Genotyping ofChlamydia trachomatisin rectal and pharyngeal specimens: identification of LGV genotypes in Finland

Abstract

Our experience suggests that genotyping C trachomatis by pmpH PCR can be introduced as a function of a diagnostic laboratory already using NAAT for detection of C trachomatis. The data show that LGV infections occur also in Finland. LGV should be taken into account when considering treatment and management of rectal C trachomatis infections.

Chlamydia trachomatis infection prevalence and serovar distribution in a high-density urban area in the north of Italy

“…As underlined by our findings, CT serovars other than L2 can be found in rectal and pharyngeal mucosa with different rates on the basis of the population enrolled (Korhonen et al, 2012;Mejuto et al, 2013;Labiran et al, 2015). In this context, the correct identification of CT serovar has a crucial role in establishing adequate antibiotic treatment, since, in a case of L1-L3 serovar detection, an LGV regimen should be started (Mohrmann et al, 2014;Workowski & Bolan, 2015).…”

“…The particular tropism of L2 serovar for rectal mucosa and the sporadic cases of urethral LGV highlighted the importance of performing CT NAAT testing on extra-genital sample and of carrying out CT genotyping on this kind of specimens (Korhonen et al, 2012;Mejuto et al, 2013;Mohrmann et al, 2014).…”

“…LGV in extra-genital samples of high-risk population) is crucial for the appropriate management of patients in terms of antibiotic treatment and prevention of complications (Korhonen et al, 2012;Mejuto et al, 2013).…”

“…Since commercial NAATs for CT detection do not allow differentiation between serovars or the identification of LGV, many laboratories have set up in-house methods, even in the absence of US Food and Drug Administration (FDA) clearance or Conformit e Europ eene (CE) marking (Klint et al, 2006;Jalal et al, 2007;Stevens et al, 2010;Korhonen et al, 2012). As reviewed by Pedersen et al (2009) and Xia & Xiong (2014), many molecular methods, mainly based on omp1 or pmpH gene amplification and characterization, are nowadays available, showing different performances in terms of sensitivity, specificity and ability in the detection of mixed infections.…”

“…Finally, CT genotyping has a critical role in the choice of appropriate antibiotic treatment in cases where LGV is suspected, because when L1-L3 serovars are detected, patients should receive a 3 week therapy regimen (doxycycline 100 mg orally twice a day for 21 days), instead of a 1 week approach (Korhonen et al, 2012;Workowski & Bolan, 2015).…”