Genotyping of four genetic polymorphisms in the CYP1A2 gene in the Egyptian population

Abstract

Aims The goal of this study was to determine the frequencies of CYP1A2 * 1C , * 1D , * 1E and * 1F variants in the Egyptian population and compare frequencies with other populations. Methods Genotyping was performed in a total of 212 unrelated Egyptian subjects using polymerase chain reaction-restriction fragment length polymorphism assay. Results The frequencies of CYP1A2 * 1C , * 1D , * 1E and * 1F variants in the Egyptian population were 0.07, 0.40, 0.03 and 0.68, respectively. The Egyptians have a lower frequency of CYP1A2 * 1C , and CYP1A2 * 1E than the Japanese (0.07 vs 0.21 and 0.03 vs 0.08, respectively), while the frequencies of CYP1A2 * 1D and CYP1A2 * 1F did not differ significantly between the two groups. CYP1A2 * 1F (0.68) frequency in Egyptians was identical to that observed in Caucasians (0.68 among 236 German individuals). Conclusions The present study is the first to describe the frequencies of four known allelic variants of CYP1A2 among the Egyptian population. CYP1A2 * 1C and * 1E occurred at frequencies significantly lower than that in Japanese, while similar frequencies were observed for CYP1A2 * 1D and * 1F. The CYP1A2 * 1F frequency appeared to be identical to that of Caucasians. This does not exclude the possibility of the presence of new mutations relatively specific to the Egyptian population that have not been identified.

Interaction between CYP1A2-T2467DELT polymorphism and smoking in adenocarcinoma and squamous cell carcinoma of the lung

“…This is in line with that found in a large group of healthy Caucasian volunteers (n = 495, frequency 7.9% CYP1A2*1D [22]). The discrepancy from other results on healthy subjects −24% CYP1A2*1D frequency in a previous study of ours (n = 95) [11], 40% in an Egyptian group (n = 212) [23] and in a Japanese population (n = 159; 42%) [24]-is probably due to the low number of subjects examined in these studies. The expected genotype frequencies were not significantly different from the observed frequencies in overall lung cancer cases, SCC and adenocarcinoma separately, and controls, indicating that they were in Hardy-Weinberg equilibrium ( Table 2).…”

Genetic susceptibility to Tardive Dyskinesia in chronic schizophrenia subjects: I. Association of CYP1A2 gene polymorphism

“…15,18,29 However, it is similar to the frequency reported for African Americans. 15 Association of TD with CYP1A2*1F had earlier been reported by Basile et al 15 However, Schultze et al 20 failed to replicate this finding.…”

“…28 A very low frequency of polymorphism has been observed in CYP1A2*1C (PIC ¼ 0.1763) in this 3-MC element in our study sample. This is similar to the Egyptian population 29 but significantly different from that reported in Japanese population. 17 Consequently, absence of association of this SNP with TD in our study seems in order.…”

Frequency of –163 C > A and 63 C > G single nucleotide polymorphism of cytochrome P450 1A2 in two African populations

“…These frequencies are lower than those observed in Egyptians, Japanese and Caucasians at 68%, 65% and 71%, respectively (15,16) but are comparable to those in Ethiopians at 50% (13). While the results show an absence of the 63C)G SNP, the y163C)A occurs at a frequency of 49% and 57% in Tanzanian and Zimbabwean subjects, respectively.…”