Genotypic characterization of psittacid herpesvirus isolates from Brazil

Luppi, Marcela Miranda; Luiz, Ana Paula; Coelho, Fabiana Magalhães; Ecco, Roselene; Fonseca, Flávio Guimarães da; Resende, Maria Aparecida de

doi:10.1016/j.bjm.2015.11.017

Cited by 3 publications

(3 citation statements)

References 14 publications

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“…Even so, there are only sporadic reports on PD occurrence in parrots in Brazil [14] and there is no information on the genotypes circulating in the country. So far, in captive birds, only Genotype 1 was found in 18 Amazon individuals [37]. Negative results for avipoxvirus were also reported in 29 captive A. vinacea using the same primers in a conventional PCR [38].…”

Section: Discussionmentioning

confidence: 91%

Molecular Survey of Pathogens in Wild Amazon Parrot Nestlings: Implications for Conservation

Vaz

Sipinski²,

Seixas

et al. 2021

Diversity

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South America presents the greatest Psittacidae diversity in the world, but also has the highest numbers of threatened parrot species. Recently, exotic viruses have been detected in captive native psittacine birds in Brazil, however, their impacts on the health of wild parrots are still unknown. We evaluated the presence of Chlamydia psittaci, Psittacid alphaherpesvirus 1 (PsHV-1), avipoxvirus and beak and feather disease virus (BFDV) in wild Amazona aestiva, A.brasiliensis and A.pretrei nestlings and in wild caught A.aestiva nestlings seized from illegal trade. Samples were collected from 205 wild nestlings and 90 nestlings from illegal trade and pathogen-specific PCR was performed for each sample. Chlamydia DNA prevalence was 4.7% in A.aestiva and 2.5% in A.brasiliensis sampled from the wild. Sequencing revealed that the C.psittaci sample belonged to the genotype A. PsHV-1, avipoxvirus and BFDV DNA was not detected. These results have conservation implications since they suggest that wild parrot populations have a low prevalence of the selected pathogens and, apparently, they were not reached by the exotic BFDV. Stricter health protocols should be established as condition to reintroduction of birds to the wild to guarantee the protection of Neotropical parrots.

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Section: Discussionmentioning

confidence: 91%

Molecular Survey of Pathogens in Wild Amazon Parrot Nestlings: Implications for Conservation

Vaz

Sipinski²,

Seixas

et al. 2021

Diversity

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“…To further confirm the deletion in the recombinant mutants, genomic DNA was extracted from the mutant virus and the wild-type strain, and it was analyzed for RFLPs using the restriction enzyme Hin dIII (Takara, Dalian, China) as described previously [ 34 ]. After enzymatic digestion, the resulting DNA fragments were separated on a 0.7 % agarose gel at room temperature for approximately 15 h under a constant voltage of 20 V.…”

Section: Methodsmentioning

confidence: 99%

Bovine herpesvirus 1 tegument protein UL21 plays critical roles in viral secondary envelopment and cell-to-cell spreading

et al. 2017

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Bovine herpesvirus 1 (BoHV-1) UL21 is a tegument protein thought to be indispensable for efficient viral growth but its precise function in BoHV-1 is currently unknown. To determine the function of UL21 in BoHV-1 replication, we constructed a mutant virus bearing a UL21 deletion (vBoHV-1-∆UL21) and its revertant virus, vBoHV-1-∆UL21R, in which the UL21 gene was restored using a bacterial artificial chromosome system. The replication of vBoHV-1-∆UL21 was 1,000-fold lower and its plaque size was 85% smaller than those of the wild-type virus (BoHV-1). An ultrastructural analysis showed that deletion of UL21 led to an un-enveloped capsid accumulation in the cytoplasm, whereas nucleocapsid egress was not impaired, suggesting that UL21 is critical for secondary envelopment in BoHV-1. Co-immunoprecipitation assays revealed that HA-tagged UL21 pulled down UL16, suggesting that these two proteins form a complex, and this was further confirmed by a co-immunofluorescence assay. Taken together, these data provide evidence that UL21 plays critical roles in BoHV-1 secondary envelopment, and UL16 is likely to be involved in these activities.

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“…At first, this suggests a common exposure to the same agent, corroborated by the provision of the facilities and management of avian species practiced in the farm, which favors viral dissemination. Further analysis of other genetic regions, including UL16 ORF, could provide more consistent data on circulating viral genotypes (Luppi et al, 2016;Tomaszewski et al, 2003).…”

Section: Sequence and Phylogenetic Analysesmentioning

confidence: 99%

Pacheco’s Disease Outbreak with High Mortality in Brazilian Captive Psittacine Birds

Barbosa¹,

Gregori²,

Monteiro³

et al. 2022

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Psittacid Alphaherpesvirus 1 (PSHv-1) is the causative agent of Pacheco's disease that infects psittacine birds, highly transmissible and presenting high mortality rate. Here we describe the investigation of 98 sudden deaths of birds, belonging to 8 Brazilian native psittacine species, divided in two outbreaks, one year apart between 2016 and 2017, in a breeding farm in São Paulo State, Brazil. PSHv-1 was detected in 6 species (61 birds) through Nested-PCR, that tested negative for Influenza, Paramyxovirus, Flavivirus, and Adenovirus. Genetic sequencing and phylogenetic analysis support these results.

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Genotypic characterization of psittacid herpesvirus isolates from Brazil

Cited by 3 publications

References 14 publications

Molecular Survey of Pathogens in Wild Amazon Parrot Nestlings: Implications for Conservation

Molecular Survey of Pathogens in Wild Amazon Parrot Nestlings: Implications for Conservation

Bovine herpesvirus 1 tegument protein UL21 plays critical roles in viral secondary envelopment and cell-to-cell spreading

Pacheco’s Disease Outbreak with High Mortality in Brazilian Captive Psittacine Birds

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