Genotypic to Expression Profiling of Bovine Calcium Channel, Voltage-Dependent, Alpha-2/Delta Subunit 1 Gene, and Their Association with Bovine Mastitis Among Frieswal (HFX Sahiwal) Crossbred Cattle of Indian Origin

Deb, Rajib; Singh, Umesh; Kumar, Sushil; Kumar, Arun; Singh, Rani; Sengar, Gyanendra Singh; Mann, Sandeep; Sharma, Arjava

doi:10.1080/10495398.2013.836106

Cited by 12 publications

(5 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our examination of the cellular calcium flux suggests that MA, but not LA, recognizes FFAR1/GPR40 in BUVECs. The CACNA2D1 gene encodes the alpha 2/delta subunit which is an accessory part of the voltage-gated calcium channel family (e.g., LTCC) and considered to be an important candidate protein influencing bovine mastitis [ 35 ]. Although many publications have reported LTCC activity in bovine immune cells, there is not yet any evidence that these channels are expressed in bovine endothelial cells.…”

Section: Discussionmentioning

confidence: 99%

Differential intracellular calcium influx, nitric oxide production, ICAM-1 and IL8 expression in primary bovine endothelial cells exposed to nonesterified fatty acids

et al. 2016

View full text Add to dashboard Cite

BackgroundNonesterified fatty acids (NEFAs) are involved in proinflammatory processes in cattle, including in the increased expression of adhesion molecules in endothelial cells. However, the mechanisms underlying these effects are still unknown. The aim of this study was to assess the effects of NEFAs on the intracellular calcium (Ca2+i) influx, nitric oxide production, and ICAM-1 and IL-8 expression in primary bovine umbilical vein endothelial cells (BUVECs).ResultsMyristic (MA), palmitic (PA), stearic (SA), oleic (OA) and linoleic acid (LA) rapidly increased Ca2+i. The calcium response to all tested NEFAs showed an extracellular calcium dependence and only the LA response was significantly inhibited until the intracellular calcium was chelated. The EC50 values for MA and LA were 125 μM and 37 μM, respectively, and the MA and LA effects were dependent on calcium release from the endoplasmic reticulum stores and on the L-type calcium channels. Only the calcium response to MA was significantly reduced by GW1100, a selective G-protein-coupled free fatty acid receptor (GPR40) antagonist. We also detected a functional FFAR1/GPR40 protein in BUVECs by using western blotting and the FFAR1/GPR40 agonist TAK-875. Only LA increased the cellular nitric oxide levels in a calcium-dependent manner. LA stimulation but not MA stimulation increased ICAM-1 and IL-8-expression in BUVECs. This effect was inhibited by GW1100, an antagonist of FFAR1/GPR40, but not by U-73122, a phospholipase C inhibitor.ConclusionsThese findings strongly suggest that each individual NEFA stimulates endothelial cells in a different way, with clearly different effects on intracellular calcium mobilization, NO production, and IL-8 and ICAM-1 expression in primary BUVECs. These findings not only extend our understanding of NEFA-mediated diseases in ruminants, but also provide new insight into the different molecular mechanisms involved during endothelial cell activation by NEFAs.Electronic supplementary materialThe online version of this article (doi:10.1186/s12917-016-0654-3) contains supplementary material, which is available to authorized users.

show abstract

Section: Discussionmentioning

confidence: 99%

Differential intracellular calcium influx, nitric oxide production, ICAM-1 and IL8 expression in primary bovine endothelial cells exposed to nonesterified fatty acids

et al. 2016

View full text Add to dashboard Cite

show abstract

“…Deb et al . [ 45 ] from their study of SNP detection by RT-PCR in bovine CACNA2D1 gene (chromosome 4) at G519663A locus, demonstrated that GG genotype is associated with the lowest somatic cell score (SCS), and the transcript abundance of CACNA2D1 mRNA and was favorable for the mastitis resistance. Similar finding with the help of PCR-RFLP was observed by Yuan et al .…”

Section: Immuno-pathogenesismentioning

confidence: 99%

Molecular markers for resistance against infectious diseases of economic importance

