Genotypic diversity and symbiotic effectiveness of rhizobia isolated from root nodules of Phaseolus vulgaris L. grown in Tunisian soils

Mhamdi, Ridha; Jebara, Moez; Aouani, Mohamed Elarbi; Ghrir, Rachid; Mars, Mohamed

doi:10.1007/s003740050499

Cited by 66 publications

(58 citation statements)

References 29 publications

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“…Rhizobium leguminosarum bv. phaseoli is commonly found in Europe, and it has also been reported to be present in Tunisia and Colombia (11,21,33,34). Rhizobium tropici is present in acid soils of South America (31) and has been found in France (2) and several African countries (4, 10).…”

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confidence: 99%

“…R. etli bv. phaseoli is found in regions where common bean has been introduced, such as Spain, France, Austria, Senegal, Gambia, and Tunisia (10,21,24,33,34,44). However, in these countries other Rhizobium species also nodulate this legume.…”

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confidence: 99%

“…Rhizobium giardinii has been found only in European and Tunisian soils (3,21,34). Rhizobium gallicum has been found nodulating beans in Europe (3,21) and Tunisia (33,34), and one Mexican strain (strain FL27) has been identified as a member of this species (44).…”

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Rhizobium etli and Rhizobium gallicum Nodulate Common Bean ( Phaseolus vulgaris ) in a Traditionally Managed Milpa Plot in Mexico: Population Genetics and Biogeographic Implications

Silva

Vinuesa

Eguiarte

et al. 2003

Appl Environ Microbiol

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The stability of the genetic structure of rhizobial populations nodulating Phaseolus vulgaris cultivated in a traditionally managed milpa plot in Mexico was studied over three consecutive years. The set of molecular markers analyzed (including partial rrs, glnII, nifH, and nodB sequences), along with host range experiments, placed the isolates examined in Rhizobium etli bv. phaseoli and Rhizobium gallicum bv. gallicum. Cluster analysis of multilocus enzyme electrophoresis and plasmid profile data separated the two species and identified numerically dominant clones within each of them. Population genetic analyses showed that there was high genetic differentiation between the two species and that there was low intrapopulation differentiation of the species over the 3 years. The results of linkage disequilibrium analyses are consistent with an epidemic genetic structure for both species, with frequent genetic exchange taking place within conspecific populations but not between the R. etli and R. gallicum populations. A subsample of isolates was selected and used for 16S ribosomal DNA PCR-restriction fragment length polymorphism analysis, nifH copy number determination, and host range experiments. Plasmid profiles and nifH hybridization patterns also revealed the occurrence of lateral plasmid transfer among distinct multilocus genotypes within species but not between species. Both species were recovered from nodules of the same plants, indicating that mechanisms other than host, spatial, or temporal isolation may account for the genetic barrier between the species. The biogeographic implications of finding an R. gallicum bv. gallicum population nodulating common bean in America are discussed.Rhizobia are soil bacteria that are capable of inducing the formation of nitrogen-fixing nodules on the roots or stems of particular legume host plants (51). Rhizobial species seem to have coevolved with their hosts at their centers of diversification (30). Common bean (Phaseolus vulgaris) originated in America; this plant was exported to the rest of the world starting in the early 16th century, and it is currently an important crop worldwide (17,18). At least five species have been reported to nodulate common bean. Rhizobium etli bv. phaseoli is the predominant P. vulgaris-nodulating species in Mexico, Colombia, and Argentina (1, 11, 42). R. etli bv. phaseoli is found in regions where common bean has been introduced, such as Spain, France, Austria, Senegal, Gambia, and Tunisia (10,21,24,33,34,44). However, in these countries other Rhizobium species also nodulate this legume. Rhizobium leguminosarum bv. phaseoli is commonly found in Europe, and it has also been reported to be present in Tunisia and Colombia (11,21,33,34). Rhizobium tropici is present in acid soils of South America (31) and has been found in France (2) and several African countries (4, 10). Rhizobium giardinii has been found only in European and Tunisian soils (3,21,34). Rhizobium gallicum has been found nodulating beans in Europe (3,21) and Tunisia (33,34), and ...

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Rhizobium etli and Rhizobium gallicum Nodulate Common Bean ( Phaseolus vulgaris ) in a Traditionally Managed Milpa Plot in Mexico: Population Genetics and Biogeographic Implications

Silva

Vinuesa

Eguiarte

et al. 2003

Appl Environ Microbiol

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“…They were inoculated with either Rhizobium tropici CIAT899, as reference strain, or with Sinorhizobium fredii 1a6 or R. etli 12a3, both isolated from the Cap Bon region in Tunisia, which were previously characterised at the phenotypic and molecular levels by Mhamdi et al [11].…”

Section: Biological Materialsmentioning

confidence: 99%

Effects of hydroponic culture system and NaCl on interactions between common bean lines and native rhizobia from Tunisian soils

