Genotyping and drug susceptibility testing of mycobacterial isolates from population-based tuberculosis prevalence survey in Ghana

Addo, Kennedy Kwasi; Addo, Samuel; Mensah, Gloria Ivy; Mosi, Lydia; Bonsu, Frank

doi:10.1186/s12879-017-2853-3

Cited by 25 publications

(36 citation statements)

References 16 publications

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“…15,16 The occurrence of 531 mutations (11.7%) in this study was comparable to 9.4% found in the previous study. 10 Our finding and those of Burkina Faso neighboring countries 11,[12][13][14] contrast enormously with the results of numerous studies [15][16][17][18][19][20][21][22][23][24][25][26][27][28][29] which reported higher rates of mutations at 531. High rates of 531 mutations were particularly found in Kyrgyzstan, Ethiopia, Brasil, South West Ethiopia, Georgia, Taiwan and Samara in Russia.…”

Section: Discussioncontrasting

confidence: 99%

“…For instance, the frequency of rpoB531 mutations is much lower in RMPresistant strains in China where the frequency of the Beijing genotype strains is high. 31 The occurrence of mutation at 526 reported in the current study is higher than rate found in Burkina Faso neighbouring Countries, 11,[12][13][14] lower than the value reported in Nigeria 6 and similar to findings reported from India and Kyrgyzstan. 2,21 All this indicates that mutations at codons 531, 526 and 516 are common, with a varied occurrence across the geographical areas.…”

Section: Discussionsupporting

confidence: 78%

“…In this study, occurrence of gene mutation attributed to low level drug resistance mainly caused by the mutations in the promoter region of inhA gene was lower than the mutation frequent C15T observed in Ivory Coast. 13 Occurrence of katG315 mutations was lower than those obtained in Ghana and in Southwest Ethiopia, 11,25 similar to an Ivorian study 12 , and higher than a report from Nigerian. 6 The high occurrence (90-95%) of katG315 mutations among M. tuberculosis strains in Russia was attributed to the high frequency of Beijing genotype strains.…”

Section: Discussionsupporting

confidence: 69%

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Occurrence of mutations associated with rifampicin and isoniazid resistant in Mycobacterium tuberculosis isolates from patients in Burkina Faso

Diandé¹,

Ogbomon²,

Gueye³

et al. 2019

IJMBOA

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Genetic mutations are responsible for the high rate of resistance observed in the treatment of tuberculosis. This study aimed at determining the occurrence of mutations associated with rifampicin (RIF) and isoniazid (INH) resistance of Mycobacterium tuberculosis complex (MTBC) isolates. MTBC strains isolated by culture from 110 TB patients diagnosed with resistant to rifampicin (RR-TB) by Xpert MTB/RIF were studied. The isolates were obtained from the National Tuberculosis Reference Laboratory in Ouagadougou. They were identified culturally using Antigenic method (SD Bioline TB Ag MPT64). Polymerase Chain Reaction, PCR (DRplus) was used to detect the occurrence of mutations in the genes associated with resistance katG and inhA promoter for INH, and rpoB for RIF. Out of 103 isolates with RIF resistant, mutations were detected in 87(84.5%) of gene rpoB while no mutation was found in 16(15.5%) of the gene of the isolates even though the wild probes had disappeared. Single mutations were found in the codons D516V (41.7%) and H526Y (17.5%) while combined mutations (single and double) were mostly detected in the codons D516 (51.5%), H526Y (20.4%), S531L (11.7%) and H526D (10.7%) respectively. Single mutations responsible for high-level isoniazid resistance, katG were observed in the codon S315T1 while the combined inhA and katG were detected in the codon C8T and S315T, 16 (14.5%) respectively. The highest mutation occurrence was observed with rpoB516, rpoB526 for RIF and katG315 for INH associated with resistance of MTBC isolates. There is a need to improve molecular assay kit diagnosis to curb the geographic specificity of the target genes needed to detect more possible mutations.

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Section: Discussioncontrasting

confidence: 99%

Section: Discussionsupporting

confidence: 78%

Section: Discussionsupporting

confidence: 69%

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Occurrence of mutations associated with rifampicin and isoniazid resistant in Mycobacterium tuberculosis isolates from patients in Burkina Faso

Diandé¹,

Ogbomon²,

Gueye³

et al. 2019

IJMBOA

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“…A report from central Ethiopia also in line with our nding that no gain of any mutant band was identi ed for the non-hybridized WT8 probe [21]. Similar ndings were also reported for the absence of MUT band from other countries [36,37]. Despite such reports, there were research ndings where gain of MUT probes was identi ed in a part of the rpoB gene [17].…”

Section: Frequency Of Gene Mutations Associated With Rifampicin and Isupporting

confidence: 87%

Phenotypic and genotypic drug sensitivity profiles of Mycobacterium tuberculosis infection and associated factors in northeastern Ethiopia

