Genotypic Variation among Six Isolates of Lactate Dehydrogenase-elevating Virus

Brinton, Margo A.; Gavin, Edith I.; Fernandez, Annabellee V.

doi:10.1099/0022-1317-67-12-2673

Cited by 22 publications

(12 citation statements)

References 24 publications

(32 reference statements)

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“…Another feature regularly encountered was the presence oflighter spots. These may be due to partial digestion (Pedersen & Haseltine, 1980), digestion with contaminating RNases, or random contamination with other RNA (Brinton et al, 1986). For example, oligonucleotide 67 (Fig.…”

Section: Resultsmentioning

confidence: 99%

Genetic Variation of Murray Valley Encephalitis Virus

Coelen

Mackenzie

1988

Journal of General Virology

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Section: Resultsmentioning

confidence: 99%

Genetic Variation of Murray Valley Encephalitis Virus

Coelen

Mackenzie

1988

Journal of General Virology

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“…The families Arteriviridae, Coronaviridae and Roniviridae make up the order Nidovirales. The arterivirus genome is a polycistronic, single-stranded RNA of positive polarity with a 5 type I cap (Sagripanti et al, 1986), and a 3 poly(A) tract (Brinton et al, 1986;Contag et al, 1986;Sagripanti, 1985;van Berlo et al, 1982). Arterivirus genomes range in length from 12.7 to 15.7 kb with SHFV being the longest (accession numbers: EAV, NC002532; LDV, NC002534; PRRSV, NC001961; SHFV, NC003092).…”

Section: Introductionmentioning

confidence: 99%

Two cellular proteins that interact with a stem loop in the simian hemorrhagic fever virus 3′(+)NCR RNA

et al. 2005

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Both full-length and subgenomic negative-strand RNAs are initiated at the 3' terminus of the positive-strand genomic RNA of the arterivirus, simian hemorrhagic fever virus (SHFV). The SHFV 3'(+) non-coding region (NCR) is 76 nts in length and forms a stem loop (SL) structure that was confirmed by ribonuclease structure probing. Two cell proteins, p56 and p42, bound specifically to a probe consisting of the SHFV 3'(+)NCR RNA. The 3'(+)NCR RNAs of two additional members of the arterivirus genus specifically interacted with two cell proteins of the same size. p56 was identified as polypyrimidine tract-binding protein (PTB) and p42 was identified as fructose bisphosphate aldolase A. PTB binding sites were mapped to a terminal loop and to a bulged region of the SHFV 3'SL structure. Deletion of either of the PTB binding sites in the viral RNA significantly reduced PTB binding activity, suggesting that both sites are required for efficient binding of this protein. Changes in the top portion of the SHFV 3'SL structure eliminated aldolase binding, suggesting that the binding site for this protein is located near the top of the SL. These cell proteins may play roles in regulating the functions of the genomic 3' NCR.

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“…The genome terminates at the 5' end with a type 1 cap (Cleaves & Dubin, 1979) and with uridylic acid at the 3' end (Rice et al, 1985;Brinton et al, 1986). Genomic RNA is organized into a small non-coding region (approximately 100 nucleotides) at the 5' terminus, followed by a single open reading frame (about 10-3 kb) which encodes the structural and non-structural proteins.…”

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confidence: 99%