Genotypic characterization of extended-spectrum β-lactamase producing urinary isolates among pregnant women in Ho municipality, Ghana

Adedze-Kpodo, Robert K; Feglo, Patrick Kwame; Agboli, Eric; Asmah, Richard Harry; Kwadzokpui, Precious Kwablah

doi:10.1016/j.heliyon.2022.e12513

Cited by 3 publications

(3 citation statements)

References 28 publications

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“…The percentage of Staphylococcus aureus isolates in the current study is almost similar to the results of the above mentioned two studies [22,23] . While these results are different with other studies that had lower percentage than this study [24,25].…”

Section: Figure (4): Bacterial Isolates Of Uti In Pregnant Womensupporting

confidence: 90%

Bacterial Infections in Urinary Tract among Pregnant Women in Nassiriyah City, Iraq

Mousa,

Abd Al-Amir

2023

UTJsci

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Abstract—Urinary tract infection (UTI) is a common medical complication in pregnant women .It is associated with severe consequences for both the fetus and the mother. This study designed to detect the frequency of microorganisms causing UTI in pregnancy women. One hundred and twenty participants of pregnancy women included in this study. Urine samples were collected from each participant and cultured on blood and MacConkey agars ,followed by macro and microscopic identification and biochemical tests. API 20E and API Staph compact systems were used as a confirmatory tests . The results showed that the ratio of positive urine culture was 53 (44.2% ) , and the highest positive urine cultures were detected in the second trimester 23/53 (43.3%). Also, gram positive bacteria particularly Staphyiococcusspp were the most common frequency (60.2 %) in this study , and Staphylococcus aureus was the most common microorganism isolated from pregnancy women with UTIs followed by Escherichia coli (19%) . UTI is a major health problem among pregnant women especially during the last two trimesters of pregnancy. Staphylococcus aureus is the dominant pathogen nowadays causing UTIs in different trimesters.

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Section: Figure (4): Bacterial Isolates Of Uti In Pregnant Womensupporting

confidence: 90%

Bacterial Infections in Urinary Tract among Pregnant Women in Nassiriyah City, Iraq

Mousa,

Abd Al-Amir

2023

UTJsci

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“…Focusing on the beta-lactamase genes detected by real-time PCR, the quantitative dominance of bla CTX-M is not further surprising, as this genetic resistance determinant has been repeatedly described to be highly prevalent in Ghana [ 79 , 80 , 81 , 82 , 83 , 84 , 85 , 86 , 87 , 88 , 89 , 90 , 91 , 92 , 93 ] and also Ivory Coast [ 41 ]. In contrast, carbapenemases are still less frequently reported from Ghana and Ivory Coast with resistance genes like, e.g., bla IMP , bla NDM , bla OXA-23 -like, bla OXA-48 -like, including bla OXA-181 , bla OXA-58 -like and bla VIM being associated with varying low prevalence rates or outbreaks [ 42 , 94 , 95 , 96 , 97 , 98 , 99 , 100 , 101 , 102 , 103 , 104 , 105 ].…”

Section: Discussionmentioning

confidence: 99%

Screening for Resistant Bacteria, Antimicrobial Resistance Genes, Sexually Transmitted Infections and Schistosoma spp. in Tissue Samples from Predominantly Vaginally Delivered Placentae in Ivory Coast and Ghana

