Genotypic diversity and migration of clonal lineages of <i>Botrytis cinerea</i> from chickpea fields of Bangladesh inferred by microsatellite markers

Isenegger, Daniel; MacLeod, William J.; Ford, Rebecca; Taylor, P. W. J.

doi:10.1111/j.1365-3059.2008.01885.x

Cited by 28 publications

(19 citation statements)

References 44 publications

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“…Results from 20 runs for each K were visualized in CLUMPAK (Kopelman et al 2015). Each isolate was assigned to a given cluster when the proportion of its genome in that cluster (q K ) was higher than an arbitrary cut-off value of 80% (Liu et al 2003;Fukunaga et al 2005;Isenegger et al 2008;Vigouroux et al 2008). Isolates that could not meet the arbitrary cut-off were assigned to the 'admixed' group.…”

Section: Population Structurementioning

confidence: 99%

Three genetic groups of the Eucalyptus stem canker pathogen Teratosphaeria zuluensis introduced into Africa from an unknown source

Jimu

Chen

Wingfield

et al. 2015

Antonie van Leeuwenhoek

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The Eucalyptus stem canker pathogen Teratosphaeria zuluensis was discovered in South Africa in 1988 and it has subsequently been found in several other African countries as well as globally. In this study, the population structure, genetic diversity and evolutionary history of T. zuluensis were analysed using microsatellite markers to gain an enhanced understanding of its movement in Africa. Isolates were collected from several sites in Malawi, Mozambique, Uganda and Zambia. Data obtained were compared with those previously published for a South African population. The data obtained from 334 isolates, amplified across eight microsatellite loci, were used for assignment, differentiation and genetic diversity tests. STRUCTURE analyses, θ st and genetic distances revealed the existence of two clusters, one dominated by isolates from South Africa and the other by isolates from the Zambezi basin including Malawi, Mozambique and Zambia. High levels of admixture were found within and among populations, dominated by the Mulanje population in Malawi. Moderate to low genetic diversity of the populations supports the previously held view that the pathogen was introduced into Africa. The clonal nature of the Ugandan population suggests a very recent introduction, most likely from southern Africa.

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Section: Population Structurementioning

confidence: 99%

Three genetic groups of the Eucalyptus stem canker pathogen Teratosphaeria zuluensis introduced into Africa from an unknown source

Jimu

Chen

Wingfield

et al. 2015

Antonie van Leeuwenhoek

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“…; Isenegger et al. ; Váczy et al. ), clone mates were identified by calculating the probability ( P GEN ) of each multilocus genotype (MLG) arising through sexual instead of clonal reproduction using the software genclone V2.0 (Arnaud‐Haond and Belkhir ).…”

Section: Methodsmentioning

confidence: 99%

Presence of Transposons and Mycoviruses in Botrytis cinerea Isolates Collected from a German Grapevine Growing Region

Kecskeméti

Brathuhn

Kogel

et al. 2014

Journal of Phytopathology

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Transposons and infection of fungal strains with mycoviruses can have significant effects on distinctive phenotypic traits of phytopathogenic fungi such as mycelial growth and sporulation, pathogenicity or fungicide resistance. Two transposable elements (TE), Boty and Flipper, are known to be associated with the ubiquitous fungus Botrytis cinerea. In addition, the presence of two types of ssRNAsRNA viruses, BVX and BVF, has been reported in B. cinerea. In this study, we assessed the genetic diversity of B. cinerea isolates, all sampled within a small‐sized German viticultural area (‘Rheingau’) by examining and classifying them according to the presence of TEs and mycoviruses. A subset of the isolates was further analysed with microsatellite markers to determine the origin of particular isolates with or without one or both mycoviruses. Virtually all isolates (98%) sampled in two different years (2008 and 2010) were screened positive for the presence of a transposon. Presence of one or both B. cinerea mycoviruses was confirmed for 37% of the analysed isolates sampled in 2010, representing the first record of B. cinerea mycoviruses in German isolates. Assignment on individual B. cinerea isolates to different genetic groups was independent of the presence or absence of a mycovirus or a transposable element, respectively. Furthermore, we found no correlation between the presence of either a mycovirus or a transposable element and different viticultural management practices, soil properties or levels of nitrogen fertilization applied to the respective vineyards. However, mycelial growth of B. cinerea strains containing mycovirus BVF was significantly reduced at lower temperatures.

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“…Some of the resistant chickpea lines such as ICC1069, P349-2 and NEC2451 have been widely used in breeding (Haware et al 1992;Chaturvedi et al 1995) but higher levels of host resistance still need to be identified . Furthermore, these resistances are unlikely to hold in the longer term as pathogen diversity indicates likely breakdown of host resistance (Isenegger et al 2008). …”

Section: Breeding For Fungal Disease Resistancementioning

confidence: 99%

“…B. cinerea isolates collected from India and Nepal have been differentiated based on their morphocultural characters and effects on different chickpea genotypes (Singh and Bhan 1986;Rewal and Grewal 1989a, b;. Nine simple sequence repeat markers were used on B. cinerea (Fournier et al 2002) to study population structure of a total of 51 alleles were amplified among 146 B. cinerea isolates of chickpea from Bangladesh, India and Nepal, which revealed a high amount of within-population and overall genetic diversity (Isenegger et al 2005(Isenegger et al , 2008. Different methods have been applied to screen germplasm for BGM resistance under in vitro, greenhouse and field conditions (Pande et al 2002(Pande et al , 2006aGurha et al 2003).…”

Section: Breeding For Fungal Disease Resistancementioning

confidence: 99%

Breeding for biotic stress resistance in chickpea: progress and prospects

Li¹,

Rodda²,

Gnanasambandam

et al. 2015

Euphytica

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Chickpea (Cicer arietinum L.) is the third most economically important food legume in the world. Its yield potential is often limited by various biotic stresses, including fungal and viral diseases, insects, nematodes and parasitic weeds. Incorporating genetic resistance into cultivars is the most effective and economical way of controlling biotic stresses and this is a major objective in many breeding programs. Extensive searches for resistances have been conducted by screening commercial varieties, landraces and closely related species. Resistances to disease such as Ascochyta blight and Fusarium wilt have been identified and molecular tools are being used to increase the efficiency of gene transfer from wild species into chickpea elite genotypes. Quantitative trait loci for resistance genes have been located on linkage maps and molecular markers associated with these loci can potentially be used for efficient pyramiding of the traits. Significant chickpea genomic resources have been developed in order to investigate resistance genes. Such resources include an integrated genetic map, expressed sequence tag libraries, bacterial artificial chromosome libraries, microarrays and draft genome sequences. Although these resources have yet to be used to improve chickpea cultivars in the field, this is likely to change in the near future. These genomic resources, as well as high-resolution phenotyping tools and cutting-edge technologies such as next-generation sequencing, promise to increase efficiency as work to identify valuable candidate genes continues.

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Genotypic diversity and migration of clonal lineages of Botrytis cinerea from chickpea fields of Bangladesh inferred by microsatellite markers

Cited by 28 publications

References 44 publications

Three genetic groups of the Eucalyptus stem canker pathogen Teratosphaeria zuluensis introduced into Africa from an unknown source

Three genetic groups of the Eucalyptus stem canker pathogen Teratosphaeria zuluensis introduced into Africa from an unknown source

Presence of Transposons and Mycoviruses in Botrytis cinerea Isolates Collected from a German Grapevine Growing Region

Breeding for biotic stress resistance in chickpea: progress and prospects

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