…”

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confidence: 55%

Presence of Mycobacterium leprae genotype 4 in environmental waters in Northeast Brazil

Holanda

Marques

Macedo

et al. 2017

Rev. Soc. Bras. Med. Trop.

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Introduction: This study quantified Mycobacterium leprae bacilli in environmental water samples from five municipalities in the State of Ceará by quantitative polymerase chain reaction (qPCR) and compared the identified genotypes with those obtained from leprosy patient biopsies. Methods: We collected five replicas from each of the 30 selected reservoirs and skin lesion biopsies from 25 new leprosy cases treated at a reference center in Fortaleza, Ceará from 2010 to 2013. The 16S rRNA gene region of M. leprae was amplified by qPCR and a standard curve was created with the pIDTBlue 16SrRNAMlep plasmid. The Juazeiro do Norte water samples and the biopsies were genotyped (single nucleotide polymorphism [SNP] 1 to 4) and the SNP 4 genotypes were subtyped. Results: Of the 149 water samples analyzed, 54.4% were positive for the M. leprae DNA. The M. leprae bacilli copy number ranged from 1.42 × 10 -1 to 1.44 × 10 + 2 . Most biopsies showed SNP type 4 (64%), while all samples from Juazeiro do Norte were SNP type 4, with subtype 4-N appearing at the highest frequency. Conclusions: We suggest that environmental waters containing M. leprae bacilli play an important role in disease transmission, justifying PGL-1 seropositivity in individuals living in areas where there is no reported case, and in leprosy cases individuals who report no previous contact with other case. Therefore, further investigation is needed to clarify disease transmission in this region and to explore the role of the environment. We also suggest that in this area surveillance for leprosy cases should be intensified.

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“…Each sample is represented as having a distinct profile which may be attributed to polymorphic VNTR loci. Fontes et al, 2009Fontes et al, , 2012Sakamuri et al, 2009;Shinde et al, 2009, Kuruwa et al, 2012. Our study has been carried out with the aim of analysing various known genomic markers to elicit epidemiologically relevant information about the transmission of the disease and to compare strain types within the studied population with VNTRs.…”

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confidence: 99%

Genotyping of Mycobacterium leprae strains from a region of high endemic leprosy prevalence in India

Lavania¹,

Jadhav²,

Turankar³

et al. 2015

Infection, Genetics and Evolution

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Genotyping of Mycobacterium leprae present on Ziehl-Neelsen-stained microscopic slides and in skin biopsy samples from leprosy patients in different geographic regions of Brazil

Abstract: We analysed 16 variable number tandem repeats (VNTR) and three single-nucleotide polymorphisms (SNP) in

Cited by 20 publications

References 19 publications

Identification of Primary Drug Resistance to Rifampin in Mycobacterium leprae Strains from Leprosy Patients in Amazonas State, Brazil

Identification of Primary Drug Resistance to Rifampin in Mycobacterium leprae Strains from Leprosy Patients in Amazonas State, Brazil

Presence of Mycobacterium leprae genotype 4 in environmental waters in Northeast Brazil

Genotyping of Mycobacterium leprae strains from a region of high endemic leprosy prevalence in India

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