Genotypic Differences in Antioxidative Stress and Salt Tolerance of Three Poplars Under Salt Stress

Wang, Ruigang; Chen, Shao Liang; Ma, Haiyuan; Liu, Liyuan; Li, He; Weng, Haijiao; Hao, Zhiyong; Yang, Shuang

doi:10.1007/s11461-005-0019-8

Cited by 19 publications

(17 citation statements)

References 31 publications

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“…5; Table 3). We have shown that iso-enzymatic and total activity of leaf SOD, APX and POD in P. euphratica were rapidly increased after the onset of salt stress (Wang et al 2006(Wang et al , 2007(Wang et al , 2008. In this study, increases in transcripts of antioxidant enzymes following ST treatment enabled P. euphratica plants to initiate an effective antioxidant defense to deal with prolonged saline conditions.…”

Section: Discussionmentioning

confidence: 62%

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Salt-induced expression of genes related to Na+/K+ and ROS homeostasis in leaves of salt-resistant and salt-sensitive poplar species

et al. 2010

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Using the Affymetrix poplar genome array, we explored the leaf transcriptome of salt-tolerant Populus euphratica Oliv. and salt-sensitive P. popularis 35-44 (P. popularis) under control and saline conditions. Our objective was to clarify the genomic differences in regulating K(+)/Na(+) and reactive oxygen species (ROS) homeostasis between the two species. Compared to P. popularis, salt-tolerant P. euphratica responses to salinity involved induction of a relatively larger number of probesets after short-term (ST) exposure to 150 mM NaCl (24 h) and relatively fewer probesets after a long-term (LT) exposure to salinity (200 mM NaCl, 28 days). Compared to P. popularis, leaves of the control P. euphratica plants exhibited a higher transcript abundance of genes related to Na(+)/H(+) antiport (Na(+)/H(+) antiporters, H(+) pumps) and K(+) uptake and transport. Notably, the expression of these genes did not decrease (with a few exceptions) during salt treatment. Regarding ROS homeostasis, P. euphratica exhibited rapid up-regulation of a variety of antioxidant enzymes after exposure to ST salinity, indicating a rapid adaptive response to salt stress. However, the effect of NaCl on transcription in P. popularis leaves was more pronounced after exposure to prolonged salinity. LT-stressed P. popularis up-regulated some genes mediating K(+)/Na(+) homeostasis but decreased transcription of main scavengers of superoxide radicals and H(2)O(2) except for some isoforms of a few scavengers. Mineral and ROS analyses show that NaCl induced a marked increase of leaf Na(+) and H(2)O(2) in LT-stressed plants of the two species and the effects were even more pronounced in the salt-sensitive poplar. We place the transcription results in the context of our physiological measurements to infer some implications of NaCl-induced alterations in gene expression related to K(+)/Na(+) and ROS homeostasis.

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Section: Discussionmentioning

confidence: 62%

“…LT stress caused a drastic increase of H 2 O 2 in P. popularis (Fig. 2), which resulted in oxidative damage to leaf cells (Wang et al 2006(Wang et al , 2007(Wang et al , 2008. However, H 2 O 2 levels were maintained at a lower level in LTstressed P. euphratica (Fig.…”

Section: Discussionmentioning

confidence: 97%

Salt-induced expression of genes related to Na+/K+ and ROS homeostasis in leaves of salt-resistant and salt-sensitive poplar species

et al. 2010

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“…Under the same saline conditions, P. popularis and P. cv. I-214 exhibited a clear symptom of leaf injury whereas there was no leaf necrosis in P. euphratica (Chen et al 2002b;Wang et al 2006). We found that the increased membrane permeability in salt-sensitive P. popularis leaves was associated with salt-induced lipid peroxidation, which was likely due to the drastic decline of SOD and POD activity .…”

Section: Introductionmentioning

confidence: 72%

Leaf photosynthesis, fluorescence response to salinity and the relevance to chloroplast salt compartmentation and anti-oxidative stress in two poplars

