Genotypic Variability in Differential Expression of lea2 and lea3 Genes and Proteins in Response to Salinity Stress in Fingermillet (Eleusine coracanaGaertn) and Rice (Oryza sativaL.) Seedlings

Jayaprakash, T. L.; Ramamohan, G.; Krishnaprasad, B. T.; Ganeshkumar,; Prasad, T. G.; Mathew, M. K.; Udayakumar, M.

doi:10.1006/anbo.1998.0712

Cited by 34 publications

(15 citation statements)

References 23 publications

(36 reference statements)

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“…The genotypic variability in differential expression of lea genes and proteins in response to salinity stress was noticed by Jayaprakash et al (1998) in finger millet (Eleusine coracana) and rice (Oryza sativa L.) seedlings. In analogy, in our present study the LEA 3 expression was found higher in both mulberry cultivars subjected to salinity stress treatments, besides there is a marked difference in the expression levels of LEA 3 in the two genotypes with higher level of expression in S1 than in ATP (Fig.…”

Section: ⎯⎯⎯⎯mentioning

confidence: 99%

Differential expression of LEA proteins in two genotypes of mulberry under salinity

Jyothsnakumari¹,

Thippeswamy²,

Veeranagamallaiah³

et al. 2009

Biol. plant.

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The relative water content (RWC), cell membrane integrity, protein pattern and the expression of late embryogenesis abundant proteins (LEA; group 1, 2, 3 and 4) under different levels of salt stress (0, 1.0, 1.5 and 2.0 % NaCl) were investigated in mulberry (Morus alba L.) cultivars (S1 and ATP) with contrasting salt tolerance. RWC and membrane integrity decreased with increase in NaCl concentration more in cv. ATP than in cv. S1. SDS-PAGE protein profile of mulberry leaves after the NaCl treatments showed a significant increase in 35, 41, 45 and 70 kDa proteins and significant decrease in 14.3, 18, 23, 28, 30, 42, 47 and 65 kDa proteins. Exposure of plants to NaCl resulted in higher accumulation of LEA proteins in S1 than ATP. The maximum content of LEA (group 3 and 4) was detected in S1 at 2.0 % NaCl, which correlates with its salt tolerance.

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Section: ⎯⎯⎯⎯mentioning

confidence: 99%

Differential expression of LEA proteins in two genotypes of mulberry under salinity

Jyothsnakumari¹,

Thippeswamy²,

Veeranagamallaiah³

et al. 2009

Biol. plant.

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“…DHNs are localized in various subcellular compartments, including cytosol (Roberts et al, 1993), nucleus (Houde et al, 1995), chloroplast (Artus et al, 1996), vacuole (Heyen et al, 2002), and proximal to the plasma membrane and protein bodies (Asghar et al, 1994;Egerton-Warburton et al, 1997;Puhakainen et al, 2004). Elevated expression of Dhn genes generally has been correlated with the acquisition of tolerance to abiotic stresses such as drought (Whitsitt et al, 1997), salt (Godoy et al, 1994;Jayaprakash et al, 1998), chilling (Ismail et al, 1999a), or freezing (Houde et al, 1995;Danyluk et al, 1998;Fowler et al, 2001). The differences in expression and tissue location suggest that individual members of the Dhn multigene family have somewhat distinct biological functions (Close, 1997;Zhu et al, 2000;Nylander et al, 2001).…”

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confidence: 99%

The K-Segment of Maize DHN1 Mediates Binding to Anionic Phospholipid Vesicles and Concomitant Structural Changes

et al. 2009

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Dehydrins (DHNs; late embryogenesis abundant D11 family) are a family of intrinsically unstructured plant proteins that accumulate in the late stages of seed development and in vegetative tissues subjected to water deficit, salinity, low temperature, or abscisic acid treatment. We demonstrated previously that maize (Zea mays) DHNs bind preferentially to anionic phospholipid vesicles; this binding is accompanied by an increase in α-helicity of the protein, and adoption of α-helicity can be induced by sodium dodecyl sulfate. All DHNs contain at least one “K-segment,” a lysine-rich 15-amino acid consensus sequence. The K-segment is predicted to form a class A2 amphipathic α-helix, a structural element known to interact with membranes and proteins. Here, three K-segment deletion proteins of maize DHN1 were produced. Lipid vesicle-binding assays revealed that the K-segment is required for binding to anionic phospholipid vesicles, and adoption of α-helicity of the K-segment accounts for most of the conformational change of DHNs upon binding to anionic phospholipid vesicles or sodium dodecyl sulfate. The adoption of structure may help stabilize cellular components, including membranes, under stress conditions.

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“…Several other studies in different species demonstrated that upon acclimation in seedlings as well as plants, significant increase in HSPs (HSP 70, HSP 104, HSP 90 and HSP 18.1) occurred (Uma et al 1995;Kumar et al 1999;Srikanthbabu et al 2002;Senthil-Kumar et al 2003). The importance of acquired thermotolerence in crop plants under heat stress was reported by several authors (Uma et al 1995;Jayaprakash et al 1998;Kumar et al 1999;Burke et al 2000;Burke 2001;Srikanthbabu et al 2002;Senthil-Kumar et al 2003;2007).…”

Section: Introductionmentioning

confidence: 85%

Heat stress screening of peanut (Arachis hypogaea L.) seedlings for acquired thermotolerance

Selvaraj

Burow²,

Burke³

et al. 2011

Plant Growth Regul

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Heat can be one of the major abiotic stresses that adversely affect crop production worldwide at different stages of development. As field screening for heat tolerance can be inconsistent and seasonally-limited, it is important to develop a reliable protocol under controlled conditions that allows simultaneous screening of multiple genotypes. The objective of this research was to develop a straightforward laboratory protocol using acquired thermotolerance (ATT) in peanut seedlings as a measure of one mechanism of heat stress tolerance. Sixteen genotypes, including selected accessions of the US peanut minicore collection along with standard checks, were evaluated for acquired themotolerance in two independent experiments. A change in the temperature sensitivity of chlorophyll accumulation was used as an indicator of acquired thermotolerance. Pre-incubation at 38°C for 4 h before the 30-min 50°C challenge triggered the acquired thermotolerance system of the leaf disks, resulting in chlorophyll accumulation upon exposure to light. There was considerable variation among genotypes for ATT in both experiments. Genotypic ranking for mean ATT values were highly correlated (0.949) in both experiments. The effect of seed weight on ATT was not significant. This method is relatively simple and inexpensive and can be used to screen a large number of genotypes.

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Genotypic Variability in Differential Expression of lea2 and lea3 Genes and Proteins in Response to Salinity Stress in Fingermillet (Eleusine coracanaGaertn) and Rice (Oryza sativaL.) Seedlings

Cited by 34 publications

References 23 publications

Differential expression of LEA proteins in two genotypes of mulberry under salinity

Differential expression of LEA proteins in two genotypes of mulberry under salinity

The K-Segment of Maize DHN1 Mediates Binding to Anionic Phospholipid Vesicles and Concomitant Structural Changes

Heat stress screening of peanut (Arachis hypogaea L.) seedlings for acquired thermotolerance

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