Genotyping and virulence factors assessment of bovine mastitis Escherichia coli

Blum, Shlomo E.; Leitner, Gabriel

doi:10.1016/j.vetmic.2012.12.037

Cited by 63 publications

(98 citation statements)

References 33 publications

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“…Also in mastitis isolates, group B1 was not observed but group A 1 was found to be the most prevalent group by triplex PCR which supported the hypothesis of mastitis isolates mostly comprising commensal strains of E. coli. Our results were also found in accordance with the previous studies on bovine mastitis in which commensal groups A and/or B1 were found to be the most prevalent phylo-groups (Dogan et al 2012;Blum & Leitner 2013). According to the new quadruplex PCR method, group B1 and C were found to be the predominant groups with 45% and 37% in mastitis isolates, respectively.…”

Section: Resultssupporting

confidence: 91%

Phylo-typing of clinicalEscherichia coliisolates originating from bovine mastitis and canine pyometra and urinary tract infection by means of quadruplex PCR

Müştak

Günaydin²,

Kaya

et al. 2015

Veterinary Quarterly

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(2015) Phylo-typing of clinical Escherichiacoli isolates originating from bovine mastitis and canine pyometra and urinary tract infection by means of quadruplex PCR, Veterinary Quarterly, 35:4, 194-199, DOI: 10.1080/01652176.2015 Background: Escherichia coli is one of the major causative agents of bovine mastitis worldwide, and is typically associated with acute, clinical mastitis. Besides this, E. coli strains which belong to the extra-intestinal pathogenic group are also the major cause of urinary tract infections and pyometra in dogs. Objectives: In this study, it was aimed to investigate phylo-groups/subgroups in 155 E. coli isolates obtained from acute bovine mastitis, 43 from urinary tract infections of dogs and 20 from canine pyometra by a formerly described triplex PCR and recently described new quadruplex polymerase chain reaction (PCR) method. Results: Group A 1 (n D 118; 76%) and B1 (n D 71; 46%) were found to be the most prevalent groups by triplex and quadruplex PCR assays in mastitis isolates, respectively. Phylo-typing of 43 urinary tract isolates also revealed that most of the isolates belonged to A 1 (n D 23; 54%) by triplex and B2 (n D 36; 84%) by quadruplex PCR assays. The isolates assigned as group A 1 (n D 17; 85%) by triplex PCR could not be classified by quadruplex PCR in pyometra isolates. Conclusions:The results support the hypothesis that E. coli strains isolated from bovine mastitis cases are environmental. Also, groups C, E and F were identified as new phylo-groups for the first time in acute bovine mastitis cases. The comparison of triplex PCR with quadruplex PCR results revealed that most of the groups assigned in triplex PCR were altered by quadruplex PCR assay.

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Section: Resultssupporting

confidence: 91%

Phylo-typing of clinicalEscherichia coliisolates originating from bovine mastitis and canine pyometra and urinary tract infection by means of quadruplex PCR

Müştak

Günaydin²,

Kaya

et al. 2015

Veterinary Quarterly

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“…Phylogroup E was designated as comprising the unassigned strains of O157:H7 which is enterohemorrhagic classified under intestinal pathogenic E. coli [11] . According to the results of this study, most of E. coli isolates from bovine acute mastitis were determined as commensal and intestinal origin in compatible with the previous studies [17,26,30] . Newly designated phylogroup F has been termed as a sister group to phylogroup B2 [30] .…”

Section: Discussionsupporting

confidence: 88%

“…The least two phylogroups determined from acute bovine mastitis were found to be D and F. Most of the commensal and intestinal strains have been reported as group A and B1, extra-intestinal E. coli strains belonged to group B2 and D in other studies [6,7] . In the previous studies, bovine mastitis isolates have been generally belonged to A and B1 groups [8,9,[26][27][28] . However, phylogroup C was defined to be closely related to, but distinct from B1 [29,30] .…”

Section: Discussionmentioning

confidence: 99%

Akut Sığır Mastitislerinden İzole Edilen Escherichia coli Suslarının Filogruplandırılması ve Virulens Faktör Değerlendirmesi

