Genotypic patterns of multidrug-resistant Acinetobacter baumannii: A systematic review

Rahimzadeh, Golnar; Rezai, Mohammad Sadegh; Farshidi, Fereshteh

doi:10.4103/abr.abr_434_22

Cited by 1 publication

(1 citation statement)

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“…According to CHINET statistics, the percentages of AB resistant to imipenem and meropenem in China have risen from 31.7 and 39.9% in 2006 to 71.5 and 72.3% in 2021, respectively ( Hu et al, 2018 ). Abroad, the prevalence of MDRAB in Iran increased from 50% in 2001–2007 to 74% in 2010–2015 ( Rezai et al, 2023 ). Previously, we identified blaOXA-23 as the dominant carbapenemase gene type in CRAB isolated from clinical patients with BSIs at our hospital from 2018 to 2022.…”

Section: Introductionmentioning

confidence: 99%

Development and clinical validation of a dual ddPCR assay for detecting carbapenem-resistant Acinetobacter baumannii in bloodstream infections

Kou,

Zhu,

Zhang

et al. 2024

Front. Microbiol.

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ObjectiveAcinetobacter baumannii (A. baumannii, AB) represents a major species of Gram-negative bacteria involved in bloodstream infections (BSIs) and shows a high capability of developing antibiotic resistance. Especially, carbapenem-resistant Acinetobacter baumannii (CRAB) becomes more and more prevalent in BSIs. Hence, a rapid and sensitive CRAB detection method is of urgent need to reduce the morbidity and mortality due to CRAB-associated BSIs.MethodsA dual droplet digital PCR (ddPCR) reaction system was designed for detecting the antibiotic resistance gene OXA-23 and AB-specific gene gltA. Then, the specificity of the primers and probes, limit of detection (LOD), linear range, and accuracy of the assay were evaluated. Furthermore, the established assay approach was validated on 37 clinical isolates and compared with blood culture and drug sensitivity tests.ResultsThe dual ddPCR method established in this study demonstrated strong primer and probe specificity, distinguishing CRAB among 21 common clinical pathogens. The method showed excellent precision (3 × 10−4 ng/μL, CV < 25%) and linearity (OXA-23: y = 1.4558x + 4.0981, R2 = 0.9976; gltA: y = 1.2716x + 3.6092, R2 = 0.9949). While the dual qPCR LOD is 3 × 10−3 ng/μL, the dual ddPCR’s LOD stands at 3 × 10−4 ng/μL, indicating a higher sensitivity in the latter. When applied to detect 35 patients with BSIs of AB, the results were consistent with clinical blood culture identification and drug sensitivity tests.ConclusionThe dual ddPCR detection method for OXA-23 and gltA developed in this study exhibits good specificity, excellent linearity, and a higher LOD than qPCR. It demonstrates reproducibility even for minute samples, making it suitable for rapid diagnosis and precision treatment of CRAB in BSIs.

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Section: Introductionmentioning

confidence: 99%