2019
DOI: 10.1016/j.vetmic.2019.03.016
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Genotyping Mycoplasma gallisepticum by multilocus sequence typing

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Cited by 21 publications
(18 citation statements)
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“…MG can spread within members of one order, which could be evidence of a single ancestor [23]. A recent study which summarized the phylogeny of MG showed that one strain of MG could be isolated from three different poultry species [89]. It has now been hypothesized that the adaptation of MG to a new host is not so easy and needs certain favorable factors helping it PLOS ONE [90,91].…”
Section: Discussionmentioning
confidence: 99%
“…MG can spread within members of one order, which could be evidence of a single ancestor [23]. A recent study which summarized the phylogeny of MG showed that one strain of MG could be isolated from three different poultry species [89]. It has now been hypothesized that the adaptation of MG to a new host is not so easy and needs certain favorable factors helping it PLOS ONE [90,91].…”
Section: Discussionmentioning
confidence: 99%
“…In this study, 40 isolates of Mycoplasma gallisepticum were genetically characterized. These isolates were collected from chickens (21), turkeys (14), geese (2), quail (1), and guinea fowls (2), during the years 2010-2019 (Table 1). More than 90% of these samples were of industrial poultry origin, the remaining were backyard or private producer avian samples from different farming areas, mainly from the Italian northern regions (Piemonte, Lombardia, Veneto, Emilia-Romagna).…”
Section: Sample Selection and Mycoplasma Isolationmentioning
confidence: 99%
“…Six selected housekeeping genes (atpG, dnaA, fusA, rpoB, ruvB, uvrA) were amplified in separate tubes in a 20 µL total volume using the primers listed in Table S1. Each reaction mix contained 10 µL of Sybr Fast Universal Master Mix (2X) (Merck Life Science, Milan, Italy), the specific primer pair at the concentration reported in Beko et al [14] and 1.5 µL of template DNA. The thermal profile consisted of an initial denaturation/enzyme activation for 5 min at 95 • C followed by 35 cycles comprising a denaturation step at 95 • C for 1 min, primer annealing at 56 • C for 30 s and extension at 72 • C for 1 min.…”
Section: Mg Mlstmentioning
confidence: 99%
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