GenotypingCagA,VacAsubtype,IceA1, andBabAofHelicobacter pyloriisolates from Korean patients, and their association with gastroduodenal diseases

Abstract

The genetic status of cagA, vacA subtype, iceA1, and babA, and the relationship to gastroduodenal diseases were assessed in Helicobacter pylori isolates in Korea. Seventy-six strains of H. pylori were isolated from the antrum and the corpus of 41 adult patients (22 with peptic ulcer and 19 with gastritis). The cagA, iceA1, and babA genes were assessed by polymerase chain reaction and the vacA subtypes were determined by reverse hybridization-line probe assay. The positive rates of 349-bp cagA, 208-bp cagA, iceA1, and babA genes were 97.4%, 96.1%, 84.2%, and 36.1%, respectively. The vacA s1a, s1b, s1c, and s2 variants were detected in 11.8%, 3.9%, 80.4%, and 1.3%, respectively. m1 (78.9%) is more prevalent than m2 (5.3%). The most common vacA genotype was s1c/m1 (61.9%), and 14 isolates (18.4%) contained mixed vacA genotypes from a single biopsy specimen. Twenty-one (60%) of 35 patients were infected with more than two strains of different cagA, iceA1, babA, and vacA genotypes. None of cagA, iceA1, babA, and vacA s1/m1 were associated with peptic ulcer. In conclusion, most H. pylori isolates in Korea carry cagA, iceA1, and vacA s1c/m1 genes, and reside with multiple strains. These genes do not correlate with the peptic ulcer in the Korean patients.

Prevalence of genotypes in Helicobacter pylori isolates from patients in eastern Turkey and the association of these genotypes with clinical outcome

“…In an attempt to detect association between the cagE gene and PU, In contrast to the results reported in China, Japan, Korea and Netherlands where the iceA1 gene was predominant (16,20,31), the iceA2 gene was detected to be predominant genotype in this study. This result is in agreement with previous reports that the iceA2 gene was found to be prevalent in Brazilian, European and American patients (1,20,22).…”

“…Our results were similar to a previous study (1) (12,19,29) while it was 80-100% in Asian countries (16). We found a higher prevalence (62.1%) of babA2 than the result (53.8%) reported in a previous study (10) in Turkey.…”

Correlation of Helicobacter pylori Genotypes with Gastric Histopathology in the Central Region of a South-European Country

“…One of the most important virulence factors of H. pylori is cagA that is present in about 60-70 % of the Western strains and in more than 90 % of the East Asian ones [7, 28,29]. In our study cagA genotype, albeit determined by real-time PCR using two different sets of primers, was identified in only 31.8 % of isolates.…”

“…The H. pylori vacA s1m1 genotype is the most common in American, European and East Asian countries [24,29,[41][42][43]. The vacA s2m2 genotype is apparently less common and another study in Portugal, performed in patients submitted to a screening program, revealed a prevalence of 29.7 % for such allele combination (42.9 % if multiple vacA genotypes were excluded).…”

Investigation of Association between oipA and iceA1/iceA2 Genotypes of Helicobacter pylori and Gastric Cancer in Iran

“…In addition, cagA and the vacuolating cytotoxin (vacA) genotypes are proposed to serve as predictors for progression of gastric lesions (Gonzalez et al, 2011). Moreover, presence of other genes of H pylori such as iceA (induced by contact with epithelium), oipA (outer inflammatory protein), and babA (blood group antigenbinding adhesin), has been reported to be associated with development of gastroentric disorders (Kim et al, 2001;Markovska et al, 2011). Most of these genes encode the proteins that help H pylori interact with host or change host cellular homeostasis, which ultimately lead to abnormalities like neoplastic tissues (Ilver et al, 1998;Naumann, 2005).…”