Genotyping of Clinical Streptococcus agalactiae Strains Based on Molecular Serotype of Capsular (cps) Gene Cluster Sequences Using PCR Assay in Hamadan during 2013-2014

Mousavi, Seyed Masoud; Nassaj, Mona; Arabestani, Mohammad Reza; Nazeri, Haleh; Rahmaninan, Manijeh; Hoseinzadeh, Hasan

doi:10.18869/modares.iem.2.4.12

Cited by 1 publication

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“…While analyzing the correlation between genotypes and clinical specimens, some statistical association was observed between types Ib and III with the vaginal specimen and type II with a urine specimen in the current study. Tis is in contrast with the other study stating a statistically signifcant association between type Ia and vaginal colonization and also between type III and urine samples [1]. However, with a smaller number of samples being analyzed in each type, these associations may not be considered clinically signifcant.…”

Section: Discussioncontrasting

confidence: 76%

“…However, serotype distribution widely varies among diferent countries. Serotype III as the most common has been reported in Chinese infants [16], Japanese neonates [17], Sweden [6], Zimbabwe [18,19], Europe [20], Hamadan [1], , and Korea [21]. Similarly, serotype V as the predominant type has been reported in Egypt [22], Gambia [23], and central Africa [24].…”

Section: Discussionmentioning

confidence: 99%

“…Streptococcus agalactiae is associated with a wide variety of clinical features like urinary tract infection, wound infection, and vaginal colonization, leading to premature rupture of membranes and preterm labor, neonatal sepsis, arthritis, osteomyelitis, and septicemia in elderly individuals with other medical comorbid conditions [1]. Group B Streptococcus has nine serotypes based on capsular polysaccharides.…”

Section: Introductionmentioning

confidence: 99%

“…Te routine serotyping methods available are coagglutination, latex agglutination, counter-current immunoelectrophoresis, capillary precipitation, immunoprecipitation, enzyme immunoassay, and fuorescence microscopy [1,5,6]. However, they are labor-intensive and require high titer antisera which is not cost efective.…”

Section: Introductionmentioning

confidence: 99%

“…However, they are labor-intensive and require high titer antisera which is not cost efective. Moreover, there are certain strains that are nontypeable by these conventional methods [1]. Tereby, molecular methods to genotype this organism acts as a feasible approach since the sequences of gene clusters in all nine serotypes have been published [7].…”

Section: Introductionmentioning

confidence: 99%

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Genotype Distribution and Antibiotic Susceptibility Pattern of Clinical Isolates of Group B Streptococcus in a Tertiary Care Hospital in Puducherry, South India

Sangeetha

Devi

Subramanian

et al. 2023

Journal of Tropical Medicine

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Background. Streptococcus agalactiae apart from being a colonizer in the genital region is also associated with several other invasive infections in all age groups. With the varied distribution of serotypes across different regions of the world, universal vaccination is also unattainable. However, in India, the knowledge of group B Streptococcus (GBS) genotype distribution is deficient. Thus, this study was initiated to add data on this aspect. Methodology. A cross-sectional study was conducted using isolates of group B Streptococcus from all clinical specimens. Along with that, the clinical specimen type and the antibiotic resistance profile of the isolates were correlated with the genotypes recognized through a multiplex PCR assay. Results. Among the 86 isolates subjected to multiplex PCR for genotype identification, five genotypes were identified with genotype Ib as the predominant one (34.9%), followed by III (20.9%), II (16.3%), Ia (12.7%), and V (11.6%). Conclusion. The results demonstrated a correlation of types Ib and III with vaginal colonization and type II with urine specimens in the current study. This preliminary study exhibited the distribution of common genotypes and their antibiotic resistance profiles in various GBS isolates. However, multiple studies across the country with larger sample sizes are needed to validate these findings.

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Section: Discussioncontrasting

confidence: 76%