Genotypic Diversity of Acidovorax Strains Isolated from Activated Sludge and Description of Acidovorax defluvii sp. nov.

Verminephrobacter eiseniae gen. nov., sp. nov., a nephridial symbiont of the earthworm Eisenia foetida (Savigny)

“…DNA samples from A. temperans and A. delafieldii were used as controls. The DNA G+C content (determined for isolates EF01-1, EF01-2 T , EF02-2 and EF05-1) was 67.0±0.2 mol%, well within the 60-72 mol% values reported for other species of the genus Acidovorax (Schulze et al, 1999;Gardan et al, 2000Gardan et al, , 2003, and in close agreement with the 65.2 mol% observed for the genome sequence of isolate EF01-2 (unpublished observations).…”

“…reported for other species of the family Comamonadaceae was below 90 % (not shown; Willems et al, 1990Willems et al, , 1992Schulze et al, 1999). The profiles obtained in this study for the type strains of the reference Acidovorax species growing on ACMP plates and those reported in the literature for the corresponding species differed by less than 1 %.…”

“…Acidovorax-like 16S rRNA gene sequences are frequently recovered from wastewater treatment plants and other aquatic environments (Schweitzer et al, 2001;Hoshino et al, 2005;Manefield et al, 2005). The genus Acidovorax includes species isolated from soil and sludge (Willems et al, 1990;Schulze et al, 1999;Heylen et al, 2008), in addition to a group of plant pathogens with a varied host range (Willems et al, 1992;Gardan et al, 2000Gardan et al, , 2003. The present study reports the isolation and characterization of a relative of the genus Acidovorax from the nephridia of E. foetida.…”

“…The mean 16S rRNA gene sequence similarity between the earthworm symbionts and either of the other two groups within the genus Acidovorax (92.5±0.5 % and Willems et al, 1992);3, A. konjaci (n53;Willems et al, 1992);4, A. defluvii (n58;Schulze et al, 1999);5, A. delafieldii (n514;Willems et al, 1990);6, A. facilis (n54;Willems et al, 1990);7, A. temperans (n518;Willems et al, 1990);8, A. valerianellae (n519;Gardan et al, 2003). Catalase 2 (2) 93.3±0.3 % similarity to plant pathogens and other species within the genus, respectively) fell within the range observed at a genus rank (Konstantinidis & Tiedje, 2005b) and was well below that shared among other species in the genus.…”

Microbial Communities Involved in Methane Production from Coal Treated by Potassium Permanganate

“…Although acetoclastic and methylotrophic methanogens were also found, e.g. Methanosaeta [29] and Methanomethylovorans [30], they only consisted of approximately 4% of the sequence reads, suggesting the production of methane by mainly hydrogenotrophic methanogenesis [31][32][33][34][35][36][37][38].…”