Genotyping of clinical Serratia marcescens isolates: a comparison of PCR-based methods

Patton, T

doi:10.1016/s0378-1097(00)00494-8

Cited by 12 publications

(17 citation statements)

References 22 publications

(24 reference statements)

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“…31 Nowadays, new molecular typing methods facilitate the recognition of epidemic strains and enhance understanding of the epidemiology of cases. [32][33][34][35][36][37][38] Nosocomial co-infections and concurrent outbreaks due to both S. marcescens and K. pneumoniae have not been reported previously. Here, we report the molecular investigation of two simultaneous clusters of cases of S. marcescens and K. pneumoniae in an NICU setting.…”

Section: Introductionmentioning

confidence: 99%

A simultaneous outbreak of Serratia marcescens and Klebsiella pneumoniae in a neonatal intensive care unit

Casolari

Pecorari

Fabio

et al. 2005

Journal of Hospital Infection

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Section: Introductionmentioning

confidence: 99%

A simultaneous outbreak of Serratia marcescens and Klebsiella pneumoniae in a neonatal intensive care unit

Casolari

Pecorari

Fabio

et al. 2005

Journal of Hospital Infection

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“…It results from the derepression of the natural class C cephalosporinase (AmpC) (23) or the expression of acquired enzymes, among which the class A extended-spectrum ␤-lactamases (ESBLs) of the TEM, SHV, and CTX-M families are the most frequently encountered (20,26,30,45). AmpC derepression and ESBL production have also been observed together in single S. marcescens strains (2,32).…”

mentioning

confidence: 80%

“…Different phenomena were documented in those works, including clonal outbreaks, the persistence of strains over prolonged periods, and recurrent outbreaks (11,26,31,32,33,41). In the case of other species of the family Enterobacteriaceae, infections caused by S. marcescens represent the tip of the iceberg, whereas colonization reflects the submerged part.…”

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confidence: 99%

Molecular Epidemiology of Serratia marcescens in Two Hospitals in Danzig, Poland, over a 5-Year Period

et al. 2004

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The history of the Serratia marcescens population in two hospitals in Danzig, Poland, over a 5-year period was analyzed in a study that combined MIC evaluation, typing by randomly amplified polymorphic DNA (RAPD) analysis and pulsed-field gel electrophoresis, and analysis of extended-spectrum ␤-lactamases (ESBLs). We analyzed 354 isolates collected from 341 patients in two teaching hospitals in Danzig, Poland, from 1996 to 2000. The antimicrobial susceptibility profiles varied greatly, and for resistance to newer ␤-lactams, probable AmpC cephalosporinase derepression and ESBL production occurred in about 23 and 19% of the isolates, respectively. RAPD typing, by which 69 types were discerned altogether, revealed a high degree of clonal diversity among the populations. However, the four most prevalent types were highly predominant, grouping approximately 71% of the isolates studied. These clones were observed in the two hospitals and were strong contributors to both outbreaks and the background of endemicity of the S. marcescens infections. Some of the strains that were not so widely spread (12 RAPD types; ϳ14% of the isolates) were responsible for several smaller outbreaks, and the remaining isolates represented unique RAPD types (53 types; ϳ15% of the isolates) and were probably sporadic introductions from other environments. ESBLs were identified in several different clones, and some of these had most likely already been introduced into the hospitals as ESBL producers, whereas the others acquired the ESBL-encoding genes from other enterobacterial strains in these environments. The CTX-M-3 enzyme, which is widely observed in Poland, was the most common ESBL type among the S. marcescens isolates, followed by TEM-47 and SHV-5. The complex epidemiology of ESBLs, especially in 1999 and 2000, must have arisen from the introduction of ESBL producers from other centers, their clonal dissemination, and the constant penetration of the S. marcescens populations with plasmids with ESBL genes. Multiple S. marcescens isolates were obtained from 11 patients, who probably represented both patients with recolonizations and reinfections and patients with recurrences of infections with the evolution of the strain's susceptibility.Serratia marcescens has been reported to be a significant agent of hospital infections since the 1950s. Because it is an opportunistic pathogen, it mainly affects patients in neonatal and surgical units and patients in intensive care units (ICUs). It is also responsible for various types of infections, including pneumonia, sepsis, meningitis, and fasciitis (1, 45). Treatment of these infections is often very difficult, which to a big extent is due to the widespread natural and acquired resistance of the organism to antimicrobials. S. marcescens strains with combined resistance to expanded-spectrum ␤-lactams, aminoglycosides, fluoroquinolones, and/or co-trimoxazole have been identified in numerous hospitals worldwide (1,20,23,35). As in other enterobacteria, resistance to newer ␤-lactams is usually ␤-lact...

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“…Serratia marcescens, formerly known as Chromobacterium prodigiosin [6] is a gram-negative facultative anaerobe [7] that has emerged in recent times as a nosocomial pathogen. It functions as an opportunistic organism in immunocompromised patients because of its invasive ability to adhere to hospital instrumentation such as catheters, endoscopes and intravenous tubing [8].…”

Section: Introductionmentioning

confidence: 99%

Antibiotic Susceptibility and Heavy Metal Tolerance Pattern of Serratia Marcescens Isolated From Soil and Water

Nageswaran¹,

Ramteke²,

Verma³

et al. 2012

J Bioremed Biodegrad

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The antibiotic and metal tolerance patterns of Serratia marcescens strains isolated from soil and water around the Sangam region of Allahabad were obtained. Using the standard minimum inhibitory concentration (MIC) for each antibiotic respectively, the Kirby-Bauer disc-diffusion method was used to obtain antibiotic resistance patterns of the Serratia strains and the MIC of the metals -chromium, cadmium, cobalt, copper, lead and nickel for each of the strains were also obtained. Plasmid curing was carried out for specific antibiotic and metal resistances to ascertain plasmid-borne transfer of resistance genes. Results obtained showed that Multi-drug resistant (MDR) strains of Serratia were resistant to certain metals as well suggesting specific metal-antibiotic resistant gene patterns in the different strains. Journal of Bioremediation & Biodegradation

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Genotyping of clinical Serratia marcescens isolates: a comparison of PCR-based methods

Cited by 12 publications

References 22 publications

A simultaneous outbreak of Serratia marcescens and Klebsiella pneumoniae in a neonatal intensive care unit

A simultaneous outbreak of Serratia marcescens and Klebsiella pneumoniae in a neonatal intensive care unit

Molecular Epidemiology of Serratia marcescens in Two Hospitals in Danzig, Poland, over a 5-Year Period

Antibiotic Susceptibility and Heavy Metal Tolerance Pattern of Serratia Marcescens Isolated From Soil and Water

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