Günaydin¹,

Müştak²,

Önat³

et al. 2017

Kafkas Univ Vet Fak Derg

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The objective of this study was to determine the phylogenetic distribution of commonly studied virulence factors of 155 E. coli isolated from acute bovine mastitis. In the study, A, B1, B2, C, D, E, F phylogroups, and Clade I were defined. B1 and C were found to be the most prevalent phylogroups covering commensal strains with a ratio of 45.2% and 37.4%, respectively. The other phylogroups determined in the study were F, D, A, and E with a range of 0.6%-8.9%. Commonly known virulence genes; clpg, F17, afa, iucC, iucD, cnf1, cnf2, kps, and traT were selected to determine the virulence factors. The only virulence factor gene existing within the strains was traT (66.4%). However, any relation between phylogroups and virulence factors was not determined. Phylogroups C, E and F were found to be new phylogroups for the first time in acute bovine mastitis cases. Keywords: Escherichia coli, Bovine mastitis, Phylogroup, Virulence factor Akut Sığır Mastitislerinden İzole Edilen Escherichia coli Suslarının Filogruplandırılması ve Virulens Faktör Değerlendirmesi ÖzetÇalışmanın amacı akut sığır mastitinden izole edilen 155 E. coli izolatında, yaygın olarak çalışılan virulens faktörlerinin filogenetik dağılımını belirlemekti. İzolatların filogruplandırması yapıldığında, A, B1, B2, C, D, E, F grupları ve Sınıf I tespit edildi. Kommensal suşları oluşturan, B1 ve C sırasıyla, %45.2 ve %37.4 oranla en yaygın filogruplar olarak bulundu. Bu çalışmada, diğer filogruplar, %0.6-%8.9 aralığındaki oranda F, D, A, and E olarak tespit edildi. Virulens faktörlerini belirlemek için en çok bilinen virulens genleri; clpg, F17, afa, iucC, iucD, cnf1, cnf2, kps ve traT seçildi. traT, suşların büyük çoğunluğunda (%66.4) tespit edilen virulens genidir. Filogruplar ve virulens faktörleri arasında herhangi bir ilişki belirlenmedi. Akut sığır mastitis olgularında, C, E. F filogrupları ilk kez tespit edildi.

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“…Enrofloxacin the most effective drugs against S. aureus [16] . The highest resistance rate of S. aureus against Penicillin [66; 47.6; 47.6 and 56.5%] was reported by [2,25,26,27] , respectively. An unusual high prevalence of MRSA in Belgian cases of subclinical and clinical S. aureus mastitis in cows [28] .…”

Section: Discussionmentioning

confidence: 99%

A contribution on Coliforms causing mastitis in cows with reference to serotypes and virulence factors of E. coli isolates

Sayed¹

2016

AJMI

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How to cite this article:Eighteen E. coli isolates were serotyped to nine different serogroups; O111:H4 (3), O127:H6 (3), O26 (2), O126 (2), O119:H6(1), O114:H21 (1), O55:H7 (1), O44:H18 (1), O124 (1) and (3) untyped. Virulence tests were performed on the 18 isolated E.coli, it was found that 15 isolates (83.3%) were serum resistant, 13 isolates (72.2%) had Congo Red binding activity, 6 isolates (33.3%) were invasive and one isolate (5.6%) had haemolytic activity. PCR was applied to detect the presence of Shiga like toxin producing E. coli (stx1 and stx2 genes) on the nine different strains (one strain for each serogroup), where stx1 and stx2were found in 8 (88.9%) and 4 (44.4%) of the nine examined strains, respectively. While stx1 and stx2 genes were found together in 3 strains (33.3%). Conclusions: E. coli isolates usually posses one or more virulence factors that may help in establishment at the infection site and subsequently causing clinical bovine mastitis. Keywords:Coliforms, E. coli, Serotypes, Virulence Factors, Stx1, Stx 2. Sayed, S. M.Sayed, S.M., Egypt-Animal Health Research Institute (Assiut Regional Lab., Bacteriology Dept.)ABSTRACT AJMI: http://escipub.com/american-journal-of-microbiology-and-immunology/ 0010

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Genotyping and virulence factors assessment of bovine mastitis Escherichia coli

Cited by 63 publications

References 33 publications

Phylo-typing of clinicalEscherichia coliisolates originating from bovine mastitis and canine pyometra and urinary tract infection by means of quadruplex PCR

Phylo-typing of clinicalEscherichia coliisolates originating from bovine mastitis and canine pyometra and urinary tract infection by means of quadruplex PCR

Akut Sığır Mastitislerinden İzole Edilen Escherichia coli Suslarının Filogruplandırılması ve Virulens Faktör Değerlendirmesi

A contribution on Coliforms causing mastitis in cows with reference to serotypes and virulence factors of E. coli isolates

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