et al. 2017

View full text Add to dashboard Cite

Huge livestock population of India is under threat by a large number of endemic infectious (bacterial, viral, and parasitic) diseases. These diseases are associated with high rates of morbidity and mortality, particularly in exotic and crossbred cattle. Beside morbidity and mortality, economic losses by these diseases occur through reduced fertility, production losses, etc. Some of the major infectious diseases which have great economic impact on Indian dairy industries are tuberculosis (TB), Johne’s disease (JD), mastitis, tick and tick-borne diseases (TTBDs), foot and mouth disease, etc. The development of effective strategies for the assessment and control of infectious diseases requires a better understanding of pathogen biology, host immune response, and diseases pathogenesis as well as the identification of the associated biomarkers. Indigenous cattle (Bos indicus) are reported to be comparatively less affected than exotic and crossbred cattle. However, genetic basis of resistance in indigenous cattle is not well documented. The association studies of few of the genes associated with various diseases, namely, solute carrier family 11 member 1, Toll-like receptors 1, with TB; Caspase associated recruitment domain 15, SP110 with JD; CACNA2D1, CD14 with mastitis and interferon gamma, BoLA-DRB3.2 alleles with TTBDs, etc., are presented. Breeding for genetic resistance is one of the promising ways to control the infectious diseases. High host resistance is the most important method for controlling such diseases, but till today no breed is total immune. Therefore, work may be undertaken under the hypothesis that the different susceptibility to these diseases are exhibited by indigenous and crossbred cattle is due to breed-specific differences in the dealing of infected cells with other immune cells, which ultimately influence the immune response responded against infections. Achieving maximum resistance to these diseases is the ultimate goal, is technically possible to achieve, and is permanent. Progress could be enhanced through introgression of resistance genes to breeds with low resistance. The quest for knowledge of the genetic basis for infectious diseases in indigenous livestock is strongly warranted.

show abstract

“…Calcium channel, voltage-dependent, alpha-2/delta subunit 1 residing on chromosome 4, containing 42 exons, is another key genetic signature with an important role in clinical mastitis. It has been documented that SNP-G519663A at exon 18 (80), SNP-G519663A, and SNP-A526745G at exon 24 in the bovine CACNA2D1 gene were significantly linked with lower milk SCS levels and mastitis resistance in dairy cattle (82, 102). Moreover, it has also been noticed that SNP G38819398A at exon 18 (81), variant-T38826986G at exon 19 (103), and SNP-C367284A of CACNA2D1 (104) were positively linked with milk SCS in Sahiwal and Karan Fries.…”

Section: Calcium Channel Voltage-dependent Alpha-2/delta Subunit 1 (C...mentioning

confidence: 99%

Genetic polymorphisms in immune- and inflammation-associated genes and their association with bovine mastitis resistance/susceptibility

Khan

Wang

et al. 2023

Front. Immunol.

View full text Add to dashboard Cite

Bovine mastitis, the inflammation of the mammary gland, is a contagious disease characterized by chemical and physical changes in milk and pathological changes in udder tissues. Depressed immunity and higher expression of inflammatory cytokines with an elevated milk somatic cell count can be observed during mastitis in dairy cattle. The use of somatic cell count (SCC) and somatic cell score (SCS) as correlated traits in the indirect selection of animals against mastitis resistance is in progress globally. Traditional breeding for mastitis resistance seems difficult because of the low heritability (0.10-0.16) of SCC/SCS and clinical mastitis. Thus, genetic-marker-selective breeding to improve host genetics has attracted considerable attention worldwide. Moreover, genomic selection has been found to be an effective and fast method of screening for dairy cattle that are genetically resistant and susceptible to mastitis at a very early age. The current review discusses and summarizes the candidate gene approach using polymorphisms in immune- and inflammation-linked genes (CD4, CD14, CD46, TRAPPC9, JAK2, Tf, Lf, TLRs, CXCL8, CXCR1, CXCR2, C4A, C5, MASP2, MBL1, MBL2, LBP, NCF1, NCF4, MASP2, A2M, and CLU, etc.) and their related signaling pathways (Staphylococcus aureus infection signaling, Toll-like receptor signaling, NF-kappa B signaling pathway, Cytokine-cytokine receptor, and Complement and coagulation cascades, etc.) associated with mastitis resistance and susceptibility phenotypic traits (IL-6, interferon-gamma (IFN-γ), IL17, IL8, SCS, and SCC) in dairy cattle.

show abstract

Genotypic to Expression Profiling of Bovine Calcium Channel, Voltage-Dependent, Alpha-2/Delta Subunit 1 Gene, and Their Association with Bovine Mastitis Among Frieswal (HFX Sahiwal) Crossbred Cattle of Indian Origin

Cited by 12 publications

References 21 publications

Differential intracellular calcium influx, nitric oxide production, ICAM-1 and IL8 expression in primary bovine endothelial cells exposed to nonesterified fatty acids

Differential intracellular calcium influx, nitric oxide production, ICAM-1 and IL8 expression in primary bovine endothelial cells exposed to nonesterified fatty acids

Molecular markers for resistance against infectious diseases of economic importance

Genetic polymorphisms in immune- and inflammation-associated genes and their association with bovine mastitis resistance/susceptibility

Contact Info

Product

Resources

About