Genotyping and Genetic Diversity Analysis of 47 Flowering Cherry (<I>Cerasus</i>) Germplasms

Abstract

In order to provide a theoretical basis for conservation and utilization of excellent flowering cherry germplasms, genetic diversity and genotype were analyzed based on single nucleotide polymorphism (SNP) molecular markers. A total of 47 germplasms were sequenced using restriction site-associated DNA sequencing (RAD-Seq) method, and highly consistent SNP sites have been identified for genetic diversity analysis and genotyping. The RAxML program was used for phylogenetic tree construction, based on the maximum likelihood (ML) method. The ADMIXTURE software was used to analyze genetic structure. The GCTA software was used for principal component analysis. Genetic diversity parameters, includes nucleotide diversity (π value), expected heterozygosity (exp He) and genetic differentiation index (FST), were carried out using the PopGenome and Arlequin 3.0 software. A total of 79 667 highly consistent SNPs were obtained, after genotype integrity filtering and closely linked sites screening. According to the phylogenetic, principal components and population structure analysis results, the 47 germplasms could be divided into eight genetic clusters. The π and exp He value of each cluster was as follows:

Evolution of Rosaceae Plastomes Highlights Unique Cerasus Diversification and Independent Origins of Fruiting Cherry

“…Hybridization events among Cerasus taxa have been widely reported (Ohta et al, 2007;Shi et al, 2013;Kato et al, 2014;Baek et al, 2018). Especially, a recent analysis based on whole-genome re-sequencing data indicates that four potential inter-specific hybridization events have occurred among Cerasus taxa (Yan et al, 2020). Therefore, we inferred that the discordance and lack of phylogeographic signals might have resulted from potential multiple hybridization events within Cerasus.…”

Performance Evaluation of Exotic and Local Landraces of Tomatoes for the Mid-Hill Conditions of Nepal

“…In contrary to their report, the yield of AVTO lines in our study was low which was due to heavy rainfall and disease problem. Chapagain et al (2020a) found that the highest positive effect of marketable fruit per plant (r=0.99) on fruit yield per plant. Correlation analysis revealed that plant height, fruit weight and diameter were positively associated with fruit yield (Reddy et al, 2013;Chapagain et al, 2020a).…”

“…Chapagain et al (2020a) found that the highest positive effect of marketable fruit per plant (r=0.99) on fruit yield per plant. Correlation analysis revealed that plant height, fruit weight and diameter were positively associated with fruit yield (Reddy et al, 2013;Chapagain et al, 2020a). Chapagain et al (2020a) reported that fruit diameter showed positive and significant correlation with average fruit weight (0.983).…”

“…This suggests that yield is not only a genetic factor, it is also governed by the environment (Bhurtyal et al, 2007;Devkota et al, 2018). Chapagain et al (2020a) studied in tomatoes for fruit yield and yield contributing traits and were grouped into one cluster having taller plants with higher marketable fruits per plant, fruit set percentage, flowers per inflorescence, marketable fruits and fruit yield per plant. Tomato genotypes with semi-determinate to indeterminate growth habit with medium sized fruits performed better in yield and quality attributes (Chapagain et al, 2020b).…”

“…Correlation analysis revealed that plant height, fruit weight and diameter were positively associated with fruit yield (Reddy et al, 2013;Chapagain et al, 2020a). Chapagain et al (2020a) reported that fruit diameter showed positive and significant correlation with average fruit weight (0.983). It was also found that there was strong positive correlation between fruit number per inflorescence and marketable fruit per plant.…”