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Chen

Deng

et al. 2007

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In a 16-day study, the effect of increasing soil NaCl on leaf photosynthesis, chlorophyll a fluorescence, chloroplast ion compartmentation, variations of SOD (superoxide dismutase) and POD (peroxidase) isoenzymes and the relevance to salt resistance were investigated in seedlings of Populus euphratica Oliv. (P. euphratica) (saltresistant) and rooted cuttings of P. ''popularis 35-44'' (P. popularis) (salt-sensitive). Initial salinity caused a rapid decline of net photosynthetic rate (Pn) and unit transpiration rate (TRN) in P. euphratica, resulting from the NaClinduced stomatal closure. In a longer-term of salinity, CO 2 assimilation in P. popularis was severely reduced whereas stressed P. euphratica maintained a relatively higher and constant level of Pn. Pn-Ci curves showed that salt stress (12 days) reduced CO 2 saturation point (CSP), CO 2 saturated Pn (CSP n ), and carboxylation efficiency (CE), but increased CO 2 compensation point (CCP) in the two genotypes. Similarly, salinity lowered light saturation point (LSP), light saturated Pn (LSP n ), and apparent quantum yield (AQY) in both genotypes but the inhibitory effect of NaCl on light reaction was more pronounced in P. popularis, as compared to P. euphratica. Chlorophyll a fluorescence data indicated that a longer-term of salt stress (12 days) exhibited a marked influence on fluorescence parameters of P. popularis in both dark-and light-adapted states: (a) NaCl inhibited the maximal efficiency of PSII photochemistry (Fv/Fm) due to the salt-induced increase of Fo (the minimal fluorescence) and the marked decline of Fm (the maximal fluorescence); (b) salinity decreased coefficient of photochemical quenching (qP) but markedly elevated coefficient of nonphotochemical quenching (qN) in the light-adapted state. In contrast, there were no corresponding changes of chlorophyll a fluorescence in salinised P. euphratica. X-ray microanalysis results showed that salinity caused salt accumulation in the chloroplasts of P. popularis in which Na + and Cl -increased up to 42 and 221 mmol dm -3 , respectively. Great buildup of Na + and Cl -in chloroplasts of P. popularis may exhibit direct and indirect restrictions on dark and light reactions. The activity of SOD isoenzymes (CuZn-SOD I and CuZn-SOD II) and POD isoenzymes in P. popularis decreased with increasing exposure period, and leaf malondialdehyde (MDA) content and membrane permeability (MP) increased correspondingly. In contrast to P. popularis, stressed P. euphratica maintained activity of SOD and POD isoenzymes and there was no significant increase of MDA and MP during the period of salt stress. In conclusion, P. euphratica plants exhibited a higher capacity to maintain the activity of anti-oxidant enzymes and restrict salt accumulation in the chloroplasts, the photosynthesis processes were less restricted consequently.

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“…So, mere consideration of single saltstress effect is not enough and the effects of multi-salts are needed to identify the salt resistance of the plant. The main saline components in saline soil are Na 2 SO 4 and NaCl (Wang et al, 2005). Their effects should be comprehensively considered.…”

Section: Effects Of Different Salts On Plantmentioning

confidence: 99%

Investigation of anti-salt stress on tetraploid Robinia pseudoacacia

et al. 2009

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Tetraploid Robinia pseudoacacia was used as a main test material and diploid R. pseudoacacia was used as the control. The indices of shape, physiology and biochemistry, photosynthesis and anatomic structure of the young plants were investigated under salt stress (NaCl and Na 2 SO 4 ). The treatment time was 30 d with an interval time of 7 d. Before and after treatment, the indices were measured. Results show that: 1) the growth of diploid R. pseudoacacia inhibited an evident symptom of salt damage and the leaf moisture content was lower under salt stress than that of control. But the tetraploid R. pseudoacacia was contrary. 2) The relative electric conductivity and proline (Pro) of tetraploid R. pseudoacacia increased slightly and had no significant difference compared with its control, which was contrary to diploid R. pseudoacacia. At the same time, three protective enzymes including perocidase (POD), superoxide (SOD) and catalase (CAT) kept higher activities at a post stage of salt stress to tetraploid R. pseudoacacia, which enhanced its anti-salt characteristics. Diploid R. pseudoacacia was sensitive to salt and had contrary information. 3) Salt stress had little influence to photosynthesis of tetraploid R. pseudoacacia. The net photosynthetic rate (P n ) and intercellular CO 2 concentration (C i ) had no significant changes, but those of diploid R. pseudoacacia decreased singificantly. 4) After salt stress, the anatomic structure of tetraploid R. pseudoacacia had a positive reaction, including the palisade parenchyma of diachyma, was prolonged and arranged more tightly. The spongy parenchyma was shrunk and was arranged tightly, which was contrary with diploid R. pseudoacacia. These data demonstrate that tetraploid R. pseudoacacia had superior anti-salt performance.

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Genotypic Differences in Antioxidative Stress and Salt Tolerance of Three Poplars Under Salt Stress

Cited by 19 publications

References 31 publications

Salt-induced expression of genes related to Na+/K+ and ROS homeostasis in leaves of salt-resistant and salt-sensitive poplar species

Salt-induced expression of genes related to Na+/K+ and ROS homeostasis in leaves of salt-resistant and salt-sensitive poplar species

Leaf photosynthesis, fluorescence response to salinity and the relevance to chloroplast salt compartmentation and anti-oxidative stress in two poplars

Investigation of anti-salt stress on tetraploid Robinia pseudoacacia

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