Genotyping of Acanthamoeba isolates from clinical and environmental specimens in Iran

Niyyati, Maryam; Lorenzo‐Morales, Jacob; Rezaie, Sasan; Rahimi, Firoozeh; Mohebali, Mehdi; Maghsood, Amir Hossein; Motevalli-Haghi, Afsaneh; Martín-Navarro, Carmen M.; Farnia, Shohreh; Valladares, Basilio; Rezaeian, Mostafa

doi:10.1016/j.exppara.2008.11.003

Cited by 88 publications

(79 citation statements)

References 23 publications

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“…These results are in accordance with findings of various authors, that the genotype T4 is the most prevalent genotype in the environment and most widely spread worldwide (Stothard et al 1998;Schuster & Visvesvara 2004). Acanthamoeba of this genotype were isolated, among other sources, from areas such as beaches in south Florida (Booton et al 2004), environmental samples in Bulgaria (Tsvetkova et al 2004), from the Philippines (Rivera & Adao 2008) as well as from Iran (Niyyati et al 2009). …”

Section: Discussionmentioning

confidence: 99%

Morphological, physiological, molecular and phylogenetic characterization of new environmental isolates of Acanthamoeba spp. from the region of Bratislava, Slovakia

Nagyová

Nagy²,

Janeček

et al. 2010

Biologia

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Protozoa of the genus Acanthamoeba are organisms that can be generally found in the environment. The focus of this study is the detection of the presence of Acanthamoeba in different water sources and samples taken from airconditioning units. The identification of Acanthamoeba isolates was based on the morphology of cysts and trophozoites as well as PCR amplification with a genus specific primer pair JDP1 and JDP2. Growth characteristics and temperature tolerance were monitored. The pathogenic potential was tested in vitro on Vero cell cultures. Genotype identification was based on the sequencing of the GTSA.B1 PCR amplimer of 18S ribosomal DNA. The data obtained revealed that the isolates belong to T3 and T4 genotypes. One T3 and one T4 isolate contain a group I intron. The 933 base pair intron found in a genotype T4 isolate is considerably larger compared to formerly described introns of Acanthamoeba griffini (genotype T3) and A. lenticulata (genotype T5). This is the first report detailing the environmental distribution of the Acanthamoeba genotypes in the region of Bratislava, Slovakia.

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Section: Discussionmentioning

confidence: 99%

Morphological, physiological, molecular and phylogenetic characterization of new environmental isolates of Acanthamoeba spp. from the region of Bratislava, Slovakia

Nagyová

Nagy²,

Janeček

et al. 2010

Biologia

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“…Sequences were aligned using the MEGA5 software program (Tamura et al, 2011). Genotype identification was based on sequence analysis of the DF3 region as previously described by comparison with the available Acanthamoeba DNA sequences in the GenBank database (Booton et al, 2005;Niyyati et al, 2009). Acanthamoeba castellanii Neff ATCC 30010 DNA was used as a positive control in the PCRs.…”

Section: Methodsmentioning

confidence: 99%

“…18S rDNA gene amplifications for Acanthamoeba (DF3 region) were performed as previously described from both DNA extracts from the strips and positive cultures (Booton et al, 2005;Niyyati et al, 2009). The resulting PCR products were purified using the Qiaquick PCR purification kit (Qiagen) and sequenced using a MEGABACE 1000 automatic sequencer (Healthcare Biosciences) in the University of La Laguna sequencing service (Servicio de Secuenciació n SEGAI, Fig.…”

Section: Methodsmentioning

confidence: 99%

Detection of Acanthamoeba on the ocular surface in a Spanish population using the Schirmer strip test: pathogenic potential, molecular classification and evaluation of the sensitivity to chlorhexidine and voriconazole of the isolated Acanthamoeba strains

Rocha-Cabrera

Reyes-Batllé

Martín-Navarro

et al. 2015

Journal of Medical Microbiology

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“…The ability of these protozoans to survive and proliferate in nature, especially water, shows that they can be potentially pathogenic for humans and animals (7). According to the wide dispersion of protozoan, it is expected that many people are exposed to protozoan (20)(21)(22)(23)(24)(25)(26); therefore, it seems that 80% of the people have antibodies against protozoa (27).…”

Section: Discussionmentioning

confidence: 99%

Soil Contamination With Free-Living Amoeba in North of Iran

et al. 2016

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Background: Free-living amoebas are very abundant in nature, especially in water and soil. Some species of amoebas cause serious and sometimes fatal infections such a keratitis in people with normal and suppressed immune systems. Based on the prevalence of amoebic keratitis in Iran, it is important to consider the free-living amoeba. Objectives: The current study aimed to determine the presence of Acanthamoeba and Hartmannella spp. in soil samples of Sari, North of Iran. Patients and Methods: A total of 96 soil samples from three areas of Sari were collected to be examined for soil contamination with Acanthamoeba and Hartmannella spp. Soil samples were investigated for the presence of these parasites by Sheather's flotation technique and Gimsa staining method. The identification of Acanthamoeba and Hartmannella spp. at the genus level in this study was based on distinctive features of double walled cysts. Results: Of the 96 studied samples, from different environmental locations in Sari, 38 (39.6%) Acanthamoeba and 5 (5.2%) Hartmannella species were identified. Conclusions:The results of the present study revealed that soil resources of these areas were contaminated with opportunistic amoebas such as Acanthamoeba and Hartmannella spp. and this may lead to severe diseases in high-risk people, such as immunocompromised patients.

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Genotyping of Acanthamoeba isolates from clinical and environmental specimens in Iran

Cited by 88 publications

References 23 publications

Morphological, physiological, molecular and phylogenetic characterization of new environmental isolates of Acanthamoeba spp. from the region of Bratislava, Slovakia

Morphological, physiological, molecular and phylogenetic characterization of new environmental isolates of Acanthamoeba spp. from the region of Bratislava, Slovakia

Detection of Acanthamoeba on the ocular surface in a Spanish population using the Schirmer strip test: pathogenic potential, molecular classification and evaluation of the sensitivity to chlorhexidine and voriconazole of the isolated Acanthamoeba strains

Soil Contamination With Free-Living Amoeba in North of Iran

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