Genotypic resistance in plasma and peripheral blood lymphocytes in a group of naive HIV-1 patients

Bon, Isabella; Gibellini, Davide; Borderi, Marco; Alessandrini, Federica; Vitone, Francesca; Schiavone, Pasqua; Re, Maria Carla

doi:10.1016/j.jcv.2006.12.018

Cited by 34 publications

(27 citation statements)

References 39 publications

(43 reference statements)

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“…In the samples from the TE patients, the majority of DRMs appeared in viral RNA (99%) whereas those from the TN patients displayed a significantly higher proportion of DRMs in proviral DNA (33%). These data are in line with other published studies investigating information on resistance in viral RNA and proviral DNA of identical blood samples [17,18,19,20,21,22]. …”

Section: Discussionsupporting

confidence: 91%

Proviral DNA as a Target for HIV-1 Resistance Analysis

Lübke

Cristanziano²,

Sierra³

et al. 2015

Intervirology

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Background: Resistance analysis from viral RNA is restricted to detectable viral load. Therefore, analysis from proviral DNA could help in cases with low-level or suppressed viremia. Methods: Viral plasma RNA and the corresponding cellular proviral DNA of 78 EDTA samples from 48 therapy-naïve (TN) and 30 therapy-experienced (TE) HIV-1-infected patients were isolated and analyzed for their resistance profiles in the protease and reverse transcriptase genes. Results: Overall, 175 drug-resistance mutations (DRMs) were detected in 25/30 TE (83.3%) and 5/48 TN (10.4%) samples. The TE patients displayed a mean number of 6.68 DRMs in RNA and 5.20 in DNA. In the TN patients, a mean of 0.8 DRMs was found in RNA and 1.0 in DNA; 75% of the DRMs were detected in RNA and DNA simultaneously. In the TE samples, 76% of the DRMs were detected simultaneously in RNA and DNA, 23% exclusively in RNA and 1% in DNA only. The TN samples revealed a significantly higher frequency of DRMs in DNA than in RNA. Conclusions: Proviral DNA resistance testing provides additional resistance information for TN patients. It is also a reliable alternative for TE patients with unsuccessful RNA testing and can provide valuable information when no records are available.

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Section: Discussionsupporting

confidence: 91%

Proviral DNA as a Target for HIV-1 Resistance Analysis

Lübke

Cristanziano²,

Sierra³

et al. 2015

Intervirology

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“…Recently, the results of a study aimed at comparing cell-free and cell-associated resistance have been published (2). The study, conducted with 31 naive patients, showed that direct sequencing of DNA provirus disclosed key mutations in samples from seven patients and that five of these mutations were not detected by routine plasma analysis.…”

Section: Discussionmentioning

confidence: 99%

Both Human Immunodeficiency Virus Cellular DNA Sequencing and Plasma RNA Sequencing Are Useful for Detection of Drug Resistance Mutations in Blood Samples from Antiretroviral-Drug-Naive Patients

et al. 2007

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“…The study with higher levels of resistance was based on proviral DNA (Sucupira et al 2007), whereas most of the other studies were based on HIV-1 virion RNA. Few studies have compared plasma and cell mutation profiles, but cell/plasma discordance is not uncommon (Bon et al 2007) and one recent study suggested an astonishingly high degree of heterogeneity among peripheral blood mononuclear cells (PBMCs) in regards to resistance (Quan et al 2008). On the other hand, at least one study reporting a low primary resistance was also performed using the cell provirus (EyerSilva et al 2008).…”

Section: Discussionmentioning

confidence: 99%

Molecular characterisation of newly identified HIV-1 infections in Curitiba, Brazil: preponderance of clade C among males with recent infections

Ferreira

Thomaz

Rodrigues

et al. 2008

Mem. Inst. Oswaldo Cruz

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The Research Capacity ProgramAs in many areas of Brazil, the AIDS epidemic in Curitiba is relatively stable, but surveillance is important to support public policy. The molecular characteristics of HIV may be instrumental for monitoring epidemic trends. We evaluated plasma HIV-1 RNA (n = 37) from 38 cases presenting with positive serology, who were among 820 consenting volunteers visiting the downtown counselling and serology testing centre. Seroprevalence was 4.6% 3) and the estimated HIV incidence, as defined by the BED assay, was 2.86 persons/years ). An additional set of contemporaneous, anonymous samples from a local laboratory was also analysed (n = 20) . Regions of the HIV-1 polymerase (n = 57) and envelope (n = 34) were evaluated for subtyping, determination of mosaic structure, primary drug resistance mutations (pDRM), envelope V3 loop motifs and amino acid signatures related to viral tropism. HIV-1 clade B was observed in 53% of cases; HIV-1C in 30% and BC mosaics in 14%, with one F genome and one CF mosaic. Clade C infection was associated with recent infections among males (p < 0.03). Stanford surveillance pDRM was observed in 8.8% of sequences, with 7% showing high level resistance to at least one antiretroviral drug. Tropism for CXCR4 co-receptor was predicted in 18% of envelope sequences, which were exclusively among clade B genomes and cases with serological reactivity to chronic infection.Key words: epidemiology -antiretroviral resistance -genetic diversity -HIV-1 -tropism -BrazilUnderstanding the local HIV-1 epidemic will not only support the regional response, but it may also provide information to nationwide efforts in the fight against AIDS. The variability of HIV-1 has been an obstacle to treatment and to the development of prevention innovations such as vaccines. HIV-1 clusters phylogenetically into distinct clades (subtypes), a fact that has helped to unravel the molecular characteristics of the epidemic. In some regions, differential distribution of HIV-1 clades has been documented (Van Harmelen et al. 1997, Tovanabutra 2001, Rios et al. 2005, Ryan et al. 2007) and phylogeny may indicate the source(s) of incoming variants (Sarker 2008). Worldwide, clade C is responsible for about half of the epidemic, whereas clade B is the major variant in industrialised nations (Hemelaar et al. 2006). HIV variability may influence the performance of laboratory tools for diagnostic, monitoring and surveillance (Koch et al. 2001 (Baeten et al. 2007, Kaleebu 2007 and transmission potential (Yang et al. 2003, John-Stewart et al. 2005 have been associated to distinct HIV clades, but the actual impact of genetic diversity in HIV disease still remains uncertain (Stebbing & Moyle 2003, Hemelaar et al. 2006, Tebit et al. 2007). However, some characteristics of HIV, such as tropism to either CCR5 or CXCR4 coreceptors, seem to be associated with a distinct pattern of disease progression (Shepherd et al. 2008). Another relevant issue is the identification of primary drug resistance mutations (pDRM) among untreate...

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Genotypic resistance in plasma and peripheral blood lymphocytes in a group of naive HIV-1 patients

Cited by 34 publications

References 39 publications

Proviral DNA as a Target for HIV-1 Resistance Analysis

Proviral DNA as a Target for HIV-1 Resistance Analysis

Both Human Immunodeficiency Virus Cellular DNA Sequencing and Plasma RNA Sequencing Are Useful for Detection of Drug Resistance Mutations in Blood Samples from Antiretroviral-Drug-Naive Patients

Molecular characterisation of newly identified HIV-1 infections in Curitiba, Brazil: preponderance of clade C among males with recent infections

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