Genotyping of
            <i>Plasmodium falciparum</i>
            Pyrimethamine Resistance by Matrix-Assisted Laser Desorption-Ionization Time-of-Flight Mass Spectrometry

Marks, Florian; Meyer, Christian G.; Sievertsen, Jürgen; Timmann, Christian; Evans, Jennifer; Horstmann, Rolf D.; May, Jürgen

doi:10.1128/aac.48.2.466-472.2004

Cited by 15 publications

(8 citation statements)

References 21 publications

(29 reference statements)

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“…Such studies have focused on identifying a distinctive configuration of circulating serum proteins that are indicative of a specific pathophysiological state, a so‐called ‘proteomic fingerprint’ (47). Also, assays based on MALDI‐TOF MS allowed the identification of Plasmodium falciparum dihydropteroate synthetase genes associated with pyrimethamine resistance, an antimalarial drug for treatment of malaria in several areas where this disease is endemic (48). On the other hand, novel glycoconjugates related to schistosomiasis were identified in urine of Schistosoma mansoni ‐infected individuals using a combination of glycopeptide separation techniques and in‐depth mass spectrometric analysis (49), and in Trichomonas vaginalis , the ceramide phosphoinositol glycan core released by mild acid that defines molecular domains of the parasite surface glycol‐conjugate lipophosphoglycan with distinct functions in the host immunoinflammatory response was determined by MALDI‐TOF MS (50).…”

Section: Resultsmentioning

confidence: 99%

Structural and immunological characterization of sulphatides: relevance of sulphate moieties in Trypanosoma cruzi glycoconjugates

Acosta

Soprano

Ferrero

et al. 2012

Parasite Immunology

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Summary Sulphoglycosphingolipids, present on the surface of diverse cells, participate in the regulation of various cellular events. However, little is known about the structure and the role of sulphoglycosphingolipids in trypanosomatids. Herein, sulphated dihexosylceramide structures – composed mainly of sphingosine as the long chain base acylated with stearic acid – have been determined for the first time in Trypanosoma cruzi epimastigotes by UV‐MALDI‐TOF‐MS analysis. Interestingly, inhibition ELISA assays using cruzipain as antigen and polyclonal rabbit antibodies specific for cruzipain, the major cysteine proteinase of T. cruzi, or for its C‐terminal domain, have demonstrated (i) that sulphate epitopes are shared between cruzipain and sulphatides of T. cruzi, (ii) that cross‐reactivity maps to the C‐terminal domain and (iii) the existence of other antigenic determinants in the glycolipidic structures. These features provide evidence that sulphate groups are antigenic in sulphate‐containing parasite glycoconjugates. Furthermore, IgG2 antibody levels inversely correlate with disease severity in chronic Chagas disease patients, suggesting that IgG2 antibodies specific for sulphated epitopes might be associated with protective immunity and might be considered as potential surrogates of the course of chronic Chagas disease.

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Section: Resultsmentioning

confidence: 99%

Structural and immunological characterization of sulphatides: relevance of sulphate moieties in Trypanosoma cruzi glycoconjugates

Acosta

Soprano

Ferrero

et al. 2012

Parasite Immunology

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“…Other methods for typing P. falciparum are based on microsatellite (MS) analysis [Su et al, 1998], reverse transcriptase (RT) PCR [Menegon et al, 2000], amplified fragment length polymorphism (AFLP) [Rubio et al, 2001], sequence analysis [Zhu et al, 2002], fluorogenic PCR [Decuypere et al, 2003], minisatellite variant repeat (MVR) mapping [MacLeod, 2004] and matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF MS) [Marks et al, 2004]. These methods have however still limitations in determining multiple concurrent parasite genotypes in single samples in large epidemiological studies.…”

Section: Blockmentioning

confidence: 99%

Spleen enlargement and genetic diversity of Plasmodium falciparum infection in two ethnic groups with different malaria susceptibility in Mali, West Africa

Bereczky

Dolo

Maiga

et al. 2006

Transactions of the Royal Society of Tropical Medicine and Hygi

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The high resistance to malaria in the nomadic Fulani population needs further understanding. The ability to cope with multiclonal Plasmodium falciparum infections was assessed in a cross-sectional survey in the Fulani and the Dogon, their sympatric ethnic group in Mali. The Fulani had lower parasite prevalence and densities and more prominent spleen enlargement. Spleen rates in children aged 2-9 years were 75% in the Fulani and 44% in the Dogon (P<0.001). There was no difference in number of P. falciparum genotypes, defined by merozoite surface protein 2 polymorphism, with mean values of 2.25 and 2.11 (P=0.503) in the Dogon and Fulani, respectively. Spleen rate increased with parasite prevalence, density and number of co-infecting clones in asymptomatic Dogon. Moreover, splenomegaly was increased in individuals with clinical malaria in the Dogon, odds ratio 3.67 (95% CI 1.65-8.15, P=0.003), but not found in the Fulani, 1.36 (95% CI 0.53-3.48, P=0.633). The more susceptible Dogon population thus appear to respond with pronounced spleen enlargement to asymptomatic multiclonal infections and acute disease whereas the Fulani have generally enlarged spleens already functional for protection. The results emphasize the importance of spleen function in protective immunity to the polymorphic malaria parasite.

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“…The first study was designed to look at correlates of immunity during an IPTi study with follow up to 24 months of age, whereas the second was designed to detect exposure to blood stage infection following a single dose of SP. In addition the first study took place in Mozambique (Mayor et al, 2008) where the frequency of resistant alleles to SP was higher than in the second study in Ghana (Marks et al, 2004). It is assumed that children will be exposed to malaria in between doses of IPTi when the drug concentration falls below the required levels for effective prophylaxis and will thus develop immunity.…”

Section: Effects On Immunitymentioning

confidence: 99%

Impact of seasonal malaria chemoprevention of sulphadoxinepyrimethamine plus amodiaquine on molecular markers resistance of Plasmodium falciparum malaria: A review in West Africa

Maiga¹

2016

Clin. Rev. Opinions

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The study discuss the potential impact of seasonal malaria chemoprevention (SMC) on the cases of malaria, anaemia and molecular markers resistance Plasmodium falciparum malaria; and also review the mechanism of action and the effect on immunity of SMC. SMC using an efficacious drug is likely to substantially reduce cases of clinical malaria in high transmission settings. However, an increase of molecular markers could hamper rolling out SMC as a national policy.

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Genotyping of Plasmodium falciparum Pyrimethamine Resistance by Matrix-Assisted Laser Desorption-Ionization Time-of-Flight Mass Spectrometry

Cited by 15 publications

References 21 publications

Structural and immunological characterization of sulphatides: relevance of sulphate moieties in Trypanosoma cruzi glycoconjugates

Structural and immunological characterization of sulphatides: relevance of sulphate moieties in Trypanosoma cruzi glycoconjugates

Spleen enlargement and genetic diversity of Plasmodium falciparum infection in two ethnic groups with different malaria susceptibility in Mali, West Africa

Impact of seasonal malaria chemoprevention of sulphadoxinepyrimethamine plus amodiaquine on molecular markers resistance of Plasmodium falciparum malaria: A review in West Africa

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