Franz,

Hahn,

Hagen

et al. 2023

Pathogens

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Medical complications during pregnancy have been frequently reported from Western Africa with a particular importance of infectious complications. Placental tissue can either become the target of infectious agents itself, such as, e.g., in the case of urogenital schistosomiasis, or be subjected to contamination with colonizing or infection-associated microorganisms of the cervix or the vagina during vaginal delivery. In the retrospective cross-sectional assessment presented here, the quantitative dimension of infection or colonization with selected resistant or pathogenic bacteria and parasites was regionally assessed. To do so, 274 collected placental tissues from Ivory Coastal and Ghanaian women were subjected to selective growth of resistant bacteria, as well as to molecular screening for beta-lactamase genes, Schistosoma spp. and selected bacterial causative agents of sexually transmitted infections (STI). Panton–Valentine-negative methicillin-resistant Staphylococcus aureus (MRSA) was grown from 1.8% of the tissue samples, comprising the spa types t008 and t688, as well as the newly detected ones, t12101 (n = 2) and t12102. While the culture-based recovery of resistant Enterobacterales and nonfermentative rod-shaped Gram-negative bacteria failed, molecular assessments confirmed beta-lactamase genes in 31.0% of the samples with multiple detections of up to four resistance genes per sample and blaCTX-M, blaIMP, blaGES, blaVIM, blaOXA-58-like, blaNDM, blaOXA-23-like, blaOXA-48-like and blaKPC occurring in descending order of frequency. The beta-lactamase genes blaOXA-40/24-like, blaNMC_A/IMI, blaBIC, blaSME, blaGIM and blaDIM were not detected. DNA of the urogenital schistosomiasis-associated Schistosoma haematobium complex was recorded in 18.6% of the samples, but only a single positive signal for S. mansoni with a high cycle-threshold value in real-time PCR was found. Of note, higher rates of schistosomiasis were observed in Ghana (54.9% vs. 10.3% in Ivory Coast) and Cesarean section was much more frequent in schistosomiasis patients (61.9% vs. 14.8% in women without Schistosoma spp. DNA in the placenta). Nucleic acid sequences of nonlymphogranuloma-venereum-associated Chlamydia trachomatis and of Neisseria gonorrhoeae were recorded in 1.1% and 1.9% of the samples, respectively, while molecular attempts to diagnose Treponema pallidum and Mycoplasma genitalium did not lead to positive results. Molecular detection of Schistosoma spp. or STI-associated pathogens was only exceptionally associated with multiple resistance gene detections in the same sample, suggesting epidemiological distinctness. In conclusion, the assessment confirmed considerable prevalence of urogenital schistosomiasis and resistant bacterial colonization, as well as a regionally expected abundance of STI-associated pathogens. Continuous screening offers seem advisable to minimize the risks for the pregnant women and their newborns.

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“…However, our inability to assess animal contact during the study precludes us from definitively establishing this connection. Notably, ESBL-producing bacteria harboring blaCTX-M-1 genes have been reported from diverse sources in Ghana, including food and farm animals and their settings [48,49], patients [50,51], healthcare facility settings [52], as well as potentially healthy community individuals [53] and their environment [54,55].…”

Section: Plos Onementioning

confidence: 99%

Extended-spectrum beta-lactamases in clinical isolates of Escherichia coli and Klebsiella pneumoniae recovered from patients at the Tamale Teaching Hospital, Ghana

Tetteh,

Ablordey,

Obeng-Nkrumah

et al. 2024

PLoS ONE

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Introduction Extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli and Klebsiella pneumoniae are pathogens of significant public health interest for which new antibiotics are urgently needed. Aim To determine the prevalence of ESBLs in E. coli and K. pneumoniae isolates from patients attending the Tamale Teaching Hospital (TTH) in Ghana. Methodology The study was a cross-sectional study involving convenience sampling of E. coli and K. pneumoniae isolates from consenting patients’ clinical specimens, between April and June 2015. Antimicrobial susceptibility test was performed, and ESBL-producer phenotypes were further screened for BlaTEM, BlaSHV, and BlaCTX-M genes. Patients’ clinical data were additionally collected using a structured questionnaire. Results Of the 150 non-duplicate E. coli and K. pneumoniae isolates identified, 140 were confirmed as E. coli (84%, n = 117) and K. pneumoniae (16%, n = 23). Of these, sixty-two (44%) [E. coli (84%; n = 52); K. pneumoniae (16%; n = 10)] phenotypically expressed ESBLs. The proportion of ESBL-producing isolates was higher in adults (15–65 years) than in neonates (< 28 days) (p = 0.14). Most of the isolates showed a high percentage resistance to ampicillin (96%) and tetracycline (89%), but a relatively lower resistance to amikacin (36%). No isolate was resistant to meropenem. More ESBL producers were multidrug resistant compared to non-ESBL-producers [23% (14/62) versus 18% (14/78); p = 0.573]. Overall, 74% (n = 46) of the ESBL genotypes expressed BlaCTX-M-1 genes, followed by 63% (n = 39) BlaTEM, and 16% (n = 10) BlaSHV. The study showed a high prevalence of ESBL-positive E. coli and K. pneumoniae, mostly CTX-M-1 producers at TTH. Conclusion Routine laboratory ESBL screening is warranted to inform patient management.

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Genotypic characterization of extended-spectrum β-lactamase producing urinary isolates among pregnant women in Ho municipality, Ghana

Cited by 3 publications

References 28 publications

Bacterial Infections in Urinary Tract among Pregnant Women in Nassiriyah City, Iraq

Bacterial Infections in Urinary Tract among Pregnant Women in Nassiriyah City, Iraq

Screening for Resistant Bacteria, Antimicrobial Resistance Genes, Sexually Transmitted Infections and Schistosoma spp. in Tissue Samples from Predominantly Vaginally Delivered Placentae in Ivory Coast and Ghana

Extended-spectrum beta-lactamases in clinical isolates of Escherichia coli and Klebsiella pneumoniae recovered from patients at the Tamale Teaching Hospital, Ghana

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