Genotyping of clinical and environmental Aspergillus flavus isolates from Iran using microsatellites

Abstract

Aspergillus flavus is the second most important Aspergillus species causing human infections in tropical countries. Despite an increasing number of infections of A. flavus in Iran, the molecular epidemiology of clinical and environmental strains has not been well studied. We used a panel of nine microsatellite markers to analyse the genetic relatedness of A. flavus. Microsatellite typing of 143 (n = 119 clinical and n = 24 environmental) isolates demonstrated 118 different genotypes. A possible outbreak at a pulmonary ward was discovered. The discriminatory power for the individual markers ranged from 0.4812 to 0.9457 and the panel of all nine markers combined yielded a diversity index of 0.9948. This high-resolution typing method assists in better understanding of the molecular epidemiology of A. flavus.

Molecular Characterization and Antifungal Susceptibility of Clinical Fusarium Species From Brazil

“…Final identification was done using molecular methods. DNA extraction was performed as described by Khodavaisy et al (2016). Conidia were suspended in 400 μL bacterial lysis buffer (Roche Diagnostics, Almere, Netherlands) followed by mechanical lysis in a MagNA Lyser (Roche Diagnostics) for 30 s at 4,500 × g .…”

Proven Aspergillus flavus pulmonary aspergillosis in a COVID‐19 patient: A case report and review of the literature

“…In our case, despite voriconazole therapy, the patient died due to concomitant involvement with coronavirus and failure to antifungal therapy. In A fumigatus, triazole resistance should be considered in azole‐treatment failure, but in our case, A flavus was recovered, which is the predominant Aspergillus species causing aspergillosis in Iran 17,33,34 …”

“…In A fumigatus, triazole resistance should be considered in azole-treatment failure, but in our case, A flavus was recovered, which is the predominant Aspergillus species causing aspergillosis in Iran. 17,33,34 In conclusion, recovery of Aspergillus species in a critically-ill COVID-19 patient should not be ignored, but requires a diagnostic workup despite suboptimal performance of relevant biomarkers.…”

Use of cell surface protein typing for genotyping of azole‐resistant and ‐susceptible Aspergillus fumigatus isolates in Iran

“…Molecular typing is an indispensable technique for outbreak analysis, environmental monitoring and the study of local and global epidemiology . Several genotype‐based methods such as random amplified polymorphic DNA (RAPD), restriction fragment length polymorphism (RFLP) and amplified fragment length polymorphism (AFLP) have been used to distinguish closely related organisms at the species to strain level . Methods based on the detection of single nucleotide polymorphisms (SNPs), such as multi‐locus sequencing typing (MLST), are used extensively because the high levels of resolution, the ability to characterise a large number of isolates rapidly and absence of any subjective interpretation of banding patterns .…”