Genotypic diversity of Pasteurella multocida isolates from pigs and poultry in Australia

Abstract

Objective To investigate the genotype and diversity of Pasteurella multocida present in pig herds and to determine the extent of overlap with isolates from poultry flocks in Australia. Methods A total of 43 isolates from pigs from different farms and regions of Australia were used in this study. A diverse collection of 41 poultry isolates, with 31 being previously characterised, was also used. The pig isolates and 10 poultry isolates were identified by species‐specific PCR assay, serotyped by the Heddleston scheme and genotyped by a multiplex PCR based on the lipopolysaccharide (LPS) outer core biosynthesis locus, repetitive element PCR fingerprinting (rep‐PCR) and multilocus sequence typing (MLST), with the latter being used on a subset of the isolates based on the rep‐PCR results. Results Only 4 out of 8 recognised LPS genotypes were found in the pig isolates, with each isolate assigned to an LPS genotype. In contrast, 77% of the isolates were non‐typable or cross‐reacting in the Heddleston serotyping scheme. The rep‐PCR analysis recognised 20 patterns, yet only 16 sequence types (STs) were found and 4 were new STs. There were 5 STs (STs 7, 11, 20, 24 and 58) shared among the pig and poultry isolates. Conclusions Although only limited numbers of isolates have been examined, there is evidence of a sharing of genotypes between Australian pigs and chickens. These findings have major implication for biosecurity measures with regard to minimising both direct (e.g. animal to animal) and in‐direct (e.g. shared staff or cross‐visitors) contact between poultry and pigs.

Pathogens associated with pleuritic pig lungs at an abattoir in Queensland Australia

“…Similar findings have been reported in China, where P. multocida LPS types LPS 3 and 6 were the dominant genotypes associated with pig respiratory disorders 43 . A previous Australian study has also reported these to be the dominant LPS genotypes 44 . As these two genotypes have been retrieved from different mammals with different diseases, it has been suggested that LPS types L6 and L3 are the dominant pathogenic LPS genotypes for mammalian infections 45 …”

“…43 A previous Australian study has also reported these to be the dominant LPS genotypes. 44 As these two genotypes have been retrieved from different mammals with different diseases, it has been suggested that LPS types L6 and L3 are the dominant pathogenic LPS genotypes for mammalian infections. 45 Many of the P. multocida isolates were recovered in large numbers (plate score ≥3) and may have been a main contributor to the observed pleurisy.…”

Advances of intra‐species molecular typing analysis of aquatic probiotics approved by the Chinese Ministry of Agriculture

“…Commercial software (Bionumeric version 7.6, Applied Maths Inc.) is used to analyse the patterns. If two isolates have the same genomic fingerprint (an identical band pattern including size and intensity), they are assumed to be the same strain (Turni et al 2018). Moreover, to compare relatedness of strains, rep-PCR banding profiles are visually observed and consistently amplified fragments are scored for presence (1) or absence (0).…”

In this issue – October 2018: Mechanisms and treatment of tumour angiogenesis is · Coagulation times depend on methods used · Experimental studies on footrot control · Pasteurella multocida in pigs and poultry · Pomegranate poisoning in cattle · Outcome of foals with hypernatremia

“…The observation that there are sequence types (ST) of Pasteurella multocida are associated with both poultry and pigs is important because it has implications for biosecurity practices and allows an evaluation of the risk of cross‐species transmission based on the observed ST of isolates …”

“…The observation that there are sequence types (ST) of Pasteurella multocida are associated with both poultry and pigs is important because it has implications for biosecurity practices and allows an evaluation of the risk of cross-species transmission based on the observed ST of isolates. 5 Pasteurella multocida is an opportunist pathogen in pigs that is associated with porcine respiratory disease complex. Because this results from a combination of a range of viral or bacterial causes, and is associated with environmental stressors and challenges, the specific clinical signs of the disease are difficult to work out.…”