Genotyping of 73 UM‐SCC head and neck squamous cell carcinoma cell lines

Abstract

Background We established multiple UM-SCC (University of Michigan Squamous Cell Carcinoma) cell lines. With time, these have been distributed to other labs all over the world. Recent scientific discussions have noted the need to confirm the origin and identity of cell lines in grant proposals and journal articles. We genotyped the UM-SCC cell lines in our collection to confirm their unique identity. Design Early passage UM-SCC cell lines were genotyped and photographed. Results Thus far, 73 unique head and neck UM-SCC cell lines (from 65 donors including 21 lines from 17 females) were genotyped. In 7 cases separate cell lines were established from the same donor. Conclusions These results will be posted on the U of M Head and Neck SPORE Tissue Core website for other investigators to confirm that the UM-SCC cells used in their laboratories have the correct features. Publications using UM-SCC cell lines should confirm the genotype.

Neutrophils Increase Oral Squamous Cell Carcinoma Invasion through an Invadopodia-Dependent Pathway

“…UMSCC47 has a wild-type TP53 gene and is positive for HPV16 (20). UMSCC1 is derived from a man with a nonmetastatic floor of mouth squamous cell carcinoma (21). Both UMSCC1 and UMSCC47 were recently shown to represent a unique genetic background and to be free of contamination (20).…”

Small Molecule Inhibition of MDM2–p53 Interaction Augments Radiation Response in Human Tumors

“…Radiation survival curves were plotted after normalization for the cytotoxicity induced by control or drug alone. Cell viability was examined either by crystal violet staining or propidium iodide (PI) dye exclusion flow cytometric assay as described previously (12). Following staining with PI (30 mg/mL) for 30 minutes, dead cells permeable to PI were quantified by analyzing the geometric mean fluorescence intensity (GMFI) of PI in 10,000 cells.…”

Model examinatio�n of chemoprevention with retinoids in squamous cell carcinomas of the head and neck region and suitable biomarkers for chemoprevention

“…On the same model cells we compared the proliferation before and after retinoid therapy as well as the expression of several retinoid receptors, from Ki-67 and p53. We used the two HNSCC cell lines UM-SCC-14C and UM-SCC-35 (119,120) and controlled with the spontaneously immortalized aneuploid human keratinocyte cell line HaCaT (121). The doubling time of UM-SCC-35 is between 38 (122) and 52 h (123), of UM-SCC-14 C it is ~26 h (119,120,123).…”

“…Schroeder and Zouboulis (128) were also able to demonstrate dose-dependent inhibition of growth in HaCaT-cells, more clearly by 13-cRA than by ATRA. (119,120) isolated the tumor cell lines from fresh head and neck cancer tissues: UM-SCC-35 for a primary tonsil carcinoma (T4N1M0, moderately well differentiated) and UM-SCC-14C for a skin metastasis of a floor and mouth carcinoma (poorly differentiated) after chemotherapy. The spontaneously immortalized aneuploid human keratinocyte cell line HaCaT was established by Boukamp et al (121) from the tumor-free skin area surrounding a melanoma.…”

“…Carcinoma tissue and control tissues were examined immunohistochemically. Cell proliferation before and after the effects of ATRA was also performed on the three cell lines UM-SCC-35, UM-SCC-14C (119,120) and HaCaT (121) in 96-well plates and the telomerase activity in all three cell lines before and after ATRA treatment was examined.…”