Genotypic Properties of the Entomopathogenic Fungus Nomuraea rileyi

Boucias, Drion G.; Tigano, Myrian S.; Sosa-Gómez, Daniel Ricardo; Glare, Travis R.; Inglis, Peter W.

doi:10.1006/bcon.2000.0857

Cited by 50 publications

(26 citation statements)

References 36 publications

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“…Majer et al, 1996;Mueller et al, 1996;Rosendahl and Taylor, 1997) including entomopathogenic taxa (e.g. Aquino de Muro et al, 2003;Boucias et al, 2000), to our knowledge it has not been applied to M. anisopliae. We found that the AFLP method provided highly reproducible results (resolution limit of 86%), and showed a high level of genotypic diversity (i.e.…”

Section: Discussionmentioning

confidence: 99%

Genetic diversity of Metarhizium anisopliae var. anisopliae in southwestern British Columbia

Inglis

Duke

Goettel

et al. 2008

Journal of Invertebrate Pathology

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Section: Discussionmentioning

confidence: 99%

Genetic diversity of Metarhizium anisopliae var. anisopliae in southwestern British Columbia

Inglis

Duke

Goettel

et al. 2008

Journal of Invertebrate Pathology

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“…Telomeric probes have previously been used for DNA fingerprinting of filamentous fungi such as U. maydis (Sanchez-Alonso et al 1996), Metarhizium sp. (Inglis et al 1999), Nomuraea rileyi (Boucias et al 2000), Cercospora caricis (Inglis et al 2001b) and Beauveria sp. (Viaud et al 1998).…”

Section: Discussionmentioning

confidence: 99%

Monomorphic subtelomeric DNA in the filamentous fungus, Metarhizium anisopliae,contains a RecQ helicase-like gene

et al. 2005

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In most filamentous fungi, telomere-associated sequences (TASs) are polymorphic, and the presence of restriction fragment length polymorphisms (RFLPs) may permit the number of chromosome ends to be estimated from the number of telomeric bands obtained by restriction digestion. Here, we describe strains of Metarhizium, Gliocladium and Paecilomyces species in which only one or a few telomeric bands of unequal intensity are detectable by Southern hybridization, indicating that interchromosomal TAS exchange occurs. We also studied an anomalous strain of Metarhizium anisopliae, which produces polymorphic telomeric bands larger than 8 kb upon digestion of genomic DNA with XhoI. In this case, the first XhoI site in from the chromosome end must lie beyond the presumed monomorphic region. Cloned telomeres from this strain comprise 18-26 TTAGGG repeats, followed at the internal end of the telomere tract by five repeats of the telomere-like sequence TAAACGCTGG. An 8.1-kb TAS clone also contains a gene for a RecQ-like helicase, designated TAH1, suggesting that this TAS is analogous to the Y' elements in yeast and the subtelomeric helicase ORFs of Ustilago maydis (UTASRecQ) and Magnaporthe grisea (TLH1). The TAS in the anomalous strain of M. anisopliae, however, appears distinct from these in that it is found at most telomeres and its predicted protein product possesses a significantly longer N-terminal region in comparison to the M. grisea and U. maydis helicases. Hybridization analyses showed that TAH1 homologues are present in all other anomalous M. anisopliae strains studied, as well as in some other polymorphic strains, where the recQ-like gene also appears to be telomere-associated.

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“…However AFLP (amplified fragment length polymorphism) might be a more reliable molecular marker technique for specific identification of B. bassiana as it encompasses the analysis of the total genome, and the amount of information obtained is extensive. The AFLP technique on agarose‐synergels has been used successfully to examine the interspecific variation within the invertebrate mycopathogen Nomuraea rileyi [17,18].…”

Section: Introductionmentioning

confidence: 99%

The use of amplified fragment length polymorphism for molecular analysis ofBeauveria bassianaisolates from Kenya and other countries, and their correlation with host and geographical origin

Muro

Mehta

Moore

2003

FEMS Microbiology Letters

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Beauveria spp. (one Beauveria sp., two B. brongniartii, and 47 B. bassiana) isolated from insects and soil from Kenya and other 16 different countries, were obtained from the CABI Bioscience Genetic Resource Collection. DNA was extracted from the 50 isolates and their genetic variability was investigated using restriction analysis of the internal-transcribed-spacer ribosomal region restriction fragment length polymorphism (ITS-RFLP), ITS sequencing, and amplified fragment length polymorphism (AFLP). The B. bassiana isolates could not be distinguished by ITS-RFLP as all of them showed the same banding pattern. However, the AFLP technique provided more information on polymorphism between the isolates, allowing them to be clustered by relative similarity using band matching and unweighted pair group method with arithmetic mean analysis. Although no significant correlation between the isolates and host and geographical origins were observed, the technique revealed clonal populations of B. bassiana within Kenya.

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Genotypic Properties of the Entomopathogenic Fungus Nomuraea rileyi

Cited by 50 publications

References 36 publications

Genetic diversity of Metarhizium anisopliae var. anisopliae in southwestern British Columbia

Genetic diversity of Metarhizium anisopliae var. anisopliae in southwestern British Columbia

Monomorphic subtelomeric DNA in the filamentous fungus, Metarhizium anisopliae,contains a RecQ helicase-like gene

The use of amplified fragment length polymorphism for molecular analysis ofBeauveria bassianaisolates from Kenya and other countries, and their correlation with host and geographical origin

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