Gashaw

Erko

Mekonnen

et al. 2020

Preprint

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Background Tuberculosis is a devastating and a deadly disease despite the novel advances in its diagnostic tools and drug therapy. Drug resistant Mycobacterium contributes a great share to tuberculosis mortality. Status of drug resistance and patients’ awareness toward the disease is unknown in northeastern Ethiopia. Thus, the aim of this study was to determine the phenotypic and genotypic drug sensitivity patterns and associated factors in Oromia Special Zone and Dessie Town, northeastern Ethiopia. Methods In a cross-sectional study, 384 smear positive tuberculosis cases were recruited and Löwenstein-Jensen culture was done. The performance of GenoTypic MTBDRplus assay using the conventional BACTEC MGIT 960 as a "gold standard" was determined. Drug resistant strains were identified using spoligotyping. Pearson Chi-square test was used to determine the association of drug sensitivity test and tuberculosis type, lineages, dominant strains and clustering of the isolates. For the assessment of patients’ awareness towards the disease questionnaires were used. Results A fair agreement was found between MTBDRplus assay and the conventional MGIT test in detecting the Mycobacterium tuberculosis with sensitivity, specificity, positive and negative predictive value of 94.2%, 30.2%, 68.4% and 76.5%, respectively. The MTBDRplus assay detected 16.8% (16/95) of the isolates as drug resistant and MGIT detected a comparable number 15.9% (11/69) as resistant. Cohen's kappa value showed almost a perfect agreement between the two testing methods in detecting rifampicin (sensitivity 100% and specificity 98.3%) and multi-drug resistance (sensitivity 83.3% and specificity 100%). Spoligotyping identified 76.5% (13/17) of the drug resistant isolates as Euro-American and family 33 as the predominant family. Significant association was observed between drug resistant isolates and the dominant strains (χ2: 34.861; p = 0.040). Majority of the patients heard about tuberculosis as compared to drug resistant Mycobacterium. Conclusion Higher magnitude of drug resistance was found in the study area. The GenoTypic MDRTBplus assay had an acceptable drug sensitivity testing performance. Patients’ awareness towards the disease specifically to the resistant Mycobacterium was low.

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“…tuberculosis complex isolated followed by M . africanum accounting for 73% to 97.6% and 2.4% to 23% of samples respectively [42–45]. M .…”

Section: Discussionmentioning

confidence: 99%

Extra-pulmonary tuberculosis: A retrospective study of patients in Accra, Ghana

et al. 2019

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BackgroundInformation on extrapulmonary TB (EPTB) patients is limited in many African countries including Ghana. The study objective was to describe the epidemiology of EPTB patients diagnosed from different categories of health facilities in Accra, Ghana compared to pulmonary TB (PTB) patients and identify risk factors for mortality among EPTB patients.MethodWe conducted retrospective analyses of demographic and clinical data accessed from medical records of EPTB and PTB patients from different types of health facilities from June 2010 to December 2013. Factors at diagnosis associated with EPTB compared to pulmonary TB (PTB) and factors associated with treatment outcome death among EPTB patients were assessed using logistic regression.ResultsOut of 3,342 new TB patients ≥15 years diagnosed, 728 (21.8%) had EPTB with a male: female ratio of 1.17. The EPTB sites commonly affected were disseminated 32.8%, pleura 21%, spine 13%, and Central Nervous System (CNS) 11%. Treatment success rate for EPTB was 70.1% compared to 84.2% for PTB (p<0.001). In logistic regression, HIV positivity (adjusted Odds Ratio [aOR] 3.19; 95% confidence interval [CI] 2.69–3.79) and female gender (aOR 1.59; 95% CI 1.35–1.88) were found to be significantly associated with EPTB compared with PTB. Older age, being HIV positive (aOR 3.15; 95% CI 1.20–8.25) and having CNS TB (aOR 3.88; 95% CI 1.14–13.23) were associated with mortality among EPTB patients. While more EPTB patients were diagnosed in the tertiary hospital, health facility type was not associated with mortality.ConclusionEPTB patients in Accra have a worse treatment outcome compared to PTB patients with mortality of EPTB being associated with HIV, older age and CNS TB. Being HIV positive and female gender were found to be significantly associated with EPTB. Increased awareness of these factors may facilitate early case finding and better management outcomes for these patients.

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Genotyping and drug susceptibility testing of mycobacterial isolates from population-based tuberculosis prevalence survey in Ghana

Cited by 25 publications

References 16 publications

Occurrence of mutations associated with rifampicin and isoniazid resistant in Mycobacterium tuberculosis isolates from patients in Burkina Faso

Occurrence of mutations associated with rifampicin and isoniazid resistant in Mycobacterium tuberculosis isolates from patients in Burkina Faso

Phenotypic and genotypic drug sensitivity profiles of Mycobacterium tuberculosis infection and associated factors in northeastern Ethiopia

Extra-pulmonary tuberculosis: A retrospective study of patients in Accra, Ghana

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