Jebara¹,

Drevon²,

Aouani³

2001

Agronomie

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. Effects of hydroponic culture system and NaCl on interactions between common bean lines and native rhizobia from Tunisian soils. Agronomie, EDP Sciences, 2001, 21 (6- Abstract -Common bean (Phaseolus vulgaris L.) lines, namely BAT477, CocoT, Flamingo, DOR364 and NAG310, were inoculated with reference strain Rhizobium tropici CIAT899 or native rhizobia, namely Sinorhizobium fredii 1a6 and R. etli 12a3 and then grown in two hydroponic semi-sterile systems: (i) gravel in pots, (ii) aerated nutrient solution in bottles. In the aerated solution system, shoot plant growth was 5 to 9 times higher, depending on the symbiosis, than on gravel. A strain × line interaction was observed in the gravel system. However, such an interaction was not significant in the aerated system because of large intra-treatment variability. In the aerated system and under NaCl treatment, BAT477 inoculated with local strains (1a6 and 12a3) appeared to be a highly tolerant line. In contrast, NAG310 was the least tolerant line when inoculated by CIAT899. However, the tolerance of NAG310 to NaCl increased when it was inoculated with 12a3, although this strain conferred low nodule growth to Flamingo under NaCl. It is concluded that strain × line interactions are important and should be considered for selecting the legume most adapted to such an environmental constraint as salinity, and that the aerated solution system is efficient for selecting highly efficient rhizobial symbioses. Une interaction souche × lignée a été observée sur gravier mais non sur milieu aéré où elle semble être masquée par une importante variabilité. Sous traitement salin en milieu aéré, la lignée BAT477 inoculée avec les souches locales (1a6 et 12a3) se montre particulièrement tolérante. La lignée NAG310 est la moins tolérante quand elle est inoculée avec la souche CIAT899. Par contre, la souche 12a3 lui confère une tolérance au sel pour la production de la biomasse nodulaire contrairement à sa symbiose avec la lignée Flamingo. Il est conclu qu'il est important de prendre en considération les interactions souche × lignées pour la sélection d'une légu-mineuse adaptée à une contrainte comme la salinité, et que la culture sur solution aérée est une bonne méthode pour la sélection de symbioses rhizobiennes à fort potentiel de fixation d'azote.haricot / interactions symbiotiques / NaCl / Rhizobium / système de culture

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“…The bean seeds of BAT477, DOR364, Flamingo and NAG310 (originally supplied by V. Voysest, CIAT, Cali, Colombia) and CocoT (provided by M. Trabelsi, ESA Mateur, Tunisia), were surface sterilised, inoculated with the reference strain Rhizobium tropici CIAT899 or Sinorhizobium fredii 1a6 [12] and germinated on perlite. Seven days after sowing (DAS), the plant seedlings were reinoculated after their transfer into 1L serum bottles.…”

Section: Hydroaeroponic Culture Under Glasshousementioning

confidence: 99%

Genotypic variation in nodule conductance to the oxygen diffusion in common bean (Phaseolus vulgaris)

Jebara¹,

Drevon²

2001

Agronomie

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-Nodule conductance to O 2 diffusion in Phaseolus vulgaris was calculated from the in situ measurement of the response of nodulated-root O 2 uptake to variations of rhizospheric partial pressure of O 2 at the flowering stage for nodulated-plants grown hydroaeroponically in a glasshouse. Comparing common bean lines BAT477, CocoT, DOR364, Flamingo and NAG310, the nodule O 2 uptake at ambient pO 2 was the highest (372 µmol O 2 ⋅h -1 ⋅pl -1 ) for BAT477 in symbiosis with the reference strain Rhizobium tropici CIAT899. The maximal value (2800 µmol O 2 ⋅h -1 ⋅pl -1 ) was observed at 50 kPa O 2 . This was associated with the lowest nodule conductance (4.5 µm⋅s -1 ), though the latter was higher with the native strain Sinorhizobium fredii 1a6. The highest nodule conductance (7.5 µm⋅s -1 ) was observed for the less efficient symbiosis CocoT-CIAT899. It is argued that nodule conductance is an adaptation mechanism to respond to plant N demand in compensation for low nodulation or the high respiratory cost for nitrogen fixation in some symbioses. nodule conductance / oxygen diffusion / Phaseolus vulgaris / Rhizobiaceae / symbiosis Résumé -Variabilité génotypique de la conductance nodulaire à la diffusion de l'oxygène chez le haricot (Phaseolus vulgaris). La conductance nodulaire à la diffusion d'oxygène chez Phaseolus vulgaris a été calculée par la mesure in situ à la floraison de la réponse de la consommation d'O 2 à des variations de pO 2 rhizosphérique sur des racines nodulées de plantes en culture hydroaéro-ponique sous serre vitrée. Comparant les lignées de haricot BAT477, CocoT, DOR364, Flamingo et NAG310, la consommation nodulaire d'O 2 a été la plus élevée à la pO 2 ambiante (372 µmol O 2 ⋅h -1 ⋅pl -1 ) pour la symbiose de BAT477 avec la souche de référence Rhizobium tropici CIAT899. Elle a atteint la valeur maximale de 2800 µmol O 2 ⋅h -1 ⋅pl -1 à 50 kPa O 2 . Elle est associée à la plus faible conductance nodulaire (4.5 µm O 2 ⋅s -1 ) alors que cette dernière est plus élevée avec la souche native Sinorhizobium fredii 1a6. La conductance la plus élevée (7.5 µm O 2 ⋅s -1 ) est observée chez la symbiose CocoT-CIAT899. Il est suggéré que l'augmentation de conductance nodulaire observée chez certaines symbioses est un mécanisme d'adaptation pour répondre à la demande en azote de la plante en compensation d'une faible masse nodulaire ou d'un fort coût respiratoire lié à l'activité nitrogénase.

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Genotypic diversity and symbiotic effectiveness of rhizobia isolated from root nodules of Phaseolus vulgaris L. grown in Tunisian soils

Cited by 66 publications

References 29 publications

Rhizobium etli and Rhizobium gallicum Nodulate Common Bean ( Phaseolus vulgaris ) in a Traditionally Managed Milpa Plot in Mexico: Population Genetics and Biogeographic Implications

Rhizobium etli and Rhizobium gallicum Nodulate Common Bean ( Phaseolus vulgaris ) in a Traditionally Managed Milpa Plot in Mexico: Population Genetics and Biogeographic Implications

Effects of hydroponic culture system and NaCl on interactions between common bean lines and native rhizobia from Tunisian soils

Genotypic variation in nodule conductance to the oxygen diffusion in common bean (Phaseolus vulgaris)

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