Genotyping and antifungal susceptibility of Candida albicans strains from patients with vulvovaginal and cutaneous candidiasis in Palestine

Ali‐Shtayeh, Mohammed S.; Jamous, Rana M.; Alothman, Nihad H. A.; Baker, Moatasem H. Abu; Zaitoun, Salam Yousef Abu; Mallah, Omar Y.; Jamous, Rania M.

doi:10.5897/ajmr2014.7350

Cited by 4 publications

(3 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, in patients with RVVC, we found a higher prevalence of C. tropicalis in this study. Genotyping results showed that genotype A was the most common followed by genotype B then genotype C. This ranking agrees well with several other studies (6, 7), but differs from one study in which a higher incidence of genotype C was found (9). None of the PCR products corresponded to C. dubliniensis, and to our knowledge no C. dubliniensis has been reported in our district, but further research is required to confirm its absence.…”

Section: Discussionsupporting

confidence: 76%

“…In agreement with one study, the MICs of the intronless genotype A were significantly higher than those of the other two genotypes for 5-fluorocytosine and itraconazole (7). We also found genotype A to be significantly resistant against itraconazole, which is in agreement with two other studies in which genotype A exhibited more resistance than the other two genotypes (6,8,9). In contrast, other studies have found no association between antifungal resistance and genotype (7,23).…”

Section: Discussionsupporting

confidence: 65%

“…These genotypes have been reported to correlate with antifungal susceptibility (7)(8)(9) and virulence factors like cell adherence, phospholipase activity, and invasiveness (2,10,11). Genotyping based on group I introns has been used several times for studying the genetic variation of isolates involved in vulvovaginal candidiasis, cutaneous candidiasis, and oral candidiasis.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Genotypes and Susceptibility of Candida Species Causing Acute and Recurrent Vulvovaginitis

Manaen

Ling

2017

Jundishapur J Microbiol

View full text Add to dashboard Cite

Background: Candida albicans genotypes derived from PCR-based techniques targeting 25S rDNA have been shown to correlate with antifungal susceptibility and virulence factors. However, this method has not been used in isolates recovered from recurrent vulvovaginal candidiasis. Comparing intron-based genotypes with the drug susceptibility of isolates recovered from recurrent vulvovaginitis may aid in epidemiological studies and better patient management. Objectives: The purpose of this study was to investigate the genetic diversity of isolates involved in vulvovaginal candidiasis and their susceptibility to seven antifungal agents. Methods: Candida genotyping based on the presence or absence of a group I intron by PCR targeting 25S rDNA and drug susceptibility assays using a modified Alamar Blue broth microdilution method were carried out. Fisher's exact test and the Mann-Whitney U test were performed, and P < 0.05 was considered statistically significant. Results: Candida albicans was the predominant species among 384 Candida isolates followed by C. glabrata in acute and C. tropicalis in recurrent vulvovaginitis. Candida albicans from acute infections were more susceptible to fluconazole than isolates from recurrent cases but more resistant to miconazole and amphotericin B. The dominant genotype A was resistant to itraconazole and demonstrated higher minimal inhibitory concentrations (MICs) to 5-fluorocytosine and itraconazole. All C. albicans and nonalbicans species. with the group I intron were susceptible to 5-fluorocytosine. Conclusions: Candida albicans is the predominant cause of acute and recurrent vulvovaginal candidiasis, and drug susceptibility differed significantly among type of infection. Strain differences based on the group I intron may not be clinically significant in disease recurrence. However, the presence or absence of the group I intron has significant impact on antifungal MICs and resistance. Moreover, the group I intron in C. albicans and non-albicans may be the most important factor related to 5-fluorocytosine susceptibility.

show abstract

Section: Discussionsupporting

confidence: 76%

Section: Discussionsupporting

confidence: 65%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Genotypes and Susceptibility of Candida Species Causing Acute and Recurrent Vulvovaginitis

Manaen

Ling

2017

Jundishapur J Microbiol

View full text Add to dashboard Cite

show abstract

25S rDNA genotype and antifungal susceptibility of clinical Candida albicans in Ouagadougou (Burkina Faso)

et al. 2017

View full text Add to dashboard Cite

The present investigation aimed to assess the prevalence of genotypes of C. albicans in Ouagadougou and to analyze the relationship between the genetic diversity and antifungal susceptibility. Ninety-six clinical strains are included. They were diagnosed as C. albicans using germ tube test, chlamydospore formation, and Api-Candida test. Genotyping was performed using PCR targeting 25S rDNA. Antifungal susceptibilities were tested based on the disk diffusion method. The genotypes A (85.4%) was predominant followed by genotypes B (10.4%) and C (4.2%). The highest resistance rate with genotype A was obtained with fluconazole (74.4%). A resistance to amphotericin B was observed with genotypes A (29.3%) and B (30%).

show abstract

Genotypic diversity and antifungal susceptibility pattern of Candida albicans species isolated from hospitalized paediatric patients with urinary tract infection in Iran

et al. 2021

View full text Add to dashboard Cite

Aims: The present study aimed to determine the microsatellite length polymorphism (MLP) genotypic patterns and antifungal susceptibility profiles of Candida albicans isolated from patients with candiduria. Methods and Results: DNA of 50 C. albicans isolates was used for molecular identification based on the ITS 1 -5.8s-ITS 2 region. MLP assays were performed to amplify three loci (EF3, CDC3 and HIS3), and PCR products were used for fragment analysis. Antifungal susceptibility tests were performed according to CLSI M27 4th ed guidelines. In all, 38 different genotypes were detected with the three polymorphic loci among C. albicans isolates, and only one genotype was homozygous. In comparison to other countries, our genotypes were divided into three clusters, two of which were linked to France and a third of which was linked to Austria. The genetic structures of the 50 C. albicans isolates revealed varied heterozygosity and significant Hardy-Weinberg equilibrium at the EF3 locus. Only one (2%) and four (8%) of isolates showed resistance to fluconazole and itraconazole, respectively. In C. albicans genotype G25, one (2%) of the isolates showed cross-resistance and non-wild-type resistance to posaconazole, itraconazole and fluconazole. Conclusion: MLP typing is a useful tool to analyse the genetic structure of C. albicans isolates. High genetic diversity (38 genotypes) was detected in the isolates tested here. Compared to isolates in other countries, the ones from our patients had a clear relationship with French and Austrian isolates. Significance and Impact of the Study: Iranian isolates of C. albicans have a distinct genotype and show similarities only with French and Austrian isolates.

show abstract

Genotyping and antifungal susceptibility of Candida albicans strains from patients with vulvovaginal and cutaneous candidiasis in Palestine

Cited by 4 publications

References 25 publications

Genotypes and Susceptibility of Candida Species Causing Acute and Recurrent Vulvovaginitis

Genotypes and Susceptibility of Candida Species Causing Acute and Recurrent Vulvovaginitis

25S rDNA genotype and antifungal susceptibility of clinical Candida albicans in Ouagadougou (Burkina Faso)

Genotypic diversity and antifungal susceptibility pattern of Candida albicans species isolated from hospitalized paediatric patients with urinary tract infection in Iran

Contact Info

Product